Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21DE3 Star cells | Mycoplasma capricolum subsp. capricolum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil17 in tRNA + NADPH + H+ | Mycoplasma capricolum subsp. capricolum | - |
5,6-dihydrouracil17 in tRNA + NADP+ | - |
? | |
uracil17 in tRNA + NADPH + H+ | Mycoplasma capricolum subsp. capricolum ATCC 14323 | - |
5,6-dihydrouracil17 in tRNA + NADP+ | - |
? | |
uracil20 in tRNA + NADPH + H+ | Mycoplasma capricolum subsp. capricolum | - |
5,6-dihydrouracil20 in tRNA + NADP+ | - |
? | |
uracil20 in tRNA + NADPH + H+ | Mycoplasma capricolum subsp. capricolum ATCC 14323 | - |
5,6-dihydrouracil20 in tRNA + NADP+ | - |
? | |
uracil20a in tRNA + NADPH + H+ | Mycoplasma capricolum subsp. capricolum | - |
5,6-dihydrouracil20a in tRNA + NADP+ | - |
? | |
uracil20a in tRNA + NADPH + H+ | Mycoplasma capricolum subsp. capricolum ATCC 14323 | - |
5,6-dihydrouracil20a in tRNA + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycoplasma capricolum subsp. capricolum | A0A084EMY4 | - |
- |
Mycoplasma capricolum subsp. capricolum ATCC 14323 | A0A084EMY4 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap column chromatography and Superdex S200 gel filtration | Mycoplasma capricolum subsp. capricolum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
more the enzyme it is not able to synthetize uracil16 in tRNA | - |
Mycoplasma capricolum subsp. capricolum | ? | - |
? | |
more the enzyme it is not able to synthetize uracil16 in tRNA | - |
Mycoplasma capricolum subsp. capricolum ATCC 14323 | ? | - |
? | |
uracil in bulk tRNA + NADH + H+ | the enzyme exhibits a clear preference for NADPH compared to NADH | Mycoplasma capricolum subsp. capricolum | 5,6-dihydrouracil in bulk tRNA + NAD+ | - |
? | |
uracil in bulk tRNA + NADH + H+ | the enzyme exhibits a clear preference for NADPH compared to NADH | Mycoplasma capricolum subsp. capricolum ATCC 14323 | 5,6-dihydrouracil in bulk tRNA + NAD+ | - |
? | |
uracil in bulk tRNA + NADPH + H+ | the enzyme exhibits a clear preference for NADPH compared to NADH | Mycoplasma capricolum subsp. capricolum | 5,6-dihydrouracil in bulk tRNA + NADP+ | - |
? | |
uracil17 in tRNA + NADPH + H+ | - |
Mycoplasma capricolum subsp. capricolum | 5,6-dihydrouracil17 in tRNA + NADP+ | - |
? | |
uracil17 in tRNA + NADPH + H+ | - |
Mycoplasma capricolum subsp. capricolum ATCC 14323 | 5,6-dihydrouracil17 in tRNA + NADP+ | - |
? | |
uracil20 in tRNA + NADPH + H+ | - |
Mycoplasma capricolum subsp. capricolum | 5,6-dihydrouracil20 in tRNA + NADP+ | - |
? | |
uracil20 in tRNA + NADPH + H+ | - |
Mycoplasma capricolum subsp. capricolum ATCC 14323 | 5,6-dihydrouracil20 in tRNA + NADP+ | - |
? | |
uracil20a in tRNA + NADPH + H+ | - |
Mycoplasma capricolum subsp. capricolum | 5,6-dihydrouracil20a in tRNA + NADP+ | - |
? | |
uracil20a in tRNA + NADPH + H+ | - |
Mycoplasma capricolum subsp. capricolum ATCC 14323 | 5,6-dihydrouracil20a in tRNA + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 32000, SDS-PAGE | Mycoplasma capricolum subsp. capricolum |
Synonyms | Comment | Organism |
---|---|---|
dihydrouridine synthase | - |
Mycoplasma capricolum subsp. capricolum |
DusB | - |
Mycoplasma capricolum subsp. capricolum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Mycoplasma capricolum subsp. capricolum | |
NADPH | most effective cofactor | Mycoplasma capricolum subsp. capricolum |