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Literature summary for 1.3.1.75 extracted from

  • Kolossov, V.L.; Rebeiz, C.A.
    Chloroplast biogenesis 84: solubilization and partial purification of membrane-bound [4-vinyl]chlorophyllide a reductase from etiolated barley leaves (2001), Anal. Biochem., 295, 214-219.
    View publication on PubMed
Search for more literature for 1.3.1.75

General Stability

General Stability Organism
glycerol stabilizes the membrane-bound enzyme, it has no effect on the solubilized enzyme Hordeum vulgare

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Hordeum vulgare 9507
-
chloroplast
-
 Cucumis sativus 9507
-
etioplast
-
 Hordeum vulgare 9513
-
etioplast
-
 Cucumis sativus 9513
-
membrane
-
 Hordeum vulgare 16020
-
membrane
-
 Cucumis sativus 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,4-divinyl chlorophyllide a + NADPH + H+ Hordeum vulgare key enzyme of the chlorophyll biosynthetic pathway 2-vinyl-4-ethyl chlorophyllide a + NADP+ i.e. monovinyl chlorophyllide a r
2,4-divinyl chlorophyllide a + NADPH + H+ Cucumis sativus key enzyme of the chlorophyll biosynthetic pathway 2-vinyl-4-ethyl chlorophyllide a + NADP+ i.e. monovinyl chlorophyllide a r

Organism

Organism UniProt Comment Textmining
Cucumis sativus
-
 var. Poinsett 76
-
Hordeum vulgare
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Hordeum vulgare

Source Tissue

Source Tissue Comment Organism Textmining
leaf etiolated Hordeum vulgare
-
leaf etiolated Cucumis sativus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.17
-
 partially purified enzyme Hordeum vulgare

Storage Stability

Storage Stability Organism
-80 °C, partially purified enzyme, stable for several months Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-divinyl chlorophyllide a + NADPH + H+
-
 Hordeum vulgare 2-vinyl-4-ethyl chlorophyllide a + NADP+ i.e. monovinyl chlorophyllide a r
2,4-divinyl chlorophyllide a + NADPH + H+
-
 Cucumis sativus 2-vinyl-4-ethyl chlorophyllide a + NADP+ i.e. monovinyl chlorophyllide a r
2,4-divinyl chlorophyllide a + NADPH + H+ key enzyme of the chlorophyll biosynthetic pathway Hordeum vulgare 2-vinyl-4-ethyl chlorophyllide a + NADP+ i.e. monovinyl chlorophyllide a r
2,4-divinyl chlorophyllide a + NADPH + H+ key enzyme of the chlorophyll biosynthetic pathway Cucumis sativus 2-vinyl-4-ethyl chlorophyllide a + NADP+ i.e. monovinyl chlorophyllide a r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3
-
-
 Cucumis sativus
6.3
-
 assay at Hordeum vulgare