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Literature summary for 1.3.1.111 extracted from

  • Harada, J.; Miyago, S.; Mizoguchi, T.; Azai, C.; Inoue, K.; Tamiaki, H.; Oh-oka, H.
    Accumulation of chlorophyllous pigments esterified with the geranylgeranyl group and photosynthetic competence in the CT2256-deleted mutant of the green sulfur bacterium Chlorobium tepidum (2008), Photochem. Photobiol. Sci., 7, 1179-1187.
    View publication on PubMed
Search for more literature for 1.3.1.111

Cloned(Commentary)

Cloned (Comment) Organism
gene CT2256, recombinant expression in Rhodobacter capsulatus mutant strain DB391 defective in the bchP gene and partial complementation Chlorobaculum tepidum

Protein Variants

Protein Variants Comment Organism
additional information generation of a gene CT2256 deletion mutant, which almost shows no apparent phenotype compared to the wild-type. The purple bacterium Rhodobacter capsulatus mutant strain DB391 defective in the bchP gene is partially complemented with the CT2256 gene, bacteriochlorophyllide a is synthesized in the mutant in addition to accumulating other intermediates, phenotype, overview Chlorobaculum tepidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetics Chlorobaculum tepidum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
bacteriochlorophyll a + 3 NADP+ Chlorobaculum tepidum
-
 geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
 ?
bacteriochlorophyll a + 3 NADP+ Chlorobaculum tepidum WT2321
-
 geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Chlorobaculum tepidum Q8KAB0 gene CT2256
-
Chlorobaculum tepidum WT2321 Q8KAB0 gene CT2256
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bacteriochlorophyll a + 3 NADP+
-
 Chlorobaculum tepidum geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
 ?
bacteriochlorophyll a + 3 NADP+
-
 Chlorobaculum tepidum WT2321 geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Chlorobaculum tepidum

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Chlorobaculum tepidum

General Information

General Information Comment Organism
malfunction the CT2256-deleted mutant shows accumulation of chlorophyllous pigments esterified with the geranylgeranyl group and photosynthetic competence, phenotype, overview Chlorobaculum tepidum
physiological function the enzyme is involved esterification of bacteriochlorophyll a with DELTA2,6-phytadienol and phytol, respectively, which is produced by reduction of the geranylgeranyl group at the C-17 propionate residue Chlorobaculum tepidum