BRENDA - Enzyme Database
show all sequences of 1.3.7.13

Two unrelated 8-vinyl reductases ensure production of mature chlorophylls in Acaryochloris marina

Chen, G.E.; Hitchcock, A.; Jackson, P.J.; Chaudhuri, R.R.; Dickman, M.J.; Hunter, C.N.; Canniffe, D.P.; J. Bacteriol. 198, 1393-1400 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene bciB or frhB, DNA and amino acid sequence determination and analysis, phylogenetic analysis, frhB is introduced into an 8-vinyl chlorophyll a-producing DELTAbciB strain of Synechocystis sp. strain PCC 6803, and restores synthesis of the pigment with an ethyl group at C-8, demonstrating its activity as 8-vinyl reductase, subcloning in Escherichia coli strain JM109
Acaryochloris marina
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+ + NADPH + H+
Acaryochloris marina
-
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
-
?
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+ + NADPH + H+
Acaryochloris marina MBIC11017
-
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acaryochloris marina
B0CAB4
-
-
Acaryochloris marina MBIC11017
B0CAB4
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+ + NADPH + H+
-
745221
Acaryochloris marina
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
-
-
?
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+ + NADPH + H+
-
745221
Acaryochloris marina MBIC11017
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 45492, sequence calculation
Acaryochloris marina
Cofactor
Cofactor
Commentary
Organism
Structure
ferredoxin [iron-sulfur] cluster
-
Acaryochloris marina
NADPH
-
Acaryochloris marina
Cloned(Commentary) (protein specific)
Commentary
Organism
gene bciB or frhB, DNA and amino acid sequence determination and analysis, phylogenetic analysis, frhB is introduced into an 8-vinyl chlorophyll a-producing DELTAbciB strain of Synechocystis sp. strain PCC 6803, and restores synthesis of the pigment with an ethyl group at C-8, demonstrating its activity as 8-vinyl reductase, subcloning in Escherichia coli strain JM109
Acaryochloris marina
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ferredoxin [iron-sulfur] cluster
-
Acaryochloris marina
NADPH
-
Acaryochloris marina
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+ + NADPH + H+
Acaryochloris marina
-
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
-
?
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+ + NADPH + H+
Acaryochloris marina MBIC11017
-
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+ + NADPH + H+
-
745221
Acaryochloris marina
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
-
-
?
3,8-divinyl protochlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + H+ + NADPH + H+
-
745221
Acaryochloris marina MBIC11017
protochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 45492, sequence calculation
Acaryochloris marina
General Information
General Information
Commentary
Organism
evolution
two unrelated classes of 8-vinyl reductases are known to exist in oxygenic phototrophs, BciA and BciB. Transcript and proteomic analysis of Acaryochloris marina reveal that both bciA and bciB are expressed and their encoded proteins are present in the cell, possibly in order to ensure that all synthesized chlorophyll pigment carries an ethyl group at C-8. The presence of two 8-vinyl reductases is unique for cyanobacteria. The phylogenetic positions of Acaryochloris marina BciA and BciB are both broadly consistent with those shown for Acaryochloris marina in the 16S rRNA trees, suggesting that the bciA and bciB genes have not been acquired by horizontal transfer. However, the positions of Synechococcus spp. in the BciA tree and the clade containing the green sulfur bacteria in the BciB tree are inconsistent with the 16S rRNA phylogeny, indicating that there may have been lateral transfer events during the evolution of both bciA and bciB
Acaryochloris marina
malfunction
loss of 8-vinyl reductase activity in Acaryochloris marina results in the production of 8-vinyl-Chl a and 8-vinyl-Chl d with a negative effect on viability of the cells
Acaryochloris marina
additional information
the enzyme encoded by gene frhB shows 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (ferredoxin) activity and shall be renamed bciB
Acaryochloris marina
physiological function
functional complementation of an enzyme-deficient DELTAbciB Synechocystis sp. strain PCC 6803
Acaryochloris marina
General Information (protein specific)
General Information
Commentary
Organism
evolution
two unrelated classes of 8-vinyl reductases are known to exist in oxygenic phototrophs, BciA and BciB. Transcript and proteomic analysis of Acaryochloris marina reveal that both bciA and bciB are expressed and their encoded proteins are present in the cell, possibly in order to ensure that all synthesized chlorophyll pigment carries an ethyl group at C-8. The presence of two 8-vinyl reductases is unique for cyanobacteria. The phylogenetic positions of Acaryochloris marina BciA and BciB are both broadly consistent with those shown for Acaryochloris marina in the 16S rRNA trees, suggesting that the bciA and bciB genes have not been acquired by horizontal transfer. However, the positions of Synechococcus spp. in the BciA tree and the clade containing the green sulfur bacteria in the BciB tree are inconsistent with the 16S rRNA phylogeny, indicating that there may have been lateral transfer events during the evolution of both bciA and bciB
Acaryochloris marina
malfunction
loss of 8-vinyl reductase activity in Acaryochloris marina results in the production of 8-vinyl-Chl a and 8-vinyl-Chl d with a negative effect on viability of the cells
Acaryochloris marina
additional information
the enzyme encoded by gene frhB shows 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (ferredoxin) activity and shall be renamed bciB
Acaryochloris marina
physiological function
functional complementation of an enzyme-deficient DELTAbciB Synechocystis sp. strain PCC 6803
Acaryochloris marina
Other publictions for EC 1.3.7.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738542
Chen
Two unrelated 8-vinyl reductas ...
Acaryochloris marina
J. Bacteriol.
198
1393-1400
2016
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745221
Chen
Two unrelated 8-vinyl reducta ...
Acaryochloris marina, Acaryochloris marina MBIC11017
J. Bacteriol.
198
1393-1400
2016
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2
1
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4
4
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728467
Ito
Evolution of a new chlorophyll ...
Synechocystis sp.
Plant Cell Physiol.
55
593-603
2014
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736444
Harada
Chlorophyllide a oxidoreductas ...
no activity in Rhodobacter sphaeroides, Rhodopseudomonas palustris, Rhodopseudomonas palustris J1002
J. Biol. Chem.
289
12716-12726
2014
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3
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737646
Canniffe
Elucidation of the preferred r ...
Synechocystis sp.
Biochem. J.
462
433-440
2014
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744270
Canniffe
Elucidation of the preferred ...
Synechocystis sp. PCC 6803
Biochem. J.
462
433-440
2014
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3
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3
3
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746037
Ito
Evolution of a new chlorophyl ...
Synechocystis sp. PCC 6803
Plant Cell Physiol.
55
593-603
2014
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1
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5
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2
2
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737687
Saunders
Characterization of BciB: a fe ...
Chloroherpeton thalassium
Biochemistry
52
8442-8451
2013
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1
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1
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8
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744314
Saunders
Characterization of BciB a fe ...
Chloroherpeton thalassium, Chloroherpeton thalassium ATCC 35110
Biochemistry
52
8442-8451
2013
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1
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9
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1
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738520
Liu
Multiple types of 8-vinyl redu ...
Chlorobium ferrooxidans, Chlorobium ferrooxidans DSM 13031, Chlorobium limicola, Chlorobium limicola DSM 245, Chlorobium phaeobacteroides, Chlorobium phaeobacteroides DSM 266, Chloroherpeton thalassium, Chloroherpeton thalassium ATCC 35110, Pelodictyon clathratiforme, Pelodictyon clathratiforme DSM 5477, Prosthecochloris aestuarii, Prosthecochloris aestuarii DSM 271
J. Bacteriol.
193
4996-4998
2011
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