Application | Comment | Organism |
---|---|---|
additional information | species-specific preferential inhibitor binding, different binding mode for the same inhibitor in the two catalytically identical enzymes human DHODH and Plasmodium DHODH | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
into vector pRSET and expressed in either freshly transformed or glycerol stocked Escherichia coli BL21-CodonPlus (DE3)-RIL | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
by sitting-drop vapor-diffusion technique, to 2.4 A resolution | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | brequinar and DPC-AE661100 have no effect, even at a concentration of 1 mM | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0131 | - |
2,3-dimethoxy-5-methyl-6-(3-methyl-2-butenyl)-1,4-benzoquinone | at saturating concentration of dihydroorotate | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
gel filtration | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | Q08210 | synthetic construct | - |
Purification (Comment) | Organism |
---|---|
soniciation and gel filtration | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroorotate + 2,3-dimethoxy-5-methyl-6-(3-methyl-2-butenyl)-1,4-benzoquinone | - |
Plasmodium falciparum | orotate + reduced 2,3-dimethoxy-5-methyl-6-(3-methyl-2-butenyl)-1,4-benzoquinone | - |
? | |
dihydroorotate + 2,6-dichloroindophenol | - |
Plasmodium falciparum | orotate + reduced 2,6-dichloroindophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHODH | - |
Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
2,3-dimethoxy-5-methyl-6-(3-methyl-2-butenyl)-1,4-benzoquinone | at saturating concentration of dihydroorotate | Plasmodium falciparum |