Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
vapor diffusion method, using 1.6 M lithium sulfate and 100 mM HEPES pH 7.5 | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 2 mM used in assay conditions | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil20 in tRNA + NADH + H+ | Escherichia coli | - |
5,6-dihydrouracil20 in tRNA + NAD+ | - |
? | |
uracil20a in tRNA + NADH + H+ | Escherichia coli | - |
5,6-dihydrouracil20a in tRNA + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P32695 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil20 in tRNA + NADH + H+ | - |
Escherichia coli | 5,6-dihydrouracil20 in tRNA + NAD+ | - |
? | |
uracil20a in tRNA + NADH + H+ | - |
Escherichia coli | 5,6-dihydrouracil20a in tRNA + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DusA | isoform | Escherichia coli |
tRNA-dihydrouridine synthases | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Escherichia coli | |
NADPH | - |
Escherichia coli |