Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Bos taurus | |
p-chloromercuribenzoate | - |
Bos taurus | |
p-chloromercuribenzoate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
NADPH | - |
Bos taurus | |
0.01 | - |
15-ketoprostaglandin E1 | human placenta | Homo sapiens | |
0.03 | - |
15-ketoprostaglandin E1 | bovine lung | Bos taurus | |
0.062 | - |
15-ketoprostaglandin E2 | - |
Bos taurus | |
0.091 | - |
NADH | - |
Bos taurus | |
0.184 | - |
15-ketoprostaglandin F1alpha | - |
Bos taurus | |
0.258 | - |
6,15-diketo-prostaglandin F1alpha | - |
Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
gel filtration | Bos taurus |
68500 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
15-ketoprostaglandin E + NAD(P)H | Gallus gallus | second step in metabolism of E- and Falpha-type prostaglandins | ? | - |
? | |
15-ketoprostaglandin E + NAD(P)H | Homo sapiens | second step in metabolism of E- and Falpha-type prostaglandins | ? | - |
? | |
15-ketoprostaglandin E + NAD(P)H | Bos taurus | second step in metabolism of E- and Falpha-type prostaglandins | ? | - |
? | |
15-oxoprostaglandin F1alpha + NAD(P)H | Gallus gallus | second step in metabolism of E- and Falpha-type prostaglandins | ? | - |
? | |
15-oxoprostaglandin F1alpha + NAD(P)H | Homo sapiens | second step in metabolism of E- and Falpha-type prostaglandins | ? | - |
? | |
15-oxoprostaglandin F1alpha + NAD(P)H | Bos taurus | second step in metabolism of E- and Falpha-type prostaglandins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Gallus gallus | - |
- |
- |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Gallus gallus | - |
lung | - |
Bos taurus | - |
placenta | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.067 | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
-4°C, 0.1 M sodium phosphate, pH 7.4, 1 mM EDTA, 1 mM mercaptoethanol, stable for more than 1 month | Bos taurus |
-80°C, stable for more than 1 year | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H | - |
Gallus gallus | (5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ | - |
ir | |
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H | - |
Homo sapiens | (5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ | - |
ir | |
(5Z,13E)-11alpha-hydroxy-9,15-dioxoprost-5,13-dienoate + NAD(P)H | - |
Bos taurus | (5Z)-11alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ | - |
ir | |
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H | i.e. 15-ketoprostaglandin E2 | Homo sapiens | 11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+ | - |
? | |
11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NAD(P)H | i.e. 15-ketoprostaglandin E2 | Bos taurus | 11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NAD(P)+ | - |
? | |
15-ketoprostaglandin E + NAD(P)H | second step in metabolism of E- and Falpha-type prostaglandins | Gallus gallus | ? | - |
? | |
15-ketoprostaglandin E + NAD(P)H | second step in metabolism of E- and Falpha-type prostaglandins | Homo sapiens | ? | - |
? | |
15-ketoprostaglandin E + NAD(P)H | second step in metabolism of E- and Falpha-type prostaglandins | Bos taurus | ? | - |
? | |
15-ketoprostaglandin E1 + NADH + H+ | - |
Bos taurus | 13,14-dihydro-15-keto-prostaglandin E1 + NAD+ | - |
r | |
15-ketoprostaglandin E1 + NADPH | - |
Homo sapiens | ? | - |
? | |
15-ketoprostaglandin E1 + NADPH | - |
Bos taurus | ? | - |
? | |
15-ketoprostaglandin F1alpha + NAD(P)H | - |
Bos taurus | ? | - |
? | |
15-oxoprostaglandin F1alpha + NAD(P)H | second step in metabolism of E- and Falpha-type prostaglandins | Gallus gallus | ? | - |
? | |
15-oxoprostaglandin F1alpha + NAD(P)H | second step in metabolism of E- and Falpha-type prostaglandins | Homo sapiens | ? | - |
? | |
15-oxoprostaglandin F1alpha + NAD(P)H | second step in metabolism of E- and Falpha-type prostaglandins | Bos taurus | ? | - |
? | |
6,15-diketo-prostaglandin F1alpha + NAD(P)H | - |
Bos taurus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | - |
Homo sapiens | |
NAD(P)H | bovine lung: enzyme can use both NADPH and NADH | Bos taurus | |
NADH | human placenta: enzyme can use only NADH | Homo sapiens |