Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3,5-pyrazoledicarboxylate | activates at lower 2-oxoglutarate concentration | Aspergillus niger | |
3,5-pyridinedicarboxylate | activates at lower 2-oxoglutarate concentration | Aspergillus niger | |
DL-2-hydroxyglutarate | activates at lower 2-oxoglutarate concentration | Aspergillus niger | |
Glutarate | activates at lower 2-oxoglutarate concentration | Aspergillus niger | |
Itaconate | activates even in the presence of 10 mM 2-oxoglutarate | Aspergillus niger | |
oxalylglycine | activates at lower 2-oxoglutarate concentration | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-pyridinedicarboxylate | - |
Aspergillus niger | |
2-Methyleneglutarate | - |
Aspergillus niger | |
2-oxoglutarate | shows allosteric properties and a sigmoid response (nH=2.5) towards 2-oxoglutarate saturation | Aspergillus niger | |
isophthalate | - |
Aspergillus niger | |
additional information | not inhibited by D-glutamate, L-glutamine or DL-2-hydroxyglutarate | Aspergillus niger | |
NADP+ | inhibits at higher 2-oxoglutarate levels | Aspergillus niger | |
NH4+ | - |
Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
NADP+ | - |
Aspergillus niger | |
1.05 | - |
NH4+ | - |
Aspergillus niger | |
4.78 | - |
2-oxoglutarate | - |
Aspergillus niger | |
34.7 | - |
L-glutamate | - |
Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Aspergillus niger |
350000 | - |
gel filtration | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus awamori | - |
- |
- |
Aspergillus awamori NCIM 1225 | - |
- |
- |
Aspergillus nidulans | - |
- |
- |
Aspergillus nidulans NCIM 1211 | - |
- |
- |
Aspergillus niger | - |
- |
- |
Aspergillus niger NCIM 565 | - |
- |
- |
Aspergillus oryzae | - |
- |
- |
Aspergillus oryzae NCIM 553 | - |
- |
- |
Aspergillus terreus | - |
- |
- |
Aspergillus terreus NCIM 656 | - |
- |
- |
Saccharomyces cerevisiae | - |
strain Y05499, strain Y10345 and strain Y11672 | - |
Purification (Comment) | Organism |
---|---|
by Cibacron red LS-B dye-affinity purification with a step KCl gradient elution, 17fold purified | Aspergillus nidulans |
by Cibacron red LS-B dye-affinity purification with a step KCl gradient elution, more than 7fold purified to homogeneity | Aspergillus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus nidulans | - |
mycelium | - |
Aspergillus niger | - |
mycelium | - |
Aspergillus oryzae | - |
mycelium | - |
Aspergillus terreus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.37 | - |
crude extract | Aspergillus nidulans |
2.17 | - |
crude extract | Aspergillus niger |
7 | - |
purified enzyme | Aspergillus nidulans |
59.4 | - |
purified enzyme | Aspergillus niger |
Storage Stability | Organism |
---|---|
4°C, 1 week | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NADPH + NH3 | - |
Aspergillus nidulans | L-glutamate + NADP+ + H2O | - |
r | |
2-oxoglutarate + NADPH + NH3 | - |
Aspergillus niger | L-glutamate + NADP+ + H2O | - |
r | |
2-oxoglutarate + NADPH + NH3 | - |
Aspergillus oryzae | L-glutamate + NADP+ + H2O | - |
r | |
2-oxoglutarate + NADPH + NH3 | - |
Aspergillus terreus | L-glutamate + NADP+ + H2O | - |
r | |
2-oxoglutarate + NADPH + NH3 | - |
Aspergillus awamori | L-glutamate + NADP+ + H2O | - |
r | |
2-oxoglutarate + NADPH + NH3 | - |
Aspergillus oryzae NCIM 553 | L-glutamate + NADP+ + H2O | - |
r | |
2-oxoglutarate + NADPH + NH3 | - |
Aspergillus nidulans NCIM 1211 | L-glutamate + NADP+ + H2O | - |
r | |
2-oxoglutarate + NADPH + NH3 | - |
Aspergillus niger NCIM 565 | L-glutamate + NADP+ + H2O | - |
r | |
2-oxoglutarate + NADPH + NH3 | - |
Aspergillus awamori NCIM 1225 | L-glutamate + NADP+ + H2O | - |
r | |
2-oxoglutarate + NADPH + NH3 | - |
Aspergillus terreus NCIM 656 | L-glutamate + NADP+ + H2O | - |
r | |
2-oxoglutarate + NH4+ + NADPH | - |
Aspergillus niger | L-glutamate + NADP+ | - |
? | |
2-oxoglutarate + NH4+ + NADPH | - |
Aspergillus niger NCIM 565 | L-glutamate + NADP+ | - |
? | |
additional information | no reaction with D-glutamate, L-glutamine or DL-2-hydroxyglutarate as possible substrates in place of L-glutamate, methylamine is unable to replace ammonium in the biosynthetic reaction | Aspergillus niger | ? | - |
? | |
additional information | no reaction with D-glutamate, L-glutamine or DL-2-hydroxyglutarate as possible substrates in place of L-glutamate, methylamine is unable to replace ammonium in the biosynthetic reaction | Aspergillus niger NCIM 565 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | x * 50000, SDS-PAGE | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
NADP-GDH | - |
Saccharomyces cerevisiae |
NADP-GDH | - |
Aspergillus nidulans |
NADP-GDH | - |
Aspergillus niger |
NADP-GDH | - |
Aspergillus oryzae |
NADP-GDH | - |
Aspergillus terreus |
NADP-GDH | - |
Aspergillus awamori |
NADP-glutamate dehydrogenase | - |
Saccharomyces cerevisiae |
NADP-glutamate dehydrogenase | - |
Aspergillus nidulans |
NADP-glutamate dehydrogenase | - |
Aspergillus niger |
NADP-glutamate dehydrogenase | - |
Aspergillus oryzae |
NADP-glutamate dehydrogenase | - |
Aspergillus terreus |
NADP-glutamate dehydrogenase | - |
Aspergillus awamori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
reductive amination reaction | Aspergillus niger |
9.75 | - |
oxidative deamination reaction | Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Aspergillus nidulans | |
NADP+ | - |
Aspergillus oryzae | |
NADP+ | - |
Aspergillus terreus | |
NADP+ | - |
Aspergillus awamori | |
NADP+ | stimulates the enzyme at lower 2-oxoglutarate levels | Aspergillus niger | |
NADPH | - |
Aspergillus nidulans | |
NADPH | - |
Aspergillus niger | |
NADPH | - |
Aspergillus oryzae | |
NADPH | - |
Aspergillus terreus | |
NADPH | - |
Aspergillus awamori |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
NH4+ | - |
Aspergillus niger | |
6.9 | - |
isophthalate | - |
Aspergillus niger | |
9.2 | - |
2-Methyleneglutarate | - |
Aspergillus niger | |
202 | - |
2,4-pyridinedicarboxylate | - |
Aspergillus niger |