KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
butyryl-CoA | - |
Megasphaera elsdenii | |
0.016 | - |
pentanoyl-CoA | - |
Megasphaera elsdenii | |
0.053 | - |
hexanoyl-CoA | - |
Megasphaera elsdenii | |
0.082 | - |
cyclohexanecarboxyl-CoA | - |
Megasphaera elsdenii | |
0.095 | - |
cyclopentanecarboxyl-CoA | - |
Megasphaera elsdenii | |
0.097 | - |
4-methylpentanoyl-CoA | - |
Megasphaera elsdenii | |
0.119 | - |
cyclobutanecarboxyl-CoA | - |
Megasphaera elsdenii | |
0.13 | 0.15 | propionyl-CoA | - |
Megasphaera elsdenii | |
0.161 | - |
2-methylpropionyl-CoA | - |
Megasphaera elsdenii | |
0.198 | - |
cycloheptanecarboxyl-CoA | - |
Megasphaera elsdenii | |
0.213 | - |
S-2-methylbutyryl-CoA | - |
Megasphaera elsdenii | |
0.59 | - |
phenazine ethosulfate | phenazine ethosulfate as intermediate electron carrier | Megasphaera elsdenii | |
0.8 | - |
phenazine methosulfate | - |
Megasphaera elsdenii | |
0.83 | - |
phenazine methosulfate | phenazine methosulfate as intermediate electron carrier | Megasphaera elsdenii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Megasphaera elsdenii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-methylbutyryl-CoA + phenazine methosulfate + dichloroindophenol | 6.0% of activity with butyryl-CoA | Megasphaera elsdenii | (S)-2-methyl-2-butenoyl-CoA + reduced acceptor | - |
? | |
2-methylpropionyl-CoA + phenazine methosulfate + dichloroindophenol | 1.5% of activity with butyryl-CoA | Megasphaera elsdenii | 2-methylpropenoyl-CoA + reduced acceptor | - |
? | |
2-propenoyl-CoA + reduced electron transfer flavoprotein | - |
Megasphaera elsdenii | propanoyl-CoA + electron transfer protein | - |
? | |
4-methylpentanoyl-CoA + phenazine methosulfate + dichloroindophenol | 18.0% of activity with butyryl-CoA | Megasphaera elsdenii | 4-methyl-2-pentenoyl-CoA + reduced acceptor | - |
? | |
butyryl-CoA + electron acceptor | intermediate electron acceptors phenazine methosulfate or phenazine ethosulfate | Megasphaera elsdenii | 2-butenoyl-CoA + reduced acceptor | - |
? | |
butyryl-CoA + electron acceptor | intermediate electron acceptors phenazine methosulfate or Meldola' s Blue (i.e. 8-dimethylamino-2,3-benzophenoxazinium chloride) | Megasphaera elsdenii | 2-butenoyl-CoA + reduced acceptor | - |
? | |
butyryl-CoA + electron acceptor | terminal electron acceptor 2,6-dichlorophenol-indophenol | Megasphaera elsdenii | 2-butenoyl-CoA + reduced acceptor | - |
? | |
butyryl-CoA + electron acceptor | terminal electron acceptor iodonitrotetrazolium chloride | Megasphaera elsdenii | 2-butenoyl-CoA + reduced acceptor | - |
? | |
butyryl-CoA + electron acceptor | intermediate electron acceptor phenazine methosulfate | Megasphaera elsdenii | 2-butenoyl-CoA + reduced acceptor | - |
? | |
cyclobutanecarboxyl-CoA + phenazine ethosulfate + dichloroindophenol | 1.0% of activity with butyryl-CoA | Megasphaera elsdenii | cyclo-2-butenecarboxyl-CoA + reduced acceptor | - |
? | |
cycloheptanecarboxyl-CoA + phenazine ethosulfate + dichloroindophenol | 3.2% of activity with butyryl-CoA | Megasphaera elsdenii | cyclo-2-heptenecarboxyl-CoA + reduced acceptor | - |
? | |
cyclohexanecarboxyl-CoA + phenazine ethosulfate + dichloroindophenol | 6.7% of activity with butyryl-CoA | Megasphaera elsdenii | cyclo-2-hexenecarboxyl-CoA + reduced acceptor | - |
? | |
cyclopentanecarboxyl-CoA + phenazine ethosulfate + dichloroindophenol | 2.1% of activity with butyryl-CoA | Megasphaera elsdenii | cyclo-2-pentenecarboxyl-CoA + reduced acceptor | - |
? | |
hexanoyl-CoA + phenazine methosulfate + dichloroindophenol | 4.2% of activity with butyryl-coA | Megasphaera elsdenii | 2-hexenoyl-CoA + reduced acceptor | - |
? | |
hexanoyl-CoA + phenazine methosulfate + dichloroindophenol | acceptor Meldola's Blue and iodonitrotetrazolium chloride | Megasphaera elsdenii | 2-hexenoyl-CoA + reduced acceptor | - |
? | |
pentanoyl-CoA + phenazine methosulfate | 45% of activity with butyryl-CoA, 2,6-dichlorophenolindophenol as final electron acceptor | Megasphaera elsdenii | 2-pentenoyl-CoA + reduced acceptor | - |
? | |
pentanoyl-CoA + phenazine methosulfate | pentanoyl-CoA i.e. valeryl-CoA | Megasphaera elsdenii | 2-pentenoyl-CoA + reduced acceptor | - |
? | |
S-butyrylpantetheine + phenazine ethosulfate + dichloroindophenol | 51.0% of activity with butyryl-CoA | Megasphaera elsdenii | S-but-2-eneoylpantetheine + reduced acceptor | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.43 | - |
butyryl-CoA | phenazine ethosulfate as intermediate electron carrier | Megasphaera elsdenii | |
20.7 | - |
butyryl-CoA | - |
Megasphaera elsdenii | |
20.8 | - |
butyryl-CoA | phenazine methosulfate as intermediate electron carrier | Megasphaera elsdenii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Megasphaera elsdenii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9 | 57% activity at pH 7.6, 72% activity at pH 9 | Megasphaera elsdenii |