Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibitory: EDTA, Ca2+, Mg2+ | Citrobacter freundii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
L-leucine | pH not specified in the publication, temperature not specified in the publication | Citrobacter freundii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
320000 | - |
gel filtration | Citrobacter freundii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter freundii | - |
- |
- |
Citrobacter freundii JK-91 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Citrobacter freundii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
320 | - |
pH not specified in the publication, temperature not specified in the publication | Citrobacter freundii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucine + H2O + NAD+ | - |
Citrobacter freundii | 4-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
? | |
L-leucine + H2O + NAD+ | - |
Citrobacter freundii JK-91 | 4-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
? | |
additional information | no substrates: D-leucine, L-arginine, aspartate, glycine, L-threonine | Citrobacter freundii | ? | - |
? | |
additional information | no substrates: D-leucine, L-arginine, aspartate, glycine, L-threonine | Citrobacter freundii JK-91 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 40000, SDS-PAGE | Citrobacter freundii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Citrobacter freundii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 75 | - |
Citrobacter freundii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.5 | - |
- |
Citrobacter freundii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9 | 11 | - |
Citrobacter freundii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no cofactor: NADP+, NADPH | Citrobacter freundii | |
NAD+ | - |
Citrobacter freundii | |
NADH | - |
Citrobacter freundii |