Cloned (Comment) | Organism |
---|---|
into plasmid pRSET B and overexpressed in Escherichia coli C41(DE3) | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02547 | - |
heptanoyl-CoA | - |
Pseudomonas putida | |
0.04231 | - |
octanoyl-CoA | - |
Pseudomonas putida | |
0.09303 | - |
hexanoyl-CoA | - |
Pseudomonas putida | |
0.1238 | - |
valeryl-CoA | - |
Pseudomonas putida | |
0.1253 | - |
butyryl-CoA | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38500 | - |
4 * 38500, SDS-PAGE, 4 * 40529, amino acid sequence analysis, 4 * 40488, MALDI TOF mass spectroscopy | Pseudomonas putida |
40488 | - |
4 * 38500, SDS-PAGE, 4 * 40529, amino acid sequence analysis, 4 * 40488, MALDI TOF mass spectroscopy | Pseudomonas putida |
40529 | - |
4 * 38500, SDS-PAGE, 4 * 40529, amino acid sequence analysis, 4 * 40488, MALDI TOF mass spectroscopy | Pseudomonas putida |
160000 | - |
gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida KT 2240 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by successive fractionations of anion-exchange chromatography, ammonium sulfate precipitation and gel filtration, to more than 99% homogeneity | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.13 | - |
with hexanoyl-CoA as substrate | Pseudomonas putida |
0.47 | - |
with 0.21 mM butyryl-CoA as substrate at pH 8.0 | Pseudomonas putida |
0.86 | - |
with valeryl-CoA as substrate | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butyryl-CoA + electron acceptor | - |
Pseudomonas putida | 2-butenoyl-CoA + reduced acceptor | - |
? | |
butyryl-CoA + electron acceptor | - |
Pseudomonas putida KT 2240 | 2-butenoyl-CoA + reduced acceptor | - |
? | |
heptanoyl-CoA + electron acceptor | low activity | Pseudomonas putida | 2-heptenoyl-CoA + reduced acceptor | - |
? | |
heptanoyl-CoA + electron acceptor | low activity | Pseudomonas putida KT 2240 | 2-heptenoyl-CoA + reduced acceptor | - |
? | |
hexanoyl-CoA + electron acceptor | - |
Pseudomonas putida | 2-hexenoyl-CoA + reduced acceptor | - |
? | |
hexanoyl-CoA + electron acceptor | - |
Pseudomonas putida KT 2240 | 2-hexenoyl-CoA + reduced acceptor | - |
? | |
additional information | fails to oxidize propionyl-CoA, inactive with the CoA derivatives of all phenylalkanoates, the enzyme is not involved in the beta-oxidation of aromatic compounds | Pseudomonas putida | ? | - |
? | |
additional information | fails to oxidize propionyl-CoA, inactive with the CoA derivatives of all phenylalkanoates, the enzyme is not involved in the beta-oxidation of aromatic compounds | Pseudomonas putida KT 2240 | ? | - |
? | |
octanoyl-CoA + electron acceptor | low activity | Pseudomonas putida | 2-octenoyl-CoA + reduced acceptor | - |
? | |
octanoyl-CoA + electron acceptor | low activity | Pseudomonas putida KT 2240 | 2-octenoyl-CoA + reduced acceptor | - |
? | |
valeryl-CoA + electron acceptor | - |
Pseudomonas putida | 2-pentenoyl-CoA + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 38500, SDS-PAGE, 4 * 40529, amino acid sequence analysis, 4 * 40488, MALDI TOF mass spectroscopy | Pseudomonas putida |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00183 | - |
octanoyl-CoA | - |
Pseudomonas putida | |
0.00567 | - |
heptanoyl-CoA | - |
Pseudomonas putida | |
0.293 | - |
butyryl-CoA | at pH 5.5 | Pseudomonas putida | |
0.2992 | - |
hexanoyl-CoA | - |
Pseudomonas putida | |
0.5883 | - |
butyryl-CoA | at pH 6.0 | Pseudomonas putida | |
0.7267 | - |
butyryl-CoA | at pH 8.0 | Pseudomonas putida | |
0.73 | - |
butyryl-CoA | at pH 6.5 | Pseudomonas putida | |
0.967 | - |
butyryl-CoA | at pH 7.0 | Pseudomonas putida | |
0.967 | - |
butyryl-CoA | at pH 7.5 | Pseudomonas putida | |
1.743 | - |
butyryl-CoA | - |
Pseudomonas putida | |
2.403 | - |
valeryl-CoA | - |
Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | one per subunit | Pseudomonas putida |