Cloned (Comment) | Organism |
---|---|
production of the alpha and beta subunits in Escherichia coli | Azospirillum brasilense |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Aminopyridine adenine dinucleotide phosphate | good inhibitor of the holoenzyme, competitive with respect to NADPH | Azospirillum brasilense | |
iodonitrotetrazolium | inhibitor of the L-glutamate:iodonitrotetrazolim oxidoreductase activity of the alpha subunit at concentrations above 0.1 mM | Azospirillum brasilense |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azospirillum brasilense | - |
- |
- |
Purification (Comment) | Organism |
---|---|
of the alpha subunit, using column chromatography on Q-Sepharose and Ultrogel AcA34 | Azospirillum brasilense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADPH + H+ | - |
Azospirillum brasilense | L-glutamate + NADP+ | - |
? | |
additional information | the alpha subunit catalyzes the synthesis of glutamate from L-glutamine and 2-oxoglutarate, provided that a reducing system is present, reducing system: dithionite and methyl viologen | Azospirillum brasilense | ? | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | the alpha subunit contains FMN as flavin cofactor, 0.94 FMN bound per alpha subunit | Azospirillum brasilense | |
flavin | the beta subunit contains the binding site for FAD, 0.83 mol FAD per mol beta subunit | Azospirillum brasilense | |
additional information | the alpha subunit contains the [3Fe-4S] cluster of the enzyme | Azospirillum brasilense | |
NADPH | the beta subunit contains the NADPH binding site of the enzyme | Azospirillum brasilense |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Azospirillum brasilense | |
0.0024 | - |
3-Aminopyridine adenine dinucleotide phosphate | inhibitor of the holoenzyme, competitive with NADPH | Azospirillum brasilense |