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Literature summary for 1.4.1.1 extracted from

  • Yoshida, A.; Freese, E.
    Enzymic properties of Alanine dehydrogenase of Bacillus subtilis (1965), Biochim. Biophys. Acta, 96, 248-262.
    View publication on PubMed

General Stability

General Stability Organism
reversible deactivation by dilution Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 0.1 mM, 57% inhibition Bacillus subtilis
CaCl2 10 mM, 22% inhibition Bacillus subtilis
CoCl2 0.1 mM, 53% inhibition Bacillus subtilis
CuSO4 10 mM, 36% inhibition Bacillus subtilis
HgCl2 0.003 mM, complete inhibition Bacillus subtilis
MnCl2 10 mM, 12% inhibition Bacillus subtilis
MnSO4 10 mM, 50% inhibition Bacillus subtilis
PCMB 0.3 mM, 52% inhibition Bacillus subtilis
Zn2+ 10 mM, 62% inhibition Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
NADH
-
Bacillus subtilis
0.18
-
NAD+
-
Bacillus subtilis
0.53
-
pyruvate
-
Bacillus subtilis
0.54
-
pyruvate
-
Bacillus subtilis
1.73
-
L-Ala
-
Bacillus subtilis
2.5
-
Hydroxypyruvate
-
Bacillus subtilis
7
-
2-Oxohexanoate
-
Bacillus subtilis
12
-
3-methyl-2-oxobutanoate
-
Bacillus subtilis
16
-
glyoxalate
-
Bacillus subtilis
23
-
2-oxobutanoate
-
Bacillus subtilis
33
-
2-oxopentanoate
-
Bacillus subtilis
38
-
NH4+
-
Bacillus subtilis
45
-
4-methyl-2-oxopentanoate
-
Bacillus subtilis
57
-
L-Ser
-
Bacillus subtilis
62
-
L-norvaline
-
Bacillus subtilis
150
-
L-Val
-
Bacillus subtilis
176
-
L-2-aminobutanoate
-
Bacillus subtilis
250
-
L-Ile
-
Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
220000 230000 equilibrium sedimentation Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus subtilis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
enzyme assay Bacillus subtilis

Storage Stability

Storage Stability Organism
4°C, stable for many months Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-ketocaproate + NH3 + NADH
-
Bacillus subtilis 2-aminocaproate + H2O + NAD+
-
?
4-methyl-2-oxopentanoate + NH3 + NADH
-
Bacillus subtilis 2-amino-4-methylpentanoate + NAD+ + H2O
-
?
glyoxalate + NH3 + NADH + H+
-
Bacillus subtilis aminoacetate + H2O + NAD+
-
r
hydroxypyruvate + NH3 + NADH
-
Bacillus subtilis L-Ser + NAD+ + H2O
-
?
L-2-aminobutanoate + H2O + NAD+ 13.3% of the activity with L-Ala Bacillus subtilis 2-oxobutanoate + NH3 + NADH + H+
-
r
L-Ala + H2O + NAD+
-
Bacillus subtilis pyruvate + NH3 + NADH
-
r
L-Ile + H2O + NAD+ 5% of the activity with L-Ala Bacillus subtilis 2-oxo-3-methylpentanoate + NH3 + NADH
-
?
L-norvaline + H2O + NAD+
-
Bacillus subtilis 2-oxopentanoate + NH3 + NADH
-
r
L-Ser + H2O + NAD+
-
Bacillus subtilis 3-hydroxypyruvate + NH3 + NADH
-
?
L-Val + H2O + NAD+ 9% of the activity with L-Ala Bacillus subtilis 3-methyl-2-oxobutanoate + NH3 + NADH
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information amination: maximal turnover is about 650000 per min, deamination: maximal turnover is 54000 per min Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8 9 amination Bacillus subtilis
10 10.5 oxidative deamination Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
7.2 10 pH 7.2: about 10% of activity maximum, pH 10.0: about 30% of activity maximum, reductive amination Bacillus subtilis
8 11 pH 8: about 35% of activity maximum, pH 11: about 75% of activity maximum, oxidative deamination Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
rapid and complete inactivation below Bacillus subtilis
5
-
stable above Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bacillus subtilis
NADH
-
Bacillus subtilis