Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Top10 cells | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
15-keto prostaglandin F2alpha | 43.06% residual activity at 0.1 mM | Rattus norvegicus | |
benzylidene acetophenone | 21.09% residual activity at 0.5 mM | Rattus norvegicus | |
dicoumarol | 11.45% residual activity at 0.1 mM | Rattus norvegicus | |
indomethacin | 14.22% residual activity at 0.1 mM | Rattus norvegicus | |
additional information | finasteride has no inhibitory effect | Rattus norvegicus | |
quercitrin | 13.14% residual activity at 0.1 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0078 | - |
15-ketoprostaglandin E2 | in 100 mM phosphate buffer (pH 7.4), at 25°C | Rattus norvegicus | |
0.0123 | - |
phenyl propenyl ketone | in 100 mM phosphate buffer (pH 7.4), at 25°C | Rattus norvegicus | |
0.0277 | - |
benzylidene acetone | in 100 mM phosphate buffer (pH 7.4), at 25°C | Rattus norvegicus | |
0.0791 | - |
cinnamoylthiophene | in 100 mM phosphate buffer (pH 7.4), at 25°C | Rattus norvegicus | |
0.1289 | - |
Methyl vinyl ketone | in 100 mM phosphate buffer (pH 7.4), at 25°C | Rattus norvegicus | |
0.1399 | - |
benzylidene acetonphenone | in 100 mM phosphate buffer (pH 7.4), at 25°C | Rattus norvegicus | |
0.461 | - |
4-hydroxy-2-nonenal | in 100 mM phosphate buffer (pH 7.4), at 25°C | Rattus norvegicus | |
1.426 | - |
cinnamaldehyde | in 100 mM phosphate buffer (pH 7.4), at 25°C | Rattus norvegicus | |
2.194 | - |
trans-2-hexenal | in 100 mM phosphate buffer (pH 7.4), at 25°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2',5'-ADP Sepharose 4B column chromatography and HisTrap HP column chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NADPH | - |
Rattus norvegicus | 11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NADP+ | - |
? | |
15-ketoprostaglandin E2 + NADPH | - |
Rattus norvegicus | ? | - |
? | |
4-hydroxy-2-nonenal + NADPH | - |
Rattus norvegicus | ? | - |
? | |
benzylidene acetone + NADPH | - |
Rattus norvegicus | ? | - |
? | |
benzylidene acetonphenone + NADPH | - |
Rattus norvegicus | ? | - |
? | |
cinnamaldehyde + NADPH | - |
Rattus norvegicus | ? | - |
? | |
cinnamoylthiophene + NADPH | - |
Rattus norvegicus | ? | - |
? | |
leukotriene B4 + NADPH | - |
Rattus norvegicus | ? | - |
? | |
methyl vinyl ketone + NADPH | - |
Rattus norvegicus | ? | - |
? | |
phenyl propenyl ketone + NADPH | - |
Rattus norvegicus | ? | - |
? | |
trans-2-hexenal + NADPH | - |
Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
15-ketoprostaglandinDELTA13-reductase | - |
Rattus norvegicus |
bifunctional LTB4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase | a beta-NADPH-dependent alkenal/one oxidoreductase which has a role for leukotriene B4 12-hydroxydehydrogenase/15-ketoprostaglandinDELTA13-reductase | Rattus norvegicus |
PGR | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Rattus norvegicus |