Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
115000 | 116000 | gel filtration | Trichoderma cf. aureoviride |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + O2 + H2O | Trichoderma cf. aureoviride | 8.3% activity compared to L-lysine | 5-carbamidamido-2-oxopentanoate + NH3 + H2O2 | - |
? | |
L-arginine + O2 + H2O | Trichoderma cf. aureoviride Rifai VKM F-4268D | 8.3% activity compared to L-lysine | 5-carbamidamido-2-oxopentanoate + NH3 + H2O2 | - |
? | |
L-lysine + O2 + H2O | Trichoderma cf. aureoviride | the stereospecific enzyme oxidizes almost exclusively L-lysine | 6-amino-2-oxohexanoate + NH3 + H2O2 | - |
? | |
L-lysine + O2 + H2O | Trichoderma cf. aureoviride Rifai VKM F-4268D | the stereospecific enzyme oxidizes almost exclusively L-lysine | 6-amino-2-oxohexanoate + NH3 + H2O2 | - |
? | |
L-ornithine + O2 + H2O | Trichoderma cf. aureoviride | 5.8% activity compared to L-lysine | 5-amino-2-oxopentanoate + NH3 + H2O2 | - |
? | |
L-ornithine + O2 + H2O | Trichoderma cf. aureoviride Rifai VKM F-4268D | 5.8% activity compared to L-lysine | 5-amino-2-oxopentanoate + NH3 + H2O2 | - |
? | |
additional information | Trichoderma cf. aureoviride | no activity with The L-isomers of leucine, phenylalanine, citrulline, alanine, asparagine, histidine, ornithine, glutamine, threonine, tyrosine, isoleucine, valine, as well as glycine and D-lysine | ? | - |
? | |
additional information | Trichoderma cf. aureoviride Rifai VKM F-4268D | no activity with The L-isomers of leucine, phenylalanine, citrulline, alanine, asparagine, histidine, ornithine, glutamine, threonine, tyrosine, isoleucine, valine, as well as glycine and D-lysine | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma cf. aureoviride | - |
- |
- |
Trichoderma cf. aureoviride Rifai VKM F-4268D | - |
- |
- |
Purification (Comment) | Organism |
---|---|
octyl Sepharose column chromatography, DEAE ToyoPearl column chromatography, and gel filtration | Trichoderma cf. aureoviride |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Trichoderma cf. aureoviride | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.29 | - |
culture liquid, at pH 8.0 and 25°C | Trichoderma cf. aureoviride |
90 | - |
after 310fold purification, at pH 8.0 and 25°C | Trichoderma cf. aureoviride |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + O2 + H2O | 8.3% activity compared to L-lysine | Trichoderma cf. aureoviride | 5-carbamidamido-2-oxopentanoate + NH3 + H2O2 | - |
? | |
L-arginine + O2 + H2O | 8.3% activity compared to L-lysine | Trichoderma cf. aureoviride Rifai VKM F-4268D | 5-carbamidamido-2-oxopentanoate + NH3 + H2O2 | - |
? | |
L-lysine + O2 + H2O | the stereospecific enzyme oxidizes almost exclusively L-lysine | Trichoderma cf. aureoviride | 6-amino-2-oxohexanoate + NH3 + H2O2 | - |
? | |
L-lysine + O2 + H2O | the stereospecific enzyme oxidizes almost exclusively L-lysine | Trichoderma cf. aureoviride Rifai VKM F-4268D | 6-amino-2-oxohexanoate + NH3 + H2O2 | - |
? | |
L-ornithine + O2 + H2O | 5.8% activity compared to L-lysine | Trichoderma cf. aureoviride | 5-amino-2-oxopentanoate + NH3 + H2O2 | - |
? | |
L-ornithine + O2 + H2O | 5.8% activity compared to L-lysine | Trichoderma cf. aureoviride Rifai VKM F-4268D | 5-amino-2-oxopentanoate + NH3 + H2O2 | - |
? | |
additional information | no activity with The L-isomers of leucine, phenylalanine, citrulline, alanine, asparagine, histidine, ornithine, glutamine, threonine, tyrosine, isoleucine, valine, as well as glycine and D-lysine | Trichoderma cf. aureoviride | ? | - |
? | |
additional information | no activity with The L-isomers of leucine, phenylalanine, citrulline, alanine, asparagine, histidine, ornithine, glutamine, threonine, tyrosine, isoleucine, valine, as well as glycine and D-lysine | Trichoderma cf. aureoviride Rifai VKM F-4268D | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 57000-58000, SDS-PAGE | Trichoderma cf. aureoviride |
Synonyms | Comment | Organism |
---|---|---|
L-lysine-alpha-oxidase | - |
Trichoderma cf. aureoviride |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the enzyme is stable at temperatures up to 50°C | Trichoderma cf. aureoviride |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.2 | - |
Trichoderma cf. aureoviride |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | about 50% activity at pH 6.0, about 80% activity at pH 7.0, 100% activity at pH 8.0, about 85% activity at pH 9.0, about 78% activity at pH 9.5 | Trichoderma cf. aureoviride |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Trichoderma cf. aureoviride |
Organism | Comment | Expression |
---|---|---|
Trichoderma cf. aureoviride | enzyme expression is induced in submerged culture on wheat bran under salt stress conditions (6% (w/v) NaCl) | up |