Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
270000 | - |
- |
Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD(P)+ | Pyrococcus furiosus | - |
2-oxoglutarate + NH3 + NAD(P)H + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD(P)+ | - |
Pyrococcus furiosus | 2-oxoglutarate + NH3 + NAD(P)H + H+ | - |
? | |
L-glutamate + H2O + NAD+ | - |
Pyrococcus furiosus | 2-oxoglutarate + NH3 + NADH + H+ | - |
? | |
L-glutamate + H2O + NADP+ | - |
Pyrococcus furiosus | 2-oxoglutarate + NH3 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
assay at | Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
inactive at temperatures below 40°C, undergoes heat activation above 40°C | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
110 | - |
thermal denaturation starts at 110°C and is completed at 118°C. The process of heat activation from 40 to 80°C is accompanied by a much smaller increase in absorbance at 280 nm and a reversible increase in heat capacity with DELTAcal = 187 Kcal/mol GDH and Tm = 57°C. This absorbance change as well as the moderate increase in heat capacity suggest that thermal activation leads to some exposure of hydrophobic groups to solvent water as the GDH structure is opened slightly. The increase in absorbance at 280 nm during activation is only 12% of that for denaturation | Pyrococcus furiosus |
113 | - |
Tm-value = 113°C | Pyrococcus furiosus |
118 | - |
thermal denaturation starts at 110°C and is comp1eted at 118°C | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | both NAD+ and NADP+ are utilized as cofactors. The inactive enzyme also binds cofactors | Pyrococcus furiosus | |
NADP+ | both NAD+ and NADP+ are utilized as cofactors. The inactive enzyme also binds cofactors | Pyrococcus furiosus |