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Literature summary for 1.4.1.9 extracted from

  • Nagata, S.; Bakthavatsalam, S.; Galkin, A.G.; Asada, H.; Sakai, S.; Esaki, N.; Soda, K.; Ohshima, T.; Nagasaki, S.; Misono, H.
    Gene cloning, purification, and characterization of thermostable and halophilic leucine dehydrogenase from a halophilic thermophile, Bacillus licheniformis TSN9 (1995), Appl. Microbiol. Biotechnol., 44, 432-438.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned into Escherichia coli JM 109 with a vector plasmid pUC18 Bacillus licheniformis

Inhibitors

Inhibitors Comment Organism Structure
NADH noncompetitive inhibition with respect to L-Leu Bacillus licheniformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
4-methyl-2-oxopentanoate
-
Bacillus licheniformis
2.1
-
L-Leu
-
Bacillus licheniformis
2.4
-
2-oxopentanoate
-
Bacillus licheniformis
3.3
-
L-Ile
-
Bacillus licheniformis
3.8
-
3-methyl-2-oxobutanoate
-
Bacillus licheniformis
12.5
-
L-Val
-
Bacillus licheniformis
330
-
NH3
-
Bacillus licheniformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
8 * 44000, SDS-PAGE Bacillus licheniformis
360000
-
gel filtration Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxopentanoate + NH3 + NADH + H+
-
Bacillus licheniformis L-norvaline + H2O + NAD+
-
r
L-2-aminobutanoate + H2O + NAD+ 32% of the activity with L-Leu Bacillus licheniformis 2-oxobutanoate + NH3 + NADH + H+
-
r
L-Ala + H2O + NAD+ 10% of the activity with L-Leu Bacillus licheniformis 2-oxopropanoate + NH3 + NADH
-
?
L-Ile + H2O + NAD+ 72% of the activity with L-Leu Bacillus licheniformis 3-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Leu + H2O + NAD+
-
Bacillus licheniformis 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-norleucine + H2O + NAD+ 7% of the activity with L-Leu Bacillus licheniformis 2-oxohexanoate + NH3 + NADH
-
r
L-S-methylcysteine + H2O + NAD+ 19% of the activity with L-Leu Bacillus licheniformis 3-methylthio-2-oxopropionate + NH3 + NADH
-
?
L-Val + H2O + NAD+ 59% of the activity with L-Leu Bacillus licheniformis 3-methyl-2-oxobutanoate + NH3 + NADH
-
r

Subunits

Subunits Comment Organism
octamer 8 * 44000, SDS-PAGE Bacillus licheniformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
10 mM potassium phosphate buffer, pH 7.4, 0.01% 2-mercaptoethanol, 1 mM EDTA, 1.0 M NaCl, stable for more than 6 months Bacillus licheniformis
65
-
1 h, in presence of 2.5 M NaCl, stable Bacillus licheniformis
70
-
1 h, complete loss of activity Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
reductive amination of 3-methyl-2-oxobutanoate and 2-oxopentanoate Bacillus licheniformis
9.3
-
reductive amination of 4-methyl-2-oxopentanoate Bacillus licheniformis
10.3
-
oxidative deamination of L-Leu in presence of 1.0 M NaCl Bacillus licheniformis

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADPH Bacillus licheniformis
additional information no activity with NADP+ Bacillus licheniformis
NAD+
-
Bacillus licheniformis
NADH
-
Bacillus licheniformis