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Literature summary for 1.3.8.1 extracted from

  • Shirao, K.; Okada, S.; Tajima, G.; Tsumura, M.; Hara, K.; Yasunaga, S.; Ohtsubo, M.; Hata, I.; Sakura, N.; Shigematsu, Y.; Takihara, Y.; Kobayashi, M.
    Molecular pathogenesis of a novel mutation, G108D, in short-chain acyl-CoA dehydrogenase identified in subjects with short-chain acyl-CoA dehydrogenase deficiency (2010), Hum. Genet., 127, 619-628.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
SCAD DNA and amino acid sequence determination and analysis of wild-type and mutant enzymes, coexpression iin HEK-293 cells, expression of fluorescent-labeled enzyme mutants in U2-OS cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
E344G site-directed mutagenesis, the SCAD mutant shows reduced activity compared to the wild-typ enzyme, but does not influence the wild-type SCAD activity when co-transfected in HEK-293 cells Homo sapiens
G108D site-directed mutagenesis, the SCAD mutant shows reduced activity compared to the wild-typ enzyme, but does not influence the wild-type SCAD activity when co-transfected in HEK-293 cells Homo sapiens
P55L site-directed mutagenesis, the SCAD mutant shows reduced activity compared to the wild-typ enzyme, but does not influence the wild-type SCAD activity when co-transfected in HEK-293 cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
butanoyl-CoA + acceptor Homo sapiens
-
but-2-enoyl-CoA + reduced acceptor
-
?
hexanoyl-CoA + acceptor Homo sapiens
-
hex-2-enoyl-CoA + reduced acceptor
-
?
pentanoyl-CoA + acceptor Homo sapiens
-
pent-2-enoyl-CoA + reduced acceptor
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens D4QEZ8
-
-

Source Tissue

Source Tissue Comment Organism Textmining
fibroblast
-
Homo sapiens
-
HEK-293 cell
-
Homo sapiens
-
lymphocyte
-
Homo sapiens
-
additional information SCAD activity levels in different tissues vary greatly, immunohistochemic analysis, overview Homo sapiens
-
muscle
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
butanoyl-CoA + acceptor
-
Homo sapiens but-2-enoyl-CoA + reduced acceptor
-
?
butanoyl-CoA + FAD
-
Homo sapiens but-2-enoyl-CoA + FADH2
-
?
hexanoyl-CoA + acceptor
-
Homo sapiens hex-2-enoyl-CoA + reduced acceptor
-
?
hexanoyl-CoA + FAD
-
Homo sapiens hex-2-enoyl-CoA + FADH2
-
?
pentanoyl-CoA + acceptor
-
Homo sapiens pent-2-enoyl-CoA + reduced acceptor
-
?
pentanoyl-CoA + FAD
-
Homo sapiens pent-2-enoyl-CoA + FADH2
-
?

Synonyms

Synonyms Comment Organism
butyryl-CoA dehydrogenase
-
Homo sapiens
SCAD
-
Homo sapiens
short-chain acyl-CoA dehydrogenase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
26 41 activities of SCAD constructs are entirely lower at 26°C incubation temperature and slightly higher at 41°C incubation temperature than those at 37°C incubation temperature Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD
-
Homo sapiens

General Information

General Information Comment Organism
metabolism mutations in the gene encodine acyl-CoA dehydrogenase, ACAD, cause alterations in SCAD activity, overview Homo sapiens
physiological function SCAD is a mitochondrial enzyme involved in the beta-oxidation of fatty acids and mediates the metabolic transition from acyl-CoA with four or six carbon chains to 2-enoyl-CoA in the first step of the beta-oxidation spiral. Genetic defect of SCAD cause clinical symptoms such as progressive psychomotor retardation, muscle hypotonia, and myopathy Homo sapiens