BRENDA - Enzyme Database
show all sequences of 1.3.1.111

Accumulation of chlorophyllous pigments esterified with the geranylgeranyl group and photosynthetic competence in the CT2256-deleted mutant of the green sulfur bacterium Chlorobium tepidum

Harada, J.; Miyago, S.; Mizoguchi, T.; Azai, C.; Inoue, K.; Tamiaki, H.; Oh-oka, H.; Photochem. Photobiol. Sci. 7, 1179-1187 (2008)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene CT2256, recombinant expression in Rhodobacter capsulatus mutant strain DB391 defective in the bchP gene and partial complementation
Chlorobaculum tepidum
Engineering
Protein Variants
Commentary
Organism
additional information
generation of a gene CT2256 deletion mutant, which almost shows no apparent phenotype compared to the wild-type. The purple bacterium Rhodobacter capsulatus mutant strain DB391 defective in the bchP gene is partially complemented with the CT2256 gene, bacteriochlorophyllide a is synthesized in the mutant in addition to accumulating other intermediates, phenotype, overview
Chlorobaculum tepidum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
steady-state kinetics
Chlorobaculum tepidum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
bacteriochlorophyll a + 3 NADP+
Chlorobaculum tepidum
-
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
?
bacteriochlorophyll a + 3 NADP+
Chlorobaculum tepidum WT2321
-
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Chlorobaculum tepidum
Q8KAB0
gene CT2256
-
Chlorobaculum tepidum WT2321
Q8KAB0
gene CT2256
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
bacteriochlorophyll a + 3 NADP+
-
736948
Chlorobaculum tepidum
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
-
?
bacteriochlorophyll a + 3 NADP+
-
736948
Chlorobaculum tepidum WT2321
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Chlorobaculum tepidum
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Chlorobaculum tepidum
Cloned(Commentary) (protein specific)
Commentary
Organism
gene CT2256, recombinant expression in Rhodobacter capsulatus mutant strain DB391 defective in the bchP gene and partial complementation
Chlorobaculum tepidum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Chlorobaculum tepidum
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
generation of a gene CT2256 deletion mutant, which almost shows no apparent phenotype compared to the wild-type. The purple bacterium Rhodobacter capsulatus mutant strain DB391 defective in the bchP gene is partially complemented with the CT2256 gene, bacteriochlorophyllide a is synthesized in the mutant in addition to accumulating other intermediates, phenotype, overview
Chlorobaculum tepidum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
steady-state kinetics
Chlorobaculum tepidum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
bacteriochlorophyll a + 3 NADP+
Chlorobaculum tepidum
-
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
?
bacteriochlorophyll a + 3 NADP+
Chlorobaculum tepidum WT2321
-
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
bacteriochlorophyll a + 3 NADP+
-
736948
Chlorobaculum tepidum
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
-
?
bacteriochlorophyll a + 3 NADP+
-
736948
Chlorobaculum tepidum WT2321
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Chlorobaculum tepidum
General Information
General Information
Commentary
Organism
malfunction
the CT2256-deleted mutant shows accumulation of chlorophyllous pigments esterified with the geranylgeranyl group and photosynthetic competence, phenotype, overview
Chlorobaculum tepidum
physiological function
the enzyme is involved esterification of bacteriochlorophyll a with DELTA2,6-phytadienol and phytol, respectively, which is produced by reduction of the geranylgeranyl group at the C-17 propionate residue
Chlorobaculum tepidum
General Information (protein specific)
General Information
Commentary
Organism
malfunction
the CT2256-deleted mutant shows accumulation of chlorophyllous pigments esterified with the geranylgeranyl group and photosynthetic competence, phenotype, overview
Chlorobaculum tepidum
physiological function
the enzyme is involved esterification of bacteriochlorophyll a with DELTA2,6-phytadienol and phytol, respectively, which is produced by reduction of the geranylgeranyl group at the C-17 propionate residue
Chlorobaculum tepidum
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736948
Harada
Accumulation of chlorophyllous ...
Chlorobaculum tepidum, Chlorobaculum tepidum WT2321
Photochem. Photobiol. Sci.
7
1179-1187
2008
-
-
1
-
1
-
-
1
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
2
2
-
-
-
692822
Addlesee
Rhodospirillum rubrum possesse ...
Rhodobacter sphaeroides, Rhodospirillum rubrum
J. Bacteriol.
184
1578-1586
2002
-
-
1
-
2
-
-
-
-
-
-
3
-
6
-
-
-
-
-
-
-
-
8
-
8
-
-
-
-
-
-
-
4
-
-
-
-
-
1
4
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
692821
Addlesee
Physical mapping and functiona ...
Rhodobacter sphaeroides
J. Bacteriol.
181
7248-7255
1999
-
-
1
-
1
-
-
-
-
-
-
2
-
4
-
-
-
-
-
-
-
-
4
-
2
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
736098
Addlesee
Cloning, sequencing and functi ...
Rhodobacter sphaeroides, Rhodobacter sphaeroides NCIB 8253, Synechocystis sp.
FEBS Lett.
389
126-130
1996
-
-
2
-
1
-
-
-
-
-
1
3
-
3
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
2
-
1
-
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
3
1
-
-
-
-
-
-
-
-
-
3
3
-
-
-
735661
Bollivar
Molecular genetic analysis of ...
Rhodobacter capsulatus, Rhodobacter capsulatus SB1003
Biochemistry
33
12763-12768
1994
-
-
1
-
1
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-