BRENDA - Enzyme Database
show all sequences of 1.5.1.17

Tissue-specific alanopine dehydrogenase from the gill and strombine dehydrogenase from the foot muscle of the cherrystone clam Mercenaria mercenaria

Fields, J.H.A.; Storey, K.B.; J. Exp. Mar. Biol. Ecol. 105, 175-185 (1987)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
D-lactate
weak
Mercenaria mercenaria
L-lactate
weak
Mercenaria mercenaria
meso-Alanopine
weak
Mercenaria mercenaria
succinate
weak
Mercenaria mercenaria
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.38
-
pyruvate
-
Mercenaria mercenaria
2.5
-
2-oxobutyrate
-
Mercenaria mercenaria
11.9
-
glyoxylate
-
Mercenaria mercenaria
15
-
L-2-aminobutyrate
-
Mercenaria mercenaria
15.1
-
L-Ala
-
Mercenaria mercenaria
20
-
L-Val
-
Mercenaria mercenaria
23
-
L-Cys
-
Mercenaria mercenaria
28
-
L-Ala
-
Mercenaria mercenaria
110
-
L-Thr
-
Mercenaria mercenaria
247
-
L-Ser
-
Mercenaria mercenaria
291
-
Gly
-
Mercenaria mercenaria
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Mercenaria mercenaria
the enzyme functions as the terminal dehydrogenase of glycolysis and has a role in maintaining energy production under the stresses of environmental or functional anoxia
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Mercenaria mercenaria
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Mercenaria mercenaria
Source Tissue
Source Tissue
Commentary
Organism
Textmining
gill
-
Mercenaria mercenaria
-
mantle
-
Mercenaria mercenaria
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
Gly + pyruvate + NADH
at 69% of the activity with L-Ala
13365
Mercenaria mercenaria
Strombine + NAD+ + H2O
-
-
-
-
L-2-Aminobutyrate + pyruvate + NADH
at 94% of the activity with L-Ala
13365
Mercenaria mercenaria
?
-
-
-
-
L-Ala + 2-oxobutanoate + NADH
at 22% the activity with pyruvate
13365
Mercenaria mercenaria
?
-
-
-
-
L-Ala + glyoxylate + NADH
at 44% the activity with pyruvate
13365
Mercenaria mercenaria
?
-
-
-
-
L-Ala + pyruvate + NADH
-
13365
Mercenaria mercenaria
2,2'-Iminodipropanoate + NAD+ + H2O
-
-
-
-
L-Cys + pyruvate + NADH
at 90% of the activity with L-Ala
13365
Mercenaria mercenaria
?
-
-
-
-
L-Ser + pyruvate + NADH
at 90% of the activity with L-Ala
13365
Mercenaria mercenaria
?
-
-
-
-
L-Thr + pyruvate + NADH
at 62% of the activity with L-Ala
13365
Mercenaria mercenaria
?
-
-
-
-
L-Val + pyruvate + NADH
at 65% of the activity with L-Ala
13365
Mercenaria mercenaria
?
-
-
-
-
additional information
the enzyme functions as the terminal dehydrogenase of glycolysis and has a role in maintaining energy production under the stresses of environmental or functional anoxia
13365
Mercenaria mercenaria
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Mercenaria mercenaria
NADH
-
Mercenaria mercenaria
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Mercenaria mercenaria
NADH
-
Mercenaria mercenaria
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-lactate
weak
Mercenaria mercenaria
L-lactate
weak
Mercenaria mercenaria
meso-Alanopine
weak
Mercenaria mercenaria
succinate
weak
Mercenaria mercenaria
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.38
-
pyruvate
-
Mercenaria mercenaria
2.5
-
2-oxobutyrate
-
Mercenaria mercenaria
11.9
-
glyoxylate
-
Mercenaria mercenaria
15
-
L-2-aminobutyrate
-
Mercenaria mercenaria
15.1
-
L-Ala
-
Mercenaria mercenaria
20
-
L-Val
-
Mercenaria mercenaria
23
-
L-Cys
-
Mercenaria mercenaria
28
-
L-Ala
-
Mercenaria mercenaria
110
-
L-Thr
-
Mercenaria mercenaria
247
-
L-Ser
-
Mercenaria mercenaria
291
-
Gly
-
Mercenaria mercenaria
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
additional information
Mercenaria mercenaria
the enzyme functions as the terminal dehydrogenase of glycolysis and has a role in maintaining energy production under the stresses of environmental or functional anoxia
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Mercenaria mercenaria
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
gill
-
Mercenaria mercenaria
-
mantle
-
Mercenaria mercenaria
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
Gly + pyruvate + NADH
at 69% of the activity with L-Ala
13365
Mercenaria mercenaria
Strombine + NAD+ + H2O
-
-
-
-
L-2-Aminobutyrate + pyruvate + NADH
at 94% of the activity with L-Ala
13365
Mercenaria mercenaria
?
-
-
-
-
L-Ala + 2-oxobutanoate + NADH
at 22% the activity with pyruvate
13365
Mercenaria mercenaria
?
-
-
-
-
L-Ala + glyoxylate + NADH
at 44% the activity with pyruvate
13365
Mercenaria mercenaria
?
-
-
-
-
L-Ala + pyruvate + NADH
-
13365
Mercenaria mercenaria
2,2'-Iminodipropanoate + NAD+ + H2O
-
-
-
-
L-Cys + pyruvate + NADH
at 90% of the activity with L-Ala
13365
Mercenaria mercenaria
?
-
-
-
-
L-Ser + pyruvate + NADH
at 90% of the activity with L-Ala
13365
Mercenaria mercenaria
?
-
-
-
-
L-Thr + pyruvate + NADH
at 62% of the activity with L-Ala
13365
Mercenaria mercenaria
?
-
-
-
-
L-Val + pyruvate + NADH
at 65% of the activity with L-Ala
13365
Mercenaria mercenaria
?
-
-
-
-
additional information
the enzyme functions as the terminal dehydrogenase of glycolysis and has a role in maintaining energy production under the stresses of environmental or functional anoxia
13365
Mercenaria mercenaria
?
-
-
-
-
Other publictions for EC 1.5.1.17
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743404
Murphy
Changes to coral health and m ...
Montipora capitata
PeerJ
4
e1956
2016
1
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
1
1
-
-
-
1
-
-
2
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
741868
Lagana
-
Anaerobiosis and metabolic pl ...
Pinna nobilis
Biochem. Syst. Ecol.
56
138-143
2014
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
5
-
-
1
-
1
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
5
-
-
1
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
742962
Gohlke
Binding region of alanopine d ...
Arenicola marina
J. Chem. Inf. Model.
53
2493-2498
2013
-
-
-
-
-
-
1
3
-
-
-
1
-
2
-
-
1
-
-
-
-
-
3
1
2
-
-
-
2
-
-
-
3
1
-
-
-
-
-
3
-
-
-
-
1
1
3
-
-
-
1
-
-
-
1
-
-
-
-
3
1
-
-
-
2
-
-
-
-
-
3
3
-
-
-
13290
Carvajal
-
Lactate dehydrogenase, alanopi ...
Concholepas concholepas
Comp. Biochem. Physiol. B
108
543-550
1994
-
-
-
-
-
-
-
5
-
-
1
-
-
1
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
1
-
-
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-
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-
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-
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5
-
-
1
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
13292
Sato
-
Distribution of opine dehydrog ...
Anthopleura japonica, Anthopleura nigrescens, Azumapecten farreri nipponensis, Buccinum isaotakii, Cellana grata, Chlorostoma lischkei, Crassostrea gigas, Fusitriton oregonensis, Haliotis discus hannai, Heterololigo bleekeri, Liolophura japonica, Littorina brevicula, Meretrix lusoria, Mesocentrotus nudus, Mizuhopecten yessoensis, Mytilus edulis, Neptunea arthritica, no activity in Aplysia juliana, no activity in Aplysia kurodai, no activity in Asterias amurensis, no activity in Asterina pectinifera, no activity in Aurelia aurita, no activity in Balanus cariosus, no activity in Halichondria japonica, no activity in Halocynthia roretzi, no activity in Hemigrapsus sanguineus, no activity in Hexagrammos otakii, no activity in Octopus membranaceus, no activity in Oncorhynchus keta, no activity in Pagurus samuelis, no activity in Pollicipes mitella, no activity in Pugettia quadridens, no activity in Solaster paxillatus, no activity in Stichopus japonicus, Octopus vulgaris, Perinereis nuntia, Pseudocardium sachalinense, Pseudopotamilla occelata, Reishia clavigera, Ruditapes philippinarum, Scapharca broughtonii, Todarodes pacificus, Tugali gigas
Comp. Biochem. Physiol. B
106
955-960
1993
-
-
-
-
-
-
-
-
-
-
-
-
-
43
-
-
-
-
-
31
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13364
Blackstock
-
Pyruvate oxidoreductase in som ...
Capitella capitata, Glycera alba, Scolelepis fuliginosa
Biochem. Soc. Trans.
15
383-384
1987
-
-
-
-
-
-
-
4
-
-
-
-
-
3
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
6
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
2
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13365
Fields
-
Tissue-specific alanopine dehy ...
Mercenaria mercenaria
J. Exp. Mar. Biol. Ecol.
105
175-185
1987
-
-
-
-
-
-
4
11
-
-
-
1
-
1
-
-
1
-
-
2
-
-
10
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
11
-
-
-
1
-
-
-
1
-
2
-
-
10
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13366
Manchenko
Nonfluorescent negative stain ...
Polydora ciliata, Polydora commensalis, Polydora glycymerica, Pseudopolydora paucibranchiata
Anal. Biochem.
145
308-310
1985
-
4
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
8
-
-
-
-
4
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13368
Siegmund
Determination of meso-alanopin ...
Arenicola marina, Crassostrea angulata, Mytilus edulis, Nucula nitida
Hoppe-Seyler's Z. Physiol. Chem.
364
807-812
1983
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
6
-
-
4
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
6
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13372
Plaxton
-
Purification and properties of ...
Busycotypus canaliculatum
Comp. Biochem. Physiol. B
76
321-326
1983
-
-
-
-
-
-
4
1
-
-
-
2
-
1
-
-
1
-
-
3
-
-
3
-
-
-
-
-
-
6
-
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
1
-
-
-
2
-
-
-
1
-
3
-
-
3
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
13373
Storey
-
Tissue-specific alanopine dehy ...
Aphrodita aculeata
J. Exp. Zool.
225
369-378
1983
-
-
-
-
-
-
7
17
2
-
1
-
-
1
-
-
1
-
-
3
1
1
12
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
7
-
17
2
-
1
-
-
-
-
1
-
3
1
1
12
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
13371
Plaxton
-
Tissue specific isozymes of al ...
Busycotypus canaliculatum
Can. J. Zool.
60
1568-1572
1982
-
-
-
-
-
-
-
6
1
-
-
-
-
1
-
-
-
-
-
7
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
7
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13374
Plaxton
-
Alanopine dehydrogenase: purif ...
Littorina littorea
J. Comp. Physiol.
149
57-65
1982
-
-
-
-
-
-
25
8
-
-
2
1
-
1
-
-
1
-
-
1
1
-
9
1
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
2
-
-
-
-
25
-
8
-
-
2
1
-
-
-
1
-
1
1
-
9
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
13375
Baldwin
-
The properties and functions o ...
Bursa sp., Cantharus undosus, Conomurex luhuanus, Conus arenatus, Cymatium pileare, Cypraea tigris, Cypraecassis rufa, Lambis lambis, Lambis millepeda, Lambis scorpius, Menathais tuberosa, Mitra eremitarum, Nassarius coronatus, Nassarius glans particeps, Nerita atramentosa, Strombus aurisdianae, Strombus canarium, Strombus gibberulus, Strombus labiatus, Strombus lentiginosus, Strombus sinuatus, Tibia martinii, Xenophora crispa
Pac. Sci.
36
381-394
1982
-
-
-
-
-
-
4
-
-
-
1
1
-
23
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
2
-
-
46
-
-
-
-
-
-
46
-
-
-
-
4
-
-
-
-
1
1
-
-
-
1
-
-
-
-
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
13369
Dando
-
Strombine [N-(carboxymethyl)-D ...
Mytilus edulis
Biochem. Soc. Trans.
9
297-298
1981
-
-
-
-
-
-
-
4
1
-
1
-
-
1
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
4
1
-
1
-
-
-
-
-
-
1
-
-
6
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
13370
Fields
Purification and properties of ...
Crassostrea gigas
Eur. J. Biochem.
114
615-621
1981
-
-
-
-
-
-
7
10
-
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2
-
-
2
-
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1
-
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2
1
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8
1
-
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-
-
-
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-
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2
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2
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7
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10
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2
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1
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2
1
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8
1
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13367
Fields
Alanopine and strombine are no ...
Crassostrea gigas
Arch. Biochem. Biophys.
201
110-114
1980
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1
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