Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.8.1 extracted from

  • McMahon, B.; Gallagher, M.E.; Mayhew, S.G.
    The protein coded by the PP2216 gene of Pseudomonas putida KT2440 is an acyl-CoA dehydrogenase that oxidises only short-chain aliphatic substrates (2005), FEMS Microbiol. Lett., 250, 121-127.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
into plasmid pRSET B and overexpressed in Escherichia coli C41(DE3) Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02547
-
heptanoyl-CoA
-
Pseudomonas putida
0.04231
-
octanoyl-CoA
-
Pseudomonas putida
0.09303
-
hexanoyl-CoA
-
Pseudomonas putida
0.1238
-
valeryl-CoA
-
Pseudomonas putida
0.1253
-
butyryl-CoA
-
Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38500
-
4 * 38500, SDS-PAGE, 4 * 40529, amino acid sequence analysis, 4 * 40488, MALDI TOF mass spectroscopy Pseudomonas putida
40488
-
4 * 38500, SDS-PAGE, 4 * 40529, amino acid sequence analysis, 4 * 40488, MALDI TOF mass spectroscopy Pseudomonas putida
40529
-
4 * 38500, SDS-PAGE, 4 * 40529, amino acid sequence analysis, 4 * 40488, MALDI TOF mass spectroscopy Pseudomonas putida
160000
-
gel filtration Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida KT 2240
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by successive fractionations of anion-exchange chromatography, ammonium sulfate precipitation and gel filtration, to more than 99% homogeneity Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.13
-
with hexanoyl-CoA as substrate Pseudomonas putida
0.47
-
with 0.21 mM butyryl-CoA as substrate at pH 8.0 Pseudomonas putida
0.86
-
with valeryl-CoA as substrate Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
butyryl-CoA + electron acceptor
-
Pseudomonas putida 2-butenoyl-CoA + reduced acceptor
-
?
butyryl-CoA + electron acceptor
-
Pseudomonas putida KT 2240 2-butenoyl-CoA + reduced acceptor
-
?
heptanoyl-CoA + electron acceptor low activity Pseudomonas putida 2-heptenoyl-CoA + reduced acceptor
-
?
heptanoyl-CoA + electron acceptor low activity Pseudomonas putida KT 2240 2-heptenoyl-CoA + reduced acceptor
-
?
hexanoyl-CoA + electron acceptor
-
Pseudomonas putida 2-hexenoyl-CoA + reduced acceptor
-
?
hexanoyl-CoA + electron acceptor
-
Pseudomonas putida KT 2240 2-hexenoyl-CoA + reduced acceptor
-
?
additional information fails to oxidize propionyl-CoA, inactive with the CoA derivatives of all phenylalkanoates, the enzyme is not involved in the beta-oxidation of aromatic compounds Pseudomonas putida ?
-
?
additional information fails to oxidize propionyl-CoA, inactive with the CoA derivatives of all phenylalkanoates, the enzyme is not involved in the beta-oxidation of aromatic compounds Pseudomonas putida KT 2240 ?
-
?
octanoyl-CoA + electron acceptor low activity Pseudomonas putida 2-octenoyl-CoA + reduced acceptor
-
?
octanoyl-CoA + electron acceptor low activity Pseudomonas putida KT 2240 2-octenoyl-CoA + reduced acceptor
-
?
valeryl-CoA + electron acceptor
-
Pseudomonas putida 2-pentenoyl-CoA + reduced acceptor
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 38500, SDS-PAGE, 4 * 40529, amino acid sequence analysis, 4 * 40488, MALDI TOF mass spectroscopy Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00183
-
octanoyl-CoA
-
Pseudomonas putida
0.00567
-
heptanoyl-CoA
-
Pseudomonas putida
0.293
-
butyryl-CoA at pH 5.5 Pseudomonas putida
0.2992
-
hexanoyl-CoA
-
Pseudomonas putida
0.5883
-
butyryl-CoA at pH 6.0 Pseudomonas putida
0.7267
-
butyryl-CoA at pH 8.0 Pseudomonas putida
0.73
-
butyryl-CoA at pH 6.5 Pseudomonas putida
0.967
-
butyryl-CoA at pH 7.0 Pseudomonas putida
0.967
-
butyryl-CoA at pH 7.5 Pseudomonas putida
1.743
-
butyryl-CoA
-
Pseudomonas putida
2.403
-
valeryl-CoA
-
Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
Pseudomonas putida

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8
-
Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
FAD one per subunit Pseudomonas putida