Application | Comment | Organism |
---|---|---|
synthesis | use of mutant Y228L/R283G for the deracemization of racemic(RS)-1-phenylethan-1-amine with NaBH4 to produce (1S)-1-phenylethan-1-amine with an enantiomeric excess of 99% | Sus scrofa |
Crystallization (Comment) | Organism |
---|---|
structure of mutant Y228L/R283G in complex with (R)-1-phenylethan-1-amine. The phenyl ring is flipped to the xylene ring of the isoalloxadine ring of FAD. The side chain of Phe242 rotates approximately 60 degrees between the wild-type and variant and forms part of the active site wall, while the phenyl ring of (R)-1-phenylethan-1-amine forms a hydrophobic interaction along with this rotation | Sus scrofa |
Protein Variants | Comment | Organism |
---|---|---|
Y228L/F242I/R283G | mutant is able to oxidize 1-(2-naphthyl)ethylamine | Sus scrofa |
Y228L/R283G | mutant displays altered substrate specificity toward (R)-amines acting on alpha-methylbenzylamine and its derivatives, alpha-ethylbenzylamine, alkylamine, and cyclic secondary amines, and totally losing the activities toward the original substrates, D-amino acids | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | P00371 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.5 | - |
mutant Y228L/R283G, substrate (1R)-1-phenylethan-1-amine, pH not specified in the publication, temperature not specified in the publication | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1R)-1-(2-naphthyl)ethylamine + H2O + O2 | substrate of mutant Y228L/F242I/R283G | Sus scrofa | 1-(naphthalen-2-yl)ethan-1-one + NH3 + H2O2 | - |
? | |
(1R)-1-phenylethan-1-amine + H2O + O2 | no substrate of wild-type, but mutant Y228L/R283G | Sus scrofa | 1-phenylethan-1-one + NH3 + H2O2 | - |
? | |
(1R)-1-phenylpropan-1-amine + H2O + O2 | mutant Y228L/R283G, 109% of the activity with (1R)-1-phenylethan-1-amine | Sus scrofa | 1-phenylpropan-1-one + NH3 + H2O2 | - |
? | |
1-(4-fluorophenyl)ethan-1-amine + H2O + O2 | mutant Y228L/R283G, 22.7% of the activity with (1R)-1-phenylethan-1-amine | Sus scrofa | 1-phenylethan-1-one + NH3 + H2O2 | - |
? |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.83 | - |
(1R)-1-(2-naphthyl)ethylamine | mutant Y228L/F242I/R283G, pH not specified in the publication, temperature not specified in the publication | Sus scrofa |