Cloned (Comment) | Organism |
---|---|
gene fabI, overexpression in Escherichia coli strain M15 | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
curcumin | i.e. (E,E)-1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione, an uncompetitive inhibition of FabI, shows antibacterial activity against Escherichia coli, sensitivity is decreased in FabI-overexpressing Escherichia coli | Escherichia coli | |
genistein | i.e. 4',5,7-trihydroxyisoflavone | Escherichia coli | |
imperatorin | i.e. 9-[(3-methyl-2-buten-1-yl)oxy]-7H-furo[3,2-g][1]benzopyran-7-one | Escherichia coli | |
luteolin | i.e. 3',4',5,7-tetrahydroxyflavone, uncompetitive inhibition of FabI, sensitivity is decreased in FabI-overexpressing Escherichia coli | Escherichia coli | |
additional information | MMIC50 values of the natural inhibitors, overview | Escherichia coli | |
polydatin | i.e. 3,4',5-trihydroxystilbene-3-beta-D-glucopyranoside | Escherichia coli | |
triclosan | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AEK4 | gene fabI | - |
Escherichia coli MG1655 | P0AEK4 | gene fabI | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | activity detection via measurement of NADPH production by reduction of crotonoyl-CoA | Escherichia coli | ? | - |
? | |
additional information | activity detection via measurement of NADPH production by reduction of crotonoyl-CoA | Escherichia coli MG1655 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
enoyl-ACP reductase | - |
Escherichia coli |
FabI | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics, overview | Escherichia coli | |
0.0071 | - |
luteolin | pH 7.5, 22°C | Escherichia coli | |
0.015 | - |
curcumin | pH 7.5, 22°C | Escherichia coli |