BRENDA - Enzyme Database
show all sequences of 1.3.1.111

Molecular genetic analysis of terminal steps in bacteriochlorophyll a biosynthesis: characterization of a Rhodobacter capsulatus strain that synthesizes geranylgeraniol-esterified bacteriochlorophyll a

Bollivar, D.; Biochemistry 33, 12763-12768 (1994)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene bchP, recombinant expression in Escherichia coli and in the plasmid-mobilizing Rhodobacter capsulatus strain TecS
Rhodobacter capsulatus
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of a chromosomal KmR insertion mutation of gene bchP performed by GTA-mediated homologous recombination
Rhodobacter capsulatus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
bacteriochlorophyll a + 3 NADP+
Rhodobacter capsulatus
-
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
?
bacteriochlorophyll a + 3 NADP+
Rhodobacter capsulatus SB1003
-
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodobacter capsulatus
Q9Z5D4
gene bchP
-
Rhodobacter capsulatus SB1003
Q9Z5D4
gene bchP
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
bacteriochlorophyll a + 3 NADP+
-
735661
Rhodobacter capsulatus
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
-
?
bacteriochlorophyll a + 3 NADP+
three reductive steps, overview
735661
Rhodobacter capsulatus
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
-
?
bacteriochlorophyll a + 3 NADP+
-
735661
Rhodobacter capsulatus SB1003
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
-
?
bacteriochlorophyll a + 3 NADP+
three reductive steps, overview
735661
Rhodobacter capsulatus SB1003
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Rhodobacter capsulatus
Cloned(Commentary) (protein specific)
Commentary
Organism
gene bchP, recombinant expression in Escherichia coli and in the plasmid-mobilizing Rhodobacter capsulatus strain TecS
Rhodobacter capsulatus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Rhodobacter capsulatus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of a chromosomal KmR insertion mutation of gene bchP performed by GTA-mediated homologous recombination
Rhodobacter capsulatus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
bacteriochlorophyll a + 3 NADP+
Rhodobacter capsulatus
-
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
?
bacteriochlorophyll a + 3 NADP+
Rhodobacter capsulatus SB1003
-
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
bacteriochlorophyll a + 3 NADP+
-
735661
Rhodobacter capsulatus
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
-
?
bacteriochlorophyll a + 3 NADP+
three reductive steps, overview
735661
Rhodobacter capsulatus
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
-
?
bacteriochlorophyll a + 3 NADP+
-
735661
Rhodobacter capsulatus SB1003
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
-
?
bacteriochlorophyll a + 3 NADP+
three reductive steps, overview
735661
Rhodobacter capsulatus SB1003
geranylgeranyl bacteriochlorophyllide a + 3 NADPH + 3 H+
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
a mutant in orf391 synthesizes bacteriochlorophyll a that is esterified with geranylgeraniol rather than the normal phytol. Strains that accumulate geranylgeraniol-esterified bacteriochlorophyll a exhibit reduced photosynthetic growth capability compared to that observed with the parent strain which synthesizes bacteriochlorophyll a that is esterified with phytol. But synthesis of geranylgeranyl bacteriochlorophyllide a does not per se impair function of the reaction center complex nor energy transfer from the light harvesting complex
Rhodobacter capsulatus
metabolism
esterification of bacteriochlorophyllide a initially involves the addition of a geranylgeraniol group followed by sequential reduction of the geranylgeraniol moiety tophytol which is the end product of the pathway. Synthesis of geranylgeraniol-esterified bacteriochlorophyll does not affect the energy transfer rate from light harvesting to reaction center complexes nor the electron transfer function as measured by the yield of electron transfer to the primary and secondary quinones, the charge recombination rate from the quinones, and the rate of cytochrome c2 oxidation. Pathway for synthesis of bacteriochlorophyll from bacteriochlorophyllide, overview
Rhodobacter capsulatus
General Information (protein specific)
General Information
Commentary
Organism
malfunction
a mutant in orf391 synthesizes bacteriochlorophyll a that is esterified with geranylgeraniol rather than the normal phytol. Strains that accumulate geranylgeraniol-esterified bacteriochlorophyll a exhibit reduced photosynthetic growth capability compared to that observed with the parent strain which synthesizes bacteriochlorophyll a that is esterified with phytol. But synthesis of geranylgeranyl bacteriochlorophyllide a does not per se impair function of the reaction center complex nor energy transfer from the light harvesting complex
Rhodobacter capsulatus
metabolism
esterification of bacteriochlorophyllide a initially involves the addition of a geranylgeraniol group followed by sequential reduction of the geranylgeraniol moiety tophytol which is the end product of the pathway. Synthesis of geranylgeraniol-esterified bacteriochlorophyll does not affect the energy transfer rate from light harvesting to reaction center complexes nor the electron transfer function as measured by the yield of electron transfer to the primary and secondary quinones, the charge recombination rate from the quinones, and the rate of cytochrome c2 oxidation. Pathway for synthesis of bacteriochlorophyll from bacteriochlorophyllide, overview
Rhodobacter capsulatus
Other publictions for EC 1.3.1.111
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736948
Harada
Accumulation of chlorophyllous ...
Chlorobaculum tepidum, Chlorobaculum tepidum WT2321
Photochem. Photobiol. Sci.
7
1179-1187
2008
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1
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1
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1
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2
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2
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1
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1
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1
1
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1
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1
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2
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2
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1
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2
2
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692822
Addlesee
Rhodospirillum rubrum possesse ...
Rhodobacter sphaeroides, Rhodospirillum rubrum
J. Bacteriol.
184
1578-1586
2002
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1
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2
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3
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6
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8
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4
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1
4
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2
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3
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8
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3
3
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692821
Addlesee
Physical mapping and functiona ...
Rhodobacter sphaeroides
J. Bacteriol.
181
7248-7255
1999
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1
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1
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4
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1
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1
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4
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1
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1
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3
3
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736098
Addlesee
Cloning, sequencing and functi ...
Rhodobacter sphaeroides, Rhodobacter sphaeroides NCIB 8253, Synechocystis sp.
FEBS Lett.
389
126-130
1996
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2
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1
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1
3
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3
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3
1
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2
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2
2
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1
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1
3
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3
1
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3
3
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735661
Bollivar
Molecular genetic analysis of ...
Rhodobacter capsulatus, Rhodobacter capsulatus SB1003
Biochemistry
33
12763-12768
1994
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1
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2
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4
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1
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1
1
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1
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4
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2
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