Cloned (Comment) | Organism |
---|---|
genes Ferp_1184-Ferp_1187, recombinant expression of codon-optimized genes encoding the enzyme from Ferroglobus placidus in the thermophilic lignocellulolytic host Caldicellulosiruptor bescii strain JWCB018, coexpression with benzoyl-CoA ligase and a putative benzoate transporter (encoded by gene Ferp_1370), quantitative RT-PCR enzyme expression analysis, method evaluation. Peptide evidence is not detected for one of the four subunits of benzoyl-CoA reductase (Ferp_1185), or for the putative benzoate transporter (Ferp_1370) | Ferroglobus placidus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | coexpression of benzoyl-CoA reductase with benzoyl-CoA ligase and a putative benzoate transporter in Caldicellulosiruptor bescii strain JWCB018, variable effectiveness of the ribosome binding site sequences utilized in this synthetic operon is observed. The engineered strains do not degrade benzoate. Although the heterologously expressed gene encoding benzoyl-CoA ligase yields a protein that is catalytically active in vitro, expression in Caldicellulosiruptor bescii of six benzoate catabolism-related genes combined in a synthetic operon yields mixed results | Ferroglobus placidus |
Organism | UniProt | Comment | Textmining |
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Ferroglobus placidus | - |
- |
- |
Ferroglobus placidus AEDII12DO | - |
- |
- |
Ferroglobus placidus DSM 10642 | - |
- |
- |