Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-butenoyl-CoA + reduced electron transfer flavoprotein | Megasphaera elsdenii | enzyme functions as C3-C6 enoyl-CoA reductase in vivo and catalyzes the oxidation of butyryl-CoA and related substrates in vitro | butanoyl-CoA + electron transfer protein | - |
r | |
2-hexenoyl-CoA + reduced electron transfer flavoprotein | Megasphaera elsdenii | enzyme functions as C3-C6 enoyl-CoA reductase in vivo and catalyzes the oxidation of butyryl-CoA and related substrates in vitro | hexanoyl-CoA + electron transfer protein | - |
r | |
2-pentenoyl-CoA + reduced electron transfer flavoprotein | Megasphaera elsdenii | enzyme functions as C3-C6 enoyl-CoA reductase in vivo and catalyzes the oxidation of butyryl-CoA and related substrates in vitro | pentanoyl-CoA + electron transfer protein | - |
r | |
2-propenoyl-CoA + reduced electron transfer flavoprotein | Megasphaera elsdenii | enzyme functions as C3-C6 enoyl-CoA reductase in vivo and catalyzes the oxidation of butyryl-CoA and related substrates in vitro | propanoyl-CoA + electron transfer protein | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Megasphaera elsdenii | - |
- |
- |
Storage Stability | Organism |
---|---|
short term: 4°C, 0.1 M potassium phosphate buffer, pH 7, 0.02% sodium azide or 0.002% chlorhexidine, long term: frozen in ammonium sulfate | Megasphaera elsdenii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-butenoyl-CoA + reduced electron transfer flavoprotein | enzyme functions as C3-C6 enoyl-CoA reductase in vivo and catalyzes the oxidation of butyryl-CoA and related substrates in vitro | Megasphaera elsdenii | butanoyl-CoA + electron transfer protein | - |
r | |
2-hexenoyl-CoA + reduced electron transfer flavoprotein | enzyme functions as C3-C6 enoyl-CoA reductase in vivo and catalyzes the oxidation of butyryl-CoA and related substrates in vitro | Megasphaera elsdenii | hexanoyl-CoA + electron transfer protein | - |
r | |
2-pentenoyl-CoA + reduced electron transfer flavoprotein | enzyme functions as C3-C6 enoyl-CoA reductase in vivo and catalyzes the oxidation of butyryl-CoA and related substrates in vitro | Megasphaera elsdenii | pentanoyl-CoA + electron transfer protein | - |
r | |
2-propenoyl-CoA + reduced electron transfer flavoprotein | enzyme functions as C3-C6 enoyl-CoA reductase in vivo and catalyzes the oxidation of butyryl-CoA and related substrates in vitro | Megasphaera elsdenii | propanoyl-CoA + electron transfer protein | - |
r | |
3-hydroxybutyryl-CoA + acceptor | - |
Megasphaera elsdenii | acetoacetyl-CoA + reduced acceptor | - |
? | |
butyryl-CoA + electron acceptor | terminal electron acceptor 2,6-dichlorophenol-indophenol | Megasphaera elsdenii | 2-butenoyl-CoA + reduced acceptor | - |
? | |
butyryl-CoA + electron acceptor | terminal electron acceptor iodonitrotetrazolium chloride | Megasphaera elsdenii | 2-butenoyl-CoA + reduced acceptor | - |
? | |
butyryl-CoA + electron acceptor | intermediate electron acceptor phenazine methosulfate | Megasphaera elsdenii | 2-butenoyl-CoA + reduced acceptor | - |
? | |
cyclobutanecarboxyl-CoA + phenazine ethosulfate + dichloroindophenol | - |
Megasphaera elsdenii | cyclo-2-butenecarboxyl-CoA + reduced acceptor | - |
? | |
cyclopentanecarboxyl-CoA + phenazine ethosulfate + dichloroindophenol | - |
Megasphaera elsdenii | cyclo-2-pentenecarboxyl-CoA + reduced acceptor | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | tightly bound | Megasphaera elsdenii | |
FAD | 1 mol per mol of subunit | Megasphaera elsdenii |