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Literature summary for 1.3.1.123 extracted from

  • Kries, H.; Kellner, F.; Kamileen, M.; O'Connor, S.
    Inverted stereocontrol of iridoid synthase in snapdragon (2017), J. Biol. Chem., 292, 14659-14667 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.1.123

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli soluBL21 (DE3) cells Antirrhinum majus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0011
-
 (6E)-8-oxogeranial with NADPH as cosubstrate, at pH 7.0 and 25°C Antirrhinum majus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(6E)-8-oxogeranial + NADPH + H+ Antirrhinum majus
-
 (R)-8-oxocitronellal + (S)-8-oxocitronellal + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Antirrhinum majus
-
 snapdragon
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography and Superdex S200 gel filtration Antirrhinum majus

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Antirrhinum majus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6E)-8-oxogeranial + NADPH + H+
-
 Antirrhinum majus (R)-8-oxocitronellal + (S)-8-oxocitronellal + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Antirrhinum majus

Synonyms

Synonyms Comment Organism
iridoid synthase
-
 Antirrhinum majus
ISY
-
 Antirrhinum majus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.72
-
 (6E)-8-oxogeranial with NADPH as cosubstrate, at pH 7.0 and 25°C Antirrhinum majus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Antirrhinum majus