Cloned (Comment) | Organism |
---|---|
expression of wild-type, E368G/G247E, E368D, E368Q and E368G mutant enzyme in Escherichia coli | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
E368D | mutant exhibits enzyme activity | Rattus norvegicus |
E368G | inactive enzyme, E368 is responsible for catalytic activity | Rattus norvegicus |
E368G/G247E | mutant exhibits enzyme activity | Rattus norvegicus |
E368L | inactive enzyme, E368 is responsible for catalytic activity | Rattus norvegicus |
E368Q | inactive enzyme, E368 is responsible for catalytic activity | Rattus norvegicus |
E368R | inactive enzyme, E368 is responsible for catalytic activity | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0006 | - |
butyryl-CoA | wild-type enzyme | Rattus norvegicus | |
0.0011 | - |
octanoyl-CoA | E368G/G247E double mutant enzyme | Rattus norvegicus | |
0.0015 | - |
butyryl-CoA | E368D mutant enzyme | Rattus norvegicus | |
0.0017 | - |
hexanoyl-CoA | E368G/G247E double mutant enzyme | Rattus norvegicus | |
0.002 | - |
butyryl-CoA | E368G/G247E double mutant enzyme | Rattus norvegicus | |
0.0038 | - |
hexanoyl-CoA | wild-type enzyme | Rattus norvegicus | |
0.005 | - |
octanoyl-CoA | E368D mutant enzyme | Rattus norvegicus | |
0.005 | - |
hexanoyl-CoA | E368D mutant enzyme | Rattus norvegicus | |
0.0081 | - |
octanoyl-CoA | wild-type enzyme | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type enzyme purified, E368Q, E368G and E368L mutant enzyme, partially purified | Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.3 | - |
E368G/G247E double mutant enzyme, electron transfer protein as electron acceptor | Rattus norvegicus |
1.9 | - |
E368D mutant enzyme, electron transfer protein as electron acceptor | Rattus norvegicus |
3.8 | - |
wild-type enzyme, electron transfer protein as electron acceptor | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
octanoyl-CoA + electron transfer protein | - |
Rattus norvegicus | 2-octenoyl-CoA + reduced acceptor | - |
? |