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Literature summary for 1.3.1.75 extracted from

  • Canniffe, D.P.; Chidgey, J.W.; Hunter, C.N.
    Elucidation of the preferred routes of C8-vinyl reduction in chlorophyll and bacteriochlorophyll biosynthesis (2014), Biochem. J., 462, 433-440 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
gene bciA Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
8-vinyl protochlorophyllide a + NADPH + H+ Cereibacter sphaeroides is reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide protochlorophyllide a + NADP+
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8-vinyl protochlorophyllide a + NADPH + H+ Cereibacter sphaeroides DSM 158 is reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide protochlorophyllide a + NADP+
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C8-vinyl chlorophyllide a + NADPH + H+ Cereibacter sphaeroides 8V Chlide is the preferred substrate for BciA, with high substrate specificity chlorophyllide a + NADP+
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 ?
C8-vinyl chlorophyllide a + NADPH + H+ Cereibacter sphaeroides DSM 158 8V Chlide is the preferred substrate for BciA, with high substrate specificity chlorophyllide a + NADP+
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Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides Q3IXP7
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Cereibacter sphaeroides DSM 158 Q3IXP7
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8-vinyl protochlorophyllide a + NADPH + H+ is reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide Cereibacter sphaeroides protochlorophyllide a + NADP+
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8-vinyl protochlorophyllide a + NADPH + H+ is reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyllide Cereibacter sphaeroides DSM 158 protochlorophyllide a + NADP+
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C8-vinyl chlorophyllide a + NADPH + H+ 8V Chlide is the preferred substrate for BciA, with high substrate specificity Cereibacter sphaeroides chlorophyllide a + NADP+
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 ?
C8-vinyl chlorophyllide a + NADPH + H+ preferred substrate with high substrate specificity Cereibacter sphaeroides chlorophyllide a + NADP+
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 ?
C8-vinyl chlorophyllide a + NADPH + H+ 8V Chlide is the preferred substrate for BciA, with high substrate specificity Cereibacter sphaeroides DSM 158 chlorophyllide a + NADP+
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 ?
C8-vinyl chlorophyllide a + NADPH + H+ preferred substrate with high substrate specificity Cereibacter sphaeroides DSM 158 chlorophyllide a + NADP+
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 ?

Synonyms

Synonyms Comment Organism
8V reductase
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 Cereibacter sphaeroides
8VR
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 Cereibacter sphaeroides
BciA
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 Cereibacter sphaeroides
C8-vinyl reductase
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 Cereibacter sphaeroides
nucleoside-diphosphate-sugar epimerase UniProt Cereibacter sphaeroides
RSP_3070
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 Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
NADPH
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 Cereibacter sphaeroides

Expression

Organism Comment Expression
Cereibacter sphaeroides generation of an enzyme BciA knockout mutant and recombinant expression of Synechocystis sp. PCC6803 gene bciB in the mutant, gene bciB is unable to restore the accumulation of pigments containing groups reduced at the C8 position additional information

General Information

General Information Comment Organism
evolution two unrelated classes of C8-vinyl reductase are known to exist, BciA and BciB Cereibacter sphaeroides
metabolism the majority of (B)Chls utilized for light-harvesting carry an ethyl group at the C8 position (8E) of the macrocycle. This group is produced by the reduction of a vinyl group (8V), catalysed by an 8V reductase, 8VR, resulting in the production of an 8E pigment. Although BciA demonstrates flexible specificity, biosynthetic heterogeneity does not occur in this organism when its preferred substrate is present, implying that, in this case, the biosynthetic pathway is linear Cereibacter sphaeroides