BRENDA - Enzyme Database show
show all sequences of 1.5.1.17

Strombine [N-(carboxymethyl)-D-alanine]dehydrogenase and alanopine [meso-N-(1-carboxyethyl)-alanine]dehydrogenase from the mussel Mytilus edulis L.

Dando, P.R.; Biochem. Soc. Trans. 9, 297-298 (1981)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
NAD+
-
Mytilus edulis
1
-
alanopine
-
Mytilus edulis
10
-
L-Ala
-
Mytilus edulis
50
-
Gly
-
Mytilus edulis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Mytilus edulis
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38500
-
gel filtration
Mytilus edulis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mytilus edulis
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
adductor
-
Mytilus edulis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Gly + pyruvate + NADH
at 9% of the activity with L-Ala
13369
Mytilus edulis
Strombine + NAD+ + H2O
-
-
-
-
L-Ala + 2-oxobutanoate + NADH
at 14% of the activity with pyruvate
13369
Mytilus edulis
?
-
-
-
-
L-Ala + oxaloacetate + NADH
as effective as pyruvate
13369
Mytilus edulis
?
-
-
-
-
L-Ala + pyruvate + NADH
r
13369
Mytilus edulis
2,2'-Iminodipropanoate + NAD+ + H2O
-
-
-
-
L-Cys + pyruvate + NADH
at 110% of the activity with L-Ala
13369
Mytilus edulis
?
-
-
-
-
L-Ser + pyruvate + NADH
at 67% of the activity with L-Ala
13369
Mytilus edulis
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
with Ala and pyruvate as substrates
Mytilus edulis
9
-
with meso-alanopine as substrate
Mytilus edulis
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
no activity with NADP+
Mytilus edulis
NADH
no activity with NADPH
Mytilus edulis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
no activity with NADP+
Mytilus edulis
NADH
no activity with NADPH
Mytilus edulis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.03
-
NAD+
-
Mytilus edulis
1
-
alanopine
-
Mytilus edulis
10
-
L-Ala
-
Mytilus edulis
50
-
Gly
-
Mytilus edulis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Mytilus edulis
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38500
-
gel filtration
Mytilus edulis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
adductor
-
Mytilus edulis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Gly + pyruvate + NADH
at 9% of the activity with L-Ala
13369
Mytilus edulis
Strombine + NAD+ + H2O
-
-
-
-
L-Ala + 2-oxobutanoate + NADH
at 14% of the activity with pyruvate
13369
Mytilus edulis
?
-
-
-
-
L-Ala + oxaloacetate + NADH
as effective as pyruvate
13369
Mytilus edulis
?
-
-
-
-
L-Ala + pyruvate + NADH
r
13369
Mytilus edulis
2,2'-Iminodipropanoate + NAD+ + H2O
-
-
-
-
L-Cys + pyruvate + NADH
at 110% of the activity with L-Ala
13369
Mytilus edulis
?
-
-
-
-
L-Ser + pyruvate + NADH
at 67% of the activity with L-Ala
13369
Mytilus edulis
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
with Ala and pyruvate as substrates
Mytilus edulis
9
-
with meso-alanopine as substrate
Mytilus edulis
Other publictions for EC 1.5.1.17
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743404
Murphy
Changes to coral health and m ...
Montipora capitata
PeerJ
4
e1956
2016
1
1
-
-
-
-
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-
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-
1
-
2
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-
-
1
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-
1
-
1
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1
-
-
2
-
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-
1
1
-
2
-
-
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-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
741868
Lagana
-
Anaerobiosis and metabolic pl ...
Pinna nobilis
Biochem. Syst. Ecol.
56
138-143
2014
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
5
-
-
1
-
1
-
-
-
1
-
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2
-
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-
-
2
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
5
-
-
1
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
742962
Gohlke
Binding region of alanopine d ...
Arenicola marina
J. Chem. Inf. Model.
53
2493-2498
2013
-
-
-
-
-
-
1
3
-
-
-
1
-
2
-
-
1
-
-
-
-
-
3
1
-
-
-
2
-
-
-
3
1
-
-
-
-
-
3
-
-
-
-
1
1
3
-
-
-
1
-
-
-
1
-
-
-
-
3
1
-
-
-
2
-
-
-
-
-
3
3
-
-
-
13290
Carvajal
-
Lactate dehydrogenase, alanopi ...
Concholepas concholepas
Comp. Biochem. Physiol. B
108
543-550
1994
-
-
-
-
-
-
-
5
-
-
1
-
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
13292
Sato
-
Distribution of opine dehydrog ...
Anthopleura japonica, Anthopleura nigrescens, Azumapecten farreri nipponensis, Buccinum isaotakii, Cellana grata, Chlorostoma lischkei, Crassostrea gigas, Fusitriton oregonensis, Haliotis discus hannai, Heterololigo bleekeri, Liolophura japonica, Littorina brevicula, Meretrix lusoria, Mesocentrotus nudus, Mizuhopecten yessoensis, Mytilus edulis, Neptunea arthritica, no activity in Aplysia juliana, no activity in Aplysia kurodai, no activity in Asterias amurensis, no activity in Asterina pectinifera, no activity in Aurelia aurita, no activity in Balanus cariosus, no activity in Halichondria japonica, no activity in Halocynthia roretzi, no activity in Hemigrapsus sanguineus, no activity in Hexagrammos otakii, no activity in Octopus membranaceus, no activity in Oncorhynchus keta, no activity in Pagurus samuelis, no activity in Pollicipes mitella, no activity in Pugettia quadridens, no activity in Solaster paxillatus, no activity in Stichopus japonicus, Octopus vulgaris, Perinereis nuntia, Pseudocardium sachalinense, Pseudopotamilla occelata, Reishia clavigera, Ruditapes philippinarum, Scapharca broughtonii, Todarodes pacificus, Tugali gigas
Comp. Biochem. Physiol. B
106
955-960
1993
-
-
-
-
-
-
-
-
-
-
-
-
-
43
-
-
-
-
-
31
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13364
Blackstock
-
Pyruvate oxidoreductase in som ...
Capitella capitata, Glycera alba, Scolelepis fuliginosa
Biochem. Soc. Trans.
15
383-384
1987
-
-
-
-
-
-
-
4
-
-
-
-
-
3
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
6
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13365
Fields
-
Tissue-specific alanopine dehy ...
Mercenaria mercenaria
J. Exp. Mar. Biol. Ecol.
105
175-185
1987
-
-
-
-
-
-
4
11
-
-
-
1
-
1
-
-
1
-
-
2
-
-
10
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
11
-
-
-
1
-
-
-
1
-
2
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13366
Manchenko
Nonfluorescent negative stain ...
Polydora ciliata, Polydora commensalis, Polydora glycymerica, Pseudopolydora paucibranchiata
Anal. Biochem.
145
308-310
1985
-
4
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
4
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13368
Siegmund
Determination of meso-alanopin ...
Arenicola marina, Crassostrea angulata, Mytilus edulis, Nucula nitida
Hoppe-Seyler's Z. Physiol. Chem.
364
807-812
1983
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
6
-
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13372
Plaxton
-
Purification and properties of ...
Busycotypus canaliculatum
Comp. Biochem. Physiol. B
76
321-326
1983
-
-
-
-
-
-
4
1
-
-
-
2
-
1
-
-
1
-
-
3
-
-
3
-
-
-
-
-
6
-
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
1
-
-
-
2
-
-
-
1
-
3
-
-
3
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
13373
Storey
-
Tissue-specific alanopine dehy ...
Aphrodita aculeata
J. Exp. Zool.
225
369-378
1983
-
-
-
-
-
-
7
17
2
-
1
-
-
1
-
-
1
-
-
3
1
1
12
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
7
-
17
2
-
1
-
-
-
-
1
-
3
1
1
12
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
13371
Plaxton
-
Tissue specific isozymes of al ...
Busycotypus canaliculatum
Can. J. Zool.
60
1568-1572
1982
-
-
-
-
-
-
-
6
1
-
-
-
-
1
-
-
-
-
-
7
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
7
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13374
Plaxton
-
Alanopine dehydrogenase: purif ...
Littorina littorea
J. Comp. Physiol.
149
57-65
1982
-
-
-
-
-
-
25
8
-
-
2
1
-
1
-
-
1
-
-
1
1
-
9
1
-
-
-
-
4
-
-
2
-
-
-
-
-
-
2
-
-
-
-
25
-
8
-
-
2
1
-
-
-
1
-
1
1
-
9
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
13375
Baldwin
-
The properties and functions o ...
Bursa sp., Cantharus undosus, Conomurex luhuanus, Conus arenatus, Cymatium pileare, Cypraea tigris, Cypraecassis rufa, Lambis lambis, Lambis millepeda, Lambis scorpius, Menathais tuberosa, Mitra eremitarum, Nassarius coronatus, Nassarius glans particeps, Nerita atramentosa, Strombus aurisdianae, Strombus canarium, Strombus gibberulus, Strombus labiatus, Strombus lentiginosus, Strombus sinuatus, Tibia martinii, Xenophora crispa
Pac. Sci.
36
381-394
1982
-
-
-
-
-
-
4
-
-
-
1
1
-
23
-
-
1
-
-
-
-
-
4
-
-
-
-
-
2
-
-
46
-
-
-
-
-
-
46
-
-
-
-
4
-
-
-
-
1
1
-
-
-
1
-
-
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
13369
Dando
-
Strombine [N-(carboxymethyl)-D ...
Mytilus edulis
Biochem. Soc. Trans.
9
297-298
1981
-
-
-
-
-
-
-
4
1
-
1
-
-
1
-
-
-
-
-
1
-
-
6
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
4
1
-
1
-
-
-
-
-
-
1
-
-
6
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
13370
Fields
Purification and properties of ...
Crassostrea gigas
Eur. J. Biochem.
114
615-621
1981
-
-
-
-
-
-
7
10
-
-
2
-
-
2
-
-
1
-
-
2
1
-
8
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
7
-
10
-
-
2
-
-
-
-
1
-
2
1
-
8
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13367
Fields
Alanopine and strombine are no ...
Crassostrea gigas
Arch. Biochem. Biophys.
201
110-114
1980
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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-
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-
-
1
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2
-
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-
-
-
-
2
-
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-
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2
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-
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-
1
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-
1
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2
-
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-
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-
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-