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Literature summary for 1.4.3.4 extracted from

  • Cui, Z.; Zhang, Y.; Inoue, H.; Yogo, S.; Hirasawa, E.
    Purification and molecular analysis of a monoamine oxidase isolated from Narcissus tazetta (2013), Biosci. Biotechnol. Biochem., 77, 1728-1733.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
2,2'-bipyridyl 50% inhibition at 1 mM Narcissus tazetta
2-Bromoethylamine complete inhibition at 0.01 mM Narcissus tazetta
additional information hardly inhibited by 1 mM o-phenanthroline (4%), pargyline (2%) and clorgyline (1%) Narcissus tazetta
Semicarbazide complete inhibition at 0.01 mM Narcissus tazetta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.059
-
n-Propylamine at pH 7.0 and 37°C Narcissus tazetta
0.11
-
methylamine at pH 7.0 and 37°C Narcissus tazetta
0.11
-
n-butylamine at pH 7.0 and 37°C Narcissus tazetta
0.13
-
ethylamine at pH 7.0 and 37°C Narcissus tazetta
0.41
-
benzylamine at pH 7.0 and 37°C Narcissus tazetta
0.5
-
n-Pentylamine at pH 7.0 and 37°C Narcissus tazetta
1.4
-
2-Phenylethylamine at pH 7.0 and 37°C Narcissus tazetta
2
-
ethanolamine at pH 7.0 and 37°C Narcissus tazetta

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ the enzyme contains 2.44 mol of copper atoms per mole of enzyme Narcissus tazetta
Mn2+ 133% activity at 0.1 mM Mn2+ Narcissus tazetta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
1 * 75000 + 1 * 65000, calculated from amino acid sequence Narcissus tazetta
75000
-
1 * 75000 + 1 * 65000, calculated from amino acid sequence Narcissus tazetta
135000
-
gel filtration Narcissus tazetta

Organism

Organism UniProt Comment Textmining
Narcissus tazetta
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Sephadex G-25 gel filtration, DEAE Sephacel gel filtration, and hydroxyapatite column chromatography Narcissus tazetta

Source Tissue

Source Tissue Comment Organism Textmining
shoot
-
Narcissus tazetta
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0032
-
crude extract, at pH 7.0 and 37°C Narcissus tazetta
1.365
-
1544fold purified enzyme, at pH 7.0 and 37°C Narcissus tazetta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phenylethylamine + H2O + O2 9% activity compared to n-propylamine Narcissus tazetta 2-phenylethanal + NH3 + H2O2
-
?
benzylamine + H2O + O2 74% activity compared to n-propylamine Narcissus tazetta benzaldehyde + NH3 + H2O2
-
?
ethanolamine + H2O + O2 50% activity compared to n-propylamine Narcissus tazetta glycolaldehyde + NH3 + H2O2
-
?
ethylamine + H2O + O2 84% activity compared to n-propylamine Narcissus tazetta ethanal + NH3 + H2O2
-
?
hexylamine + H2O + O2 7% activity compared to n-propylamine Narcissus tazetta hexanal + NH3 + H2O2
-
?
methylamine + H2O + O2 34% activity compared to n-propylamine Narcissus tazetta formaldehyde + NH3 + H2O2
-
?
additional information no activity with tryptamine, serotonin, dopamine, tyramine, histamine, putrescine, cadaverine, diaminodecane, spermidine, and spermine Narcissus tazetta ?
-
?
n-butylamine + H2O + O2 98% activity compared to n-propylamine Narcissus tazetta butyraldehyde + NH3 + H2O2
-
?
n-pentylamine + H2O + O2 56% activity compared to n-propylamine Narcissus tazetta pentaldehyde + NH3 + H2O2
-
?
n-propylamine + H2O + O2 100% activity Narcissus tazetta propionaldehyde + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
heterodimer 1 * 75000 + 1 * 65000, calculated from amino acid sequence Narcissus tazetta

Synonyms

Synonyms Comment Organism
MAO
-
Narcissus tazetta

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 80 the purified enzyme retains its activity at temperatures up to 70°C but loses activity quickly after pre-incubation at temperatures of 75°C (less than 50%) and higher Narcissus tazetta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Narcissus tazetta

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
Narcissus tazetta

Cofactor

Cofactor Comment Organism Structure
FAD
-
Narcissus tazetta

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1
-
at pH 7.0 and 37°C Narcissus tazetta 2,2'-bipyridyl