Application | Comment | Organism |
---|---|---|
biotechnology | an efficient stereospecific enzymatic synthesis of L-valine, L-leucine, L-norvaline, L-norleucine and L-isoleucine from the corresponding alpha-keto acids by coupling the reactions catalysed by leucine dehydrogenase and glucose dehydrogenase/galactose mutarotase. Giving high yields of L-amino acids, the procedure is economical and easy to perform and to monitor at a synthetically useful scale (1-10 g) | Geobacillus stearothermophilus |
Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | P13154 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-keto beta-methylvalerate + NH3 + NADH + H+ | yield: 95% | Geobacillus stearothermophilus | L-isoleucine + H2O + NAD+ | - |
? | |
alpha-ketocaproate + NH3 + NADH + H+ | yield: 80% | Geobacillus stearothermophilus | L-norleucine + H2O + NAD+ | - |
? | |
alpha-ketoisocaproate + NH3 + NADH + H+ | yield: 92.5% | Geobacillus stearothermophilus | L-leucine + H2O + NAD+ | - |
? | |
alpha-ketoisovalerate + NH3 + NADH + H+ | yield: 90% | Geobacillus stearothermophilus | L-valine + H2O + NAD+ | - |
? | |
alpha-ketovalerate + NH3 + NADH + H+ | yield: 92% | Geobacillus stearothermophilus | L-norvaline + H2O + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LeuDH | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Geobacillus stearothermophilus |