Cloned (Comment) | Organism |
---|---|
expression of T168A mutant enzyme in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
T168A | thermostability is markedly decreased, 80% lowered activity in ferricinum assay | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
T168A mutation found in patients with medium-chain acyl-CoA deficiency | - |
Purification (Comment) | Organism |
---|---|
recombinant T168A mutant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
octanoyl-CoA + recombinant beta-Y16L electron transferring flavoprotein | - |
Homo sapiens | trans-oct-2-enoyl-CoA + reduced recombinant beta-Y16L electron transferring flavoprotein | - |
r | |
octanoyl-CoA + recombinant electron transferring flavoprotein | - |
Homo sapiens | trans-oct-2-enoyl-CoA + reduced recombinant electron transferring flavoprotein | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
41 | - |
wild-type enzyme: almost no loss of activity after 1 h at 41°C, T168A mutant enzyme: complete inactivation after 20 min at 41°C | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
recombinant electron transferring flavoprotein | T168A mutant enzyme | Homo sapiens | |
1.67 | - |
recombinant beta-Y16L electron transferring flavoprotein | T168A mutant enzyme | Homo sapiens | |
14.7 | - |
recombinant electron transferring flavoprotein | wild-type enzyme | Homo sapiens | |
18.3 | - |
recombinant beta-Y16L electron transferring flavoprotein | wild-type enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 10 | T168A mutant | Homo sapiens |
10 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | T168A mutant enzyme, approx. 20% of maximum activity at pH 6.0, sigmoidal increase between pH 5.5 and pH 9.0, approx. 80% of maximal activity at pH 7.8 | Homo sapiens |
7 | 10 | approx. 20% of maximum activity at pH 7.5, sigmoidal increase between pH 7.0 and pH 10.0, approx. 80% of maximal activity at pH 9.0 | Homo sapiens |