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Literature summary for 1.4.3.22 extracted from

  • Yang, R.; Chen, H.; Han, Y.; Gu, Z.
    Purification of diamine oxidase and its properties in germinated fava bean (Vicia faba L.) (2012), J. Sci. Food Agric., 92, 1709-1715.
    View publication on PubMed
Search for more literature for 1.4.3.22

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 2.94% residual activity at 0.5 mM Vicia faba
aminoguanidine 3.92% residual activity at 0.25 mM Vicia faba
Cu2+ 7.84% residual activity at 3 mM Vicia faba
EDTA-Na2 76.47% residual activity at 1 mM Vicia faba
EGTA 71.57% residual activity at 1 mM Vicia faba
Fe3+ 10.78% residual activity at 3 mM Vicia faba
L-cysteine complete inhibition at 0.1 mM Vicia faba
Mg2+ 78.43% residual activity at 3 mM Vicia faba

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
 putrescine at pH 6.5 and 40°C Vicia faba
0.96
-
 spermidine at pH 6.5 and 40°C Vicia faba

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not influenced by Ca2+, Zn2+, Mn2+, and Ba2+ Vicia faba

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 2 * 52000, SDS-PAGE Vicia faba
121000
-
 gel filtration Vicia faba

Organism

Organism UniProt Comment Textmining
Vicia faba
-
 cultivar Qidou 2
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephadex G-100 gel filtration Vicia faba

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Vicia faba
-
shoot
-
 Vicia faba
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 the crude extract has a specific activity of 9.1 units/mg, while the 215fold purified enzyme has a specific activity of 1962.47 units/mg. A change in absorbance at 555 nm of 0.01 per minute is considered as one unit of enzyme activity Vicia faba

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme does not degrade spermine Vicia faba ?
-
 ?
putrescine + H2O + O2
-
 Vicia faba ?
-
 ?
spermidine + H2O + O2
-
 Vicia faba ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 52000, SDS-PAGE Vicia faba

Synonyms

Synonyms Comment Organism
DAO
-
 Vicia faba
EC 1.4.3.6 formerly Vicia faba

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Vicia faba

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 the enzyme retains its activity after being incubated at 30°C for 30 min, the enzyme is rapidly deactivated above 45°C and reaches almost zero activity at 60°C Vicia faba

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Vicia faba

Expression

Organism Comment Expression
Vicia faba enzyme activity in the shoot is undetectable up to 2 days of germination but then increases significantly up

General Information

General Information Comment Organism
metabolism diamine oxidase is one of the key enzymes for gamma-aminobutyric acid formation Vicia faba