BRENDA - Enzyme Database
show all sequences of 1.5.1.17

Purification and properties of alanopine dehydrogenase from the adductur muscle of the oyster, Crassostrea gigas (mollusca, bivalvia)

Fields, J.H.A.; Hochachka, P.W.; Eur. J. Biochem. 114, 615-621 (1981)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-oxoglutarate
uncompetitive with respect to Ala and pyruvate
Crassostrea gigas
ADP
competitive with respect to NADH
Crassostrea gigas
AMP
competitive with respect to NADH
Crassostrea gigas
ATP
competitive with respect to NADH
Crassostrea gigas
NAD+
competitive with respect to NADH
Crassostrea gigas
oxamate
-
Crassostrea gigas
succinate
-
Crassostrea gigas
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
NADH
-
Crassostrea gigas
0.45
-
pyruvate
-
Crassostrea gigas
3.45
-
2-oxobutanoate
-
Crassostrea gigas
3.49
-
glyoxylate
-
Crassostrea gigas
6.48
-
Hydroxypyruvate
-
Crassostrea gigas
16
-
2-oxovalerate
-
Crassostrea gigas
96
-
Gly
-
Crassostrea gigas
98
-
L-Ala
-
Crassostrea gigas
150
-
2-aminobutanoate
-
Crassostrea gigas
680
-
Ser
-
Crassostrea gigas
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46500
-
1 * 46500, SDS-PAGE
Crassostrea gigas
47200
-
gel filtration
Crassostrea gigas
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Crassostrea gigas
-
-
-
Purification (Commentary)
Commentary
Organism
-
Crassostrea gigas
Source Tissue
Source Tissue
Commentary
Organism
Textmining
adductor
-
Crassostrea gigas
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
691
-
-
Crassostrea gigas
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Gly + pyruvate + NADH
-
13370
Crassostrea gigas
Strombine + NAD+ + H2O
-
-
-
-
L-2-Aminobutyrate + pyruvate + NADH
-
13370
Crassostrea gigas
?
-
-
-
-
L-Ala + 2-oxobutanoate + NADH
at 68% of the activity with pyruvate
13370
Crassostrea gigas
?
-
-
-
-
L-Ala + 2-oxopentanoate + NADH
at 58% of the activity with pyruvate
13370
Crassostrea gigas
?
-
-
-
-
L-Ala + glyoxylate + NADH
at 10.5% of the activity with pyruvate
13370
Crassostrea gigas
?
-
-
-
-
L-Ala + hydroxypyruvate + NADH
at 3.3% of the activity with pyruvate
13370
Crassostrea gigas
?
-
-
-
-
L-Ala + pyruvate + NADH
-
13370
Crassostrea gigas
2,2'-Iminodipropanoate + NAD+ + H2O
-
-
-
-
L-Ser + pyruvate + NADH
-
13370
Crassostrea gigas
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 46500, SDS-PAGE
Crassostrea gigas
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Crassostrea gigas
NADH
-
Crassostrea gigas
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Crassostrea gigas
NADH
-
Crassostrea gigas
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-oxoglutarate
uncompetitive with respect to Ala and pyruvate
Crassostrea gigas
ADP
competitive with respect to NADH
Crassostrea gigas
AMP
competitive with respect to NADH
Crassostrea gigas
ATP
competitive with respect to NADH
Crassostrea gigas
NAD+
competitive with respect to NADH
Crassostrea gigas
oxamate
-
Crassostrea gigas
succinate
-
Crassostrea gigas
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
NADH
-
Crassostrea gigas
0.45
-
pyruvate
-
Crassostrea gigas
3.45
-
2-oxobutanoate
-
Crassostrea gigas
3.49
-
glyoxylate
-
Crassostrea gigas
6.48
-
Hydroxypyruvate
-
Crassostrea gigas
16
-
2-oxovalerate
-
Crassostrea gigas
96
-
Gly
-
Crassostrea gigas
98
-
L-Ala
-
Crassostrea gigas
150
-
2-aminobutanoate
-
Crassostrea gigas
680
-
Ser
-
Crassostrea gigas
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46500
-
1 * 46500, SDS-PAGE
Crassostrea gigas
47200
-
gel filtration
Crassostrea gigas
Purification (Commentary) (protein specific)
Commentary
Organism
-
Crassostrea gigas
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
adductor
-
Crassostrea gigas
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
691
-
-
Crassostrea gigas
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Gly + pyruvate + NADH
-
13370
Crassostrea gigas
Strombine + NAD+ + H2O
-
-
-
-
L-2-Aminobutyrate + pyruvate + NADH
-
13370
Crassostrea gigas
?
-
-
-
-
L-Ala + 2-oxobutanoate + NADH
at 68% of the activity with pyruvate
13370
Crassostrea gigas
?
-
-
-
-
L-Ala + 2-oxopentanoate + NADH
at 58% of the activity with pyruvate
13370
Crassostrea gigas
?
-
-
-
-
L-Ala + glyoxylate + NADH
at 10.5% of the activity with pyruvate
13370
Crassostrea gigas
?
-
-
-
-
L-Ala + hydroxypyruvate + NADH
at 3.3% of the activity with pyruvate
13370
Crassostrea gigas
?
-
-
-
-
L-Ala + pyruvate + NADH
-
13370
Crassostrea gigas
2,2'-Iminodipropanoate + NAD+ + H2O
-
-
-
-
L-Ser + pyruvate + NADH
-
13370
Crassostrea gigas
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 46500, SDS-PAGE
Crassostrea gigas
Other publictions for EC 1.5.1.17
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743404
Murphy
Changes to coral health and m ...
Montipora capitata
PeerJ
4
e1956
2016
1
1
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1
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1
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1
-
1
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-
1
-
-
-
-
3
3
-
-
-
741868
Lagana
-
Anaerobiosis and metabolic pl ...
Pinna nobilis
Biochem. Syst. Ecol.
56
138-143
2014
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
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5
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1
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1
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1
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2
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2
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1
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1
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5
-
-
1
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
742962
Gohlke
Binding region of alanopine d ...
Arenicola marina
J. Chem. Inf. Model.
53
2493-2498
2013
-
-
-
-
-
-
1
3
-
-
-
1
-
2
-
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1
-
-
-
-
-
3
1
-
-
-
2
-
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3
1
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-
3
-
-
-
-
1
1
3
-
-
-
1
-
-
-
1
-
-
-
-
3
1
-
-
-
2
-
-
-
-
-
3
3
-
-
-
13290
Carvajal
-
Lactate dehydrogenase, alanopi ...
Concholepas concholepas
Comp. Biochem. Physiol. B
108
543-550
1994
-
-
-
-
-
-
-
5
-
-
1
-
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
1
-
-
-
-
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-
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-
-
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5
-
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1
-
-
-
-
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-
1
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
13292
Sato
-
Distribution of opine dehydrog ...
Anthopleura japonica, Anthopleura nigrescens, Azumapecten farreri nipponensis, Buccinum isaotakii, Cellana grata, Chlorostoma lischkei, Crassostrea gigas, Fusitriton oregonensis, Haliotis discus hannai, Heterololigo bleekeri, Liolophura japonica, Littorina brevicula, Meretrix lusoria, Mesocentrotus nudus, Mizuhopecten yessoensis, Mytilus edulis, Neptunea arthritica, no activity in Aplysia juliana, no activity in Aplysia kurodai, no activity in Asterias amurensis, no activity in Asterina pectinifera, no activity in Aurelia aurita, no activity in Balanus cariosus, no activity in Halichondria japonica, no activity in Halocynthia roretzi, no activity in Hemigrapsus sanguineus, no activity in Hexagrammos otakii, no activity in Octopus membranaceus, no activity in Oncorhynchus keta, no activity in Pagurus samuelis, no activity in Pollicipes mitella, no activity in Pugettia quadridens, no activity in Solaster paxillatus, no activity in Stichopus japonicus, Octopus vulgaris, Perinereis nuntia, Pseudocardium sachalinense, Pseudopotamilla occelata, Reishia clavigera, Ruditapes philippinarum, Scapharca broughtonii, Todarodes pacificus, Tugali gigas
Comp. Biochem. Physiol. B
106
955-960
1993
-
-
-
-
-
-
-
-
-
-
-
-
-
43
-
-
-
-
-
31
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
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-
-
-
-
-
-
-
31
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13364
Blackstock
-
Pyruvate oxidoreductase in som ...
Capitella capitata, Glycera alba, Scolelepis fuliginosa
Biochem. Soc. Trans.
15
383-384
1987
-
-
-
-
-
-
-
4
-
-
-
-
-
3
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
6
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13365
Fields
-
Tissue-specific alanopine dehy ...
Mercenaria mercenaria
J. Exp. Mar. Biol. Ecol.
105
175-185
1987
-
-
-
-
-
-
4
11
-
-
-
1
-
1
-
-
1
-
-
2
-
-
10
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
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-
4
-
11
-
-
-
1
-
-
-
1
-
2
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13366
Manchenko
Nonfluorescent negative stain ...
Polydora ciliata, Polydora commensalis, Polydora glycymerica, Pseudopolydora paucibranchiata
Anal. Biochem.
145
308-310
1985
-
4
-
-
-
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-
-
-
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-
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-
5
-
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4
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8
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4
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8
-
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-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13368
Siegmund
Determination of meso-alanopin ...
Arenicola marina, Crassostrea angulata, Mytilus edulis, Nucula nitida
Hoppe-Seyler's Z. Physiol. Chem.
364
807-812
1983
-
-
-
-
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-
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-
4
-
-
1
-
-
6
-
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4
-
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-
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8
-
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8
-
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-
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-
-
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-
-
-
1
-
6
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13372
Plaxton
-
Purification and properties of ...
Busycotypus canaliculatum
Comp. Biochem. Physiol. B
76
321-326
1983
-
-
-
-
-
-
4
1
-
-
-
2
-
1
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-
1
-
-
3
-
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3
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6
-
-
2
-
-
-
-
-
-
2
-
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-
4
-
1
-
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-
2
-
-
-
1
-
3
-
-
3
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
13373
Storey
-
Tissue-specific alanopine dehy ...
Aphrodita aculeata
J. Exp. Zool.
225
369-378
1983
-
-
-
-
-
-
7
17
2
-
1
-
-
1
-
-
1
-
-
3
1
1
12
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
7
-
17
2
-
1
-
-
-
-
1
-
3
1
1
12
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
13371
Plaxton
-
Tissue specific isozymes of al ...
Busycotypus canaliculatum
Can. J. Zool.
60
1568-1572
1982
-
-
-
-
-
-
-
6
1
-
-
-
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1
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7
-
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2
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-
-
2
-
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2
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6
1
-
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7
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13374
Plaxton
-
Alanopine dehydrogenase: purif ...
Littorina littorea
J. Comp. Physiol.
149
57-65
1982
-
-
-
-
-
-
25
8
-
-
2
1
-
1
-
-
1
-
-
1
1
-
9
1
-
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-
-
4
-
-
2
-
-
-
-
-
-
2
-
-
-
-
25
-
8
-
-
2
1
-
-
-
1
-
1
1
-
9
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
13375
Baldwin
-
The properties and functions o ...
Bursa sp., Cantharus undosus, Conomurex luhuanus, Conus arenatus, Cymatium pileare, Cypraea tigris, Cypraecassis rufa, Lambis lambis, Lambis millepeda, Lambis scorpius, Menathais tuberosa, Mitra eremitarum, Nassarius coronatus, Nassarius glans particeps, Nerita atramentosa, Strombus aurisdianae, Strombus canarium, Strombus gibberulus, Strombus labiatus, Strombus lentiginosus, Strombus sinuatus, Tibia martinii, Xenophora crispa
Pac. Sci.
36
381-394
1982
-
-
-
-
-
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4
-
-
-
1
1
-
23
-
-
1
-
-
-
-
-
4
-
-
-
-
-
2
-
-
46
-
-
-
-
-
-
46
-
-
-
-
4
-
-
-
-
1
1
-
-
-
1
-
-
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
13369
Dando
-
Strombine [N-(carboxymethyl)-D ...
Mytilus edulis
Biochem. Soc. Trans.
9
297-298
1981
-
-
-
-
-
-
-
4
1
-
1
-
-
1
-
-
-
-
-
1
-
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6
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
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4
1
-
1
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-
1
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6
-
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-
-
2
-
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-
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-
-
-
13370
Fields
Purification and properties of ...
Crassostrea gigas
Eur. J. Biochem.
114
615-621
1981
-
-
-
-
-
-
7
10
-
-
2
-
-
2
-
-
1
-
-
2
1
-
8
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
7
-
10
-
-
2
-
-
-
-
1
-
2
1
-
8
1
-
-
-
-
-
-
-
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13367
Fields
Alanopine and strombine are no ...
Crassostrea gigas
Arch. Biochem. Biophys.
201
110-114
1980
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