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Literature summary for 1.3.1.88 extracted from

  • Xing, F.; Martzen, M.R.; Phizicky, E.M.
    A conserved family of Saccharomyces cerevisiae synthases effects dihydrouridine modification of tRNA (2002), RNA, 8, 370-381.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
uracil17 in tRNA + NAD(P)H + H+ Saccharomyces cerevisiae
-
5,6-dihydrouracil17 in tRNA + NAD(P)+
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P53759
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
uracil17 in pre-tRNAPhe + NAD(P)H + H+
-
Saccharomyces cerevisiae 5,6-dihydrouracil17 in pre-tRNAPhe + NAD(P)+
-
?
uracil17 in tRNA + NAD(P)H + H+
-
Saccharomyces cerevisiae 5,6-dihydrouracil17 in tRNA + NAD(P)+
-
?

Synonyms

Synonyms Comment Organism
Dus1p
-
Saccharomyces cerevisiae
tRNA-dihydrouridine synthase 1
-
Saccharomyces cerevisiae

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
FAD stimulates activity 25fold Saccharomyces cerevisiae

General Information

General Information Comment Organism
malfunction yeast extract from a dus1-DELTA strain is completely defective in modification of yeast pre-tRNAPhe Saccharomyces cerevisiae