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Literature summary for 1.3.1.77 extracted from

  • Tan, L.; Wang, M.; Kang, Y.; Azeem, F.; Zhou, Z.; Tuo, D.; Maria Preciado Rojo, L.; Khan, I.A.; Pan, Z.
    Biochemical and functional characterization of anthocyanidin reductase (ANR) from Mangifera indica L. (2018), Molecules, 23, 2876 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.77

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) pLysS cells and Arabidopsis thaliana Mangifera indica

Inhibitors

Inhibitors Comment Organism Structure
additional information NaCl concentrations up to 600 mM do not cause any inhibitory effect on the activity of the enzyme; NaCl concentrations up to 600 mM do not cause any inhibitory effect on the activity of the enzyme Mangifera indica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01814
-
 NADPH at pH 7.0 and 37°C Mangifera indica
0.01898
-
 NADPH at pH 7.0 and 37°C Mangifera indica
0.08043
-
 cyanidin at pH 7.0 and 37°C Mangifera indica
0.1164
-
 cyanidin at pH 7.0 and 37°C Mangifera indica

Organism

Organism UniProt Comment Textmining
Mangifera indica A0A3Q8GZM8
-
-
Mangifera indica A0A3S7R533
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Mangifera indica

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Mangifera indica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyanidin + 2 NADPH + H+
-
 Mangifera indica epicatechin + catechin + 2 NADP+
-
 ?
additional information the enzyme is unable to use delphinidin and perlargonidin as a substrate and does not use NADH as cosubstrate Mangifera indica ?
-
-

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Mangifera indica
? x * 36304, calculated from amino acid sequence Mangifera indica
? x * 36339, calculated from amino acid sequence Mangifera indica

Synonyms

Synonyms Comment Organism
ANR1-2
-
 Mangifera indica
ANR1-3
-
 Mangifera indica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
32.3
-
 cyanidin at pH 7.0 and 37°C Mangifera indica
42.37
-
 cyanidin at pH 7.0 and 37°C Mangifera indica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 in citrate buffer Mangifera indica

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Mangifera indica

pI Value

Organism Comment pI Value Maximum pI Value
Mangifera indica calculated from amino acid sequence
-
 5.5
Mangifera indica calculated from amino acid sequence
-
 5.7

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
363
-
 cyanidin at pH 7.0 and 37°C Mangifera indica
401
-
 cyanidin at pH 7.0 and 37°C Mangifera indica