Literature summary for 1.5.1.10 extracted from

Johansson, E.; Steffens, J.J.; Emptage, M.; Lindqvist, Y.; Schneider, G.
Cloning, expression, purification and crystallization of saccharopine reductase from Magnaporthe grisea (2000), Acta Crystallogr. Sect. D, 56, 662-664.

No PubMed abstract available

Search for more literature for 1.5.1.10

Cloned(Commentary)

Cloned (Comment)	Organism
cloned in Escherichia coli	Pyricularia grisea

Crystallization (Commentary)

Crystallization (Comment)	Organism
with hanging-drop vapour-diffusion technique	Pyricularia grisea

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
48900	-	alpha2, 2 * 48900, predicted mass from the gene sequence	Pyricularia grisea
50000	-	alpha2, 2 * 50000, SDS-PAGE	Pyricularia grisea
84000	-	gel filtration with Superdex 200	Pyricularia grisea

Organism

Organism	UniProt	Comment	Textmining
Pyricularia grisea	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
purification of heterologously expressed enzyme	Pyricularia grisea

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-aminoadipate 6-semialdehyde + L-glutamate + NADPH	-	Pyricularia grisea	N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O	-	r

Subunits

Subunits	Comment	Organism
dimer	alpha2, 2 * 50000, SDS-PAGE	Pyricularia grisea
dimer	alpha2, 2 * 48900, predicted mass from the gene sequence	Pyricularia grisea

Cofactor

Cofactor	Comment	Organism	Structure
NADPH	-	Pyricularia grisea

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Release 2023.1 (January 2023)