Literature summary for 1.3.1.104 extracted from

Ling, B.; Li, H.; Yan, L.; Liu, R.; Liu, Y.
Conversion mechanism of enoyl thioesters into acyl thioesters catalyzed by 2-enoyl-thioester reductases from Candida tropicalis (2019), Phys. Chem. Chem. Phys., 21, 10105-10113 .

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Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Candida tropicalis	5739	-

Organism

Organism	UniProt	Comment	Textmining
Candida tropicalis	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
crotonyl-CoA + NADPH + H+	-	Candida tropicalis	butanoyl-CoA + NADP+	-	?

Synonyms

Synonyms	Comment	Organism
ETR1	-	Candida tropicalis

General Information

General Information	Comment	Organism
metabolism	the whole reaction involves the formation of the covalent ene adduct and the proton transfer from Tyr79 to the substrate. In the first step the hydride of NADPH easily transfers to crotonyl-CoA to generate the substrate carbanion if the hydroxyl of Tyr79 forms a hydrogen bond chain with Ser70 and crystal water molecules. Then, the carbanion and NADP+ form a stable covalent ene adduct. The formation of the ene adduct follows the Michael addition mechanism rather than the electrocyclic ene reactions. The final electrophilic attack of the Tyr79 proton on C8 of the substrate affords the product of acyl thioester. The key crystal water molecules do not directly participate in the catalytic reactions, but they play a role in maintaining the relative stability of the substrate to NADPH	Candida tropicalis

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Release 2023.1 (January 2023)