Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudoalteromonas sp. ANT 178 |
Crystallization (Comment) | Organism |
---|---|
homology modeling of structure. Enzyme exhibits several cold-adapted features | Pseudoalteromonas sp. ANT 178 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 1 mM, 8% residual activity | Pseudoalteromonas sp. ANT 178 | |
Cr2+ | 1 mM, 13% residual activity | Pseudoalteromonas sp. ANT 178 | |
Cu2+ | 1 mM, 61% residual activity | Pseudoalteromonas sp. ANT 178 | |
EDTA | 1 mM, 92% residual activity | Pseudoalteromonas sp. ANT 178 | |
Fe2+ | 1 mM, 75% residual activity | Pseudoalteromonas sp. ANT 178 | |
Hg2+ | 1 mM, 29% residual activity | Pseudoalteromonas sp. ANT 178 | |
Pb2+ | 1 mM, no residual activity | Pseudoalteromonas sp. ANT 178 | |
Thiourea | 1 mM, 52% residual activity | Pseudoalteromonas sp. ANT 178 | |
Zn2+ | 1 mM, 80% residual activity | Pseudoalteromonas sp. ANT 178 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
L-leucine | pH 9, 30°C | Pseudoalteromonas sp. ANT 178 | |
0.48 | - |
L-tyrosine | pH 9, 30°C | Pseudoalteromonas sp. ANT 178 | |
0.64 | - |
L-proline | pH 9, 30°C | Pseudoalteromonas sp. ANT 178 | |
0.75 | - |
DL-methionine | pH 9, 30°C | Pseudoalteromonas sp. ANT 178 | |
0.84 | - |
L-arginine | pH 9, 30°C | Pseudoalteromonas sp. ANT 178 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | 2 M NaCl, 140% of initial activity | Pseudoalteromonas sp. ANT 178 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas sp. ANT 178 | A0A385GJJ6 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
275 | - |
pH 9, 30°C, substrate L-leucine | Pseudoalteromonas sp. ANT 178 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-methionine + H2O + NAD+ | - |
Pseudoalteromonas sp. ANT 178 | 3-methylthio-2-oxobutanoate + NH3 + NADH + H+ | - |
? | |
L-arginine + H2O + NAD+ | - |
Pseudoalteromonas sp. ANT 178 | 5-[(diaminomethyl)amino]-2-oxopentanoic acid + NH3 + NADH + H+ | - |
? | |
L-leucine + H2O + NAD+ | - |
Pseudoalteromonas sp. ANT 178 | 4-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
? | |
L-proline + H2O + NAD+ | - |
Pseudoalteromonas sp. ANT 178 | ? + NH3 + NADH + H+ | - |
? | |
L-tyrosine + H2O + NAD+ | - |
Pseudoalteromonas sp. ANT 178 | ? + NH3 + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42600, calculated from sequence, x * 44000, SDS-PAGE, recombinant protein | Pseudoalteromonas sp. ANT 178 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Pseudoalteromonas sp. ANT 178 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
120 min, 85% residual activity | Pseudoalteromonas sp. ANT 178 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.1 | - |
L-arginine | pH 9, 30°C | Pseudoalteromonas sp. ANT 178 | |
16.6 | - |
DL-methionine | pH 9, 30°C | Pseudoalteromonas sp. ANT 178 | |
20.8 | - |
L-proline | pH 9, 30°C | Pseudoalteromonas sp. ANT 178 | |
26.4 | - |
L-tyrosine | pH 9, 30°C | Pseudoalteromonas sp. ANT 178 | |
30.13 | - |
L-leucine | pH 9, 30°C | Pseudoalteromonas sp. ANT 178 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pseudoalteromonas sp. ANT 178 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.8 | - |
L-arginine | pH 9, 30°C | Pseudoalteromonas sp. ANT 178 | |
22.1 | - |
DL-methionine | pH 9, 30°C | Pseudoalteromonas sp. ANT 178 | |
32.5 | - |
L-proline | pH 9, 30°C | Pseudoalteromonas sp. ANT 178 | |
55 | - |
L-tyrosine | pH 9, 30°C | Pseudoalteromonas sp. ANT 178 | |
91.3 | - |
L-leucine | pH 9, 30°C | Pseudoalteromonas sp. ANT 178 |