Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is inhibited by sulfhydryl compounds, e.g. cysteine at concentrations equivalent to 3-nitroacrylic acid, inhibition probably due to the reaction of the sulfhydryl compounds with 3-nitroacrylic acid rather than an inhibition of the enzyme itself | Penicillium atrovenetum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
NADPH | - |
Penicillium atrovenetum | |
0.16 | - |
3-Nitroacrylate | - |
Penicillium atrovenetum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-nitroacrylate + NADPH | Penicillium atrovenetum | biosynthesis of beta-nitropropionic acid | 3-nitropropanoate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium atrovenetum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Penicillium atrovenetum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Penicillium atrovenetum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.866 | - |
- |
Penicillium atrovenetum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-nitroacrylate + NADPH | highly specific | Penicillium atrovenetum | 3-nitropropanoate + NADP+ | - |
? | |
3-nitroacrylate + NADPH | biosynthesis of beta-nitropropionic acid | Penicillium atrovenetum | 3-nitropropanoate + NADP+ | - |
? | |
additional information | no substrates are acrylic acid, crotonic acid, fumaric acid, cinnamic acid, 2-hydroxy-3-nitropropionic acid, shikimic acid, aconitic acid, itaconic acid, pyruvic acid, phosphoenolpyruvate, cytochrome c | Penicillium atrovenetum | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Penicillium atrovenetum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | at pH 3 about 35% of activity maximum, at pH 7 about 60% of activity maximum | Penicillium atrovenetum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Penicillium atrovenetum | |
NADPH | NADH cannot replace NADPH | Penicillium atrovenetum |