Cloned (Comment) | Organism |
---|---|
cloned into Escherichia coli JM 109 with a vector plasmid pUC18 | Bacillus licheniformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADH | noncompetitive inhibition with respect to L-Leu | Bacillus licheniformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
4-methyl-2-oxopentanoate | - |
Bacillus licheniformis | |
2.1 | - |
L-Leu | - |
Bacillus licheniformis | |
2.4 | - |
2-oxopentanoate | - |
Bacillus licheniformis | |
3.3 | - |
L-Ile | - |
Bacillus licheniformis | |
3.8 | - |
3-methyl-2-oxobutanoate | - |
Bacillus licheniformis | |
12.5 | - |
L-Val | - |
Bacillus licheniformis | |
330 | - |
NH3 | - |
Bacillus licheniformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
8 * 44000, SDS-PAGE | Bacillus licheniformis |
360000 | - |
gel filtration | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxopentanoate + NH3 + NADH + H+ | - |
Bacillus licheniformis | L-norvaline + H2O + NAD+ | - |
r | |
L-2-aminobutanoate + H2O + NAD+ | 32% of the activity with L-Leu | Bacillus licheniformis | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-Ala + H2O + NAD+ | 10% of the activity with L-Leu | Bacillus licheniformis | 2-oxopropanoate + NH3 + NADH | - |
? | |
L-Ile + H2O + NAD+ | 72% of the activity with L-Leu | Bacillus licheniformis | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-Leu + H2O + NAD+ | - |
Bacillus licheniformis | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-norleucine + H2O + NAD+ | 7% of the activity with L-Leu | Bacillus licheniformis | 2-oxohexanoate + NH3 + NADH | - |
r | |
L-S-methylcysteine + H2O + NAD+ | 19% of the activity with L-Leu | Bacillus licheniformis | 3-methylthio-2-oxopropionate + NH3 + NADH | - |
? | |
L-Val + H2O + NAD+ | 59% of the activity with L-Leu | Bacillus licheniformis | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 44000, SDS-PAGE | Bacillus licheniformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
10 mM potassium phosphate buffer, pH 7.4, 0.01% 2-mercaptoethanol, 1 mM EDTA, 1.0 M NaCl, stable for more than 6 months | Bacillus licheniformis |
65 | - |
1 h, in presence of 2.5 M NaCl, stable | Bacillus licheniformis |
70 | - |
1 h, complete loss of activity | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.6 | - |
reductive amination of 3-methyl-2-oxobutanoate and 2-oxopentanoate | Bacillus licheniformis |
9.3 | - |
reductive amination of 4-methyl-2-oxopentanoate | Bacillus licheniformis |
10.3 | - |
oxidative deamination of L-Leu in presence of 1.0 M NaCl | Bacillus licheniformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADPH | Bacillus licheniformis | |
additional information | no activity with NADP+ | Bacillus licheniformis | |
NAD+ | - |
Bacillus licheniformis | |
NADH | - |
Bacillus licheniformis |