Cloned (Comment) | Organism |
---|---|
ORF PA3505, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21 (DE3) | Pseudomonas aeruginosa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | establishing of a L-Asp production system consisting of PaeAspDH, Bacillus subtilis malate dehydrogenase and Escherichia coli fumarase, achieving a high level of L-Asp production from fumarate in fed-batch process with a molar conversion yield of 89.4% in LB medium supplemented with fumarate, and 100 mM NH4Cl, overview, or in the same production system with glucose M9 minimal medium containing 50 mM glucose and 80 mM urea as carbon and nitrogen source, respectively | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Pseudomonas aeruginosa | |
0.045 | - |
NADH | pH 8.2, 37°C | Pseudomonas aeruginosa | |
0.052 | - |
NADPH | pH 8.2, 37°C | Pseudomonas aeruginosa | |
0.47 | - |
NAD+ | pH 9.8, 37°C | Pseudomonas aeruginosa | |
0.47 | - |
NADP+ | pH 9.8, 37°C | Pseudomonas aeruginosa | |
2.12 | - |
oxaloacetate | pH 8.2, 37°C, with NADH | Pseudomonas aeruginosa | |
3.14 | - |
oxaloacetate | pH 8.2, 37°C, with NADPH | Pseudomonas aeruginosa | |
4.74 | - |
L-aspartate | pH 9.8, 37°C, with NADP+ | Pseudomonas aeruginosa | |
4.87 | - |
L-aspartate | pH 9.8, 37°C, with NAD+ | Pseudomonas aeruginosa | |
10.1 | - |
NH3 | pH 8.2, 37°C, with NADH | Pseudomonas aeruginosa | |
12.7 | - |
NH3 | pH 8.2, 37°C, with NADPH | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27920 | - |
2 * 27920, sequence calculation, 2 * 28000, SDS-PAGE | Pseudomonas aeruginosa |
28000 | - |
2 * 27920, sequence calculation, 2 * 28000, SDS-PAGE | Pseudomonas aeruginosa |
60000 | - |
gel filtration | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + H2O + NAD(P)+ | Pseudomonas aeruginosa | - |
oxaloacetate + NH3 + NAD(P)H + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
ORF PA3505 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21 (DE3) to homogeneity | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
127 | - |
purified enzyme, substrate L-aspartate, pH 8.2, 37°C | Pseudomonas aeruginosa |
147 | - |
purified enzyme, substrate oxaloacetate, pH 8.2, 37°C | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + H2O + NAD(P)+ | - |
Pseudomonas aeruginosa | oxaloacetate + NH3 + NAD(P)H + H+ | - |
r | |
L-aspartate + H2O + NAD+ | the enzyme is capable of utilizing both NAD/H and NADP/H as coenzymes | Pseudomonas aeruginosa | oxaloacetate + NH3 + NADH + H+ | - |
r | |
L-aspartate + H2O + NADP+ | the enzyme is capable of utilizing both NAD/H and NADP/H as coenzymes | Pseudomonas aeruginosa | oxaloacetate + NH3 + NADPH + H+ | - |
r | |
additional information | the enzyme exhibits a very high specific activity for L-aspartate and oxaloacetate | Pseudomonas aeruginosa | ? | - |
? | |
oxaloacetate + NH3 + NADH + H+ | the enzyme is capable of utilizing both NAD/H and NADP/H as coenzymes | Pseudomonas aeruginosa | L-aspartate + H2O + NAD+ | - |
r | |
oxaloacetate + NH3 + NADPH + H+ | the enzyme is capable of utilizing both NAD/H and NADP/H as coenzymes | Pseudomonas aeruginosa | L-aspartate + H2O + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 27920, sequence calculation, 2 * 28000, SDS-PAGE | Pseudomonas aeruginosa |
More | enzyme homology modeling, overview | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
L-aspDH | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
48 | - |
20 min, Tm of purified enzyme | Pseudomonas aeruginosa |
60 | - |
20 min, Tm of purified enzyme in presence of 0.4 M NaCl or 30% glycerol | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
42.7 | - |
L-aspartate | pH 9.8, 37°C, with NADP+ | Pseudomonas aeruginosa | |
46.1 | - |
NADP+ | pH 9.8, 37°C | Pseudomonas aeruginosa | |
59.7 | - |
L-aspartate | pH 9.8, 37°C, with NAD+ | Pseudomonas aeruginosa | |
62 | - |
NH3 | pH 8.2, 37°C, with NADH | Pseudomonas aeruginosa | |
62.9 | - |
NAD+ | pH 9.8, 37°C | Pseudomonas aeruginosa | |
68.4 | - |
oxaloacetate | pH 8.2, 37°C, with NADH | Pseudomonas aeruginosa | |
70.5 | - |
NADH | pH 8.2, 37°C | Pseudomonas aeruginosa | |
71.6 | - |
oxaloacetate | pH 8.2, 37°C, with NADPH | Pseudomonas aeruginosa | |
77.6 | - |
NH3 | pH 8.2, 37°C, with NADPH | Pseudomonas aeruginosa | |
80.2 | - |
NADPH | pH 8.2, 37°C | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
amination | Pseudomonas aeruginosa |
9.8 | - |
deamination | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is capable of utilizing both NAD/H and NADP/H as coenzymes | Pseudomonas aeruginosa | |
NAD+ | - |
Pseudomonas aeruginosa | |
NADH | - |
Pseudomonas aeruginosa | |
NADP+ | - |
Pseudomonas aeruginosa | |
NADPH | - |
Pseudomonas aeruginosa |
General Information | Comment | Organism |
---|---|---|
evolution | L-aspartate dehydrogenase is a rare member of amino acid dehydrogenase superfamily | Pseudomonas aeruginosa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.11 | - |
NH3 | pH 8.2, 37°C, with NADPH | Pseudomonas aeruginosa | |
6.14 | - |
NH3 | pH 8.2, 37°C, with NADH | Pseudomonas aeruginosa | |
9.01 | - |
L-aspartate | pH 9.8, 37°C, with NADP+ | Pseudomonas aeruginosa | |
12.3 | - |
L-aspartate | pH 9.8, 37°C, with NAD+ | Pseudomonas aeruginosa | |
22.8 | - |
oxaloacetate | pH 8.2, 37°C, with NADPH | Pseudomonas aeruginosa | |
32.3 | - |
oxaloacetate | pH 8.2, 37°C, with NADH | Pseudomonas aeruginosa | |
98.1 | - |
NADP+ | pH 9.8, 37°C | Pseudomonas aeruginosa | |
133.8 | - |
NAD+ | pH 9.8, 37°C | Pseudomonas aeruginosa | |
1542 | - |
NADPH | pH 8.2, 37°C | Pseudomonas aeruginosa | |
1567 | - |
NADH | pH 8.2, 37°C | Pseudomonas aeruginosa |