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Literature summary for 1.21.3.10 extracted from

  • Hu, W.; Song, H.; Sae Her, A.; Bak, D.W.; Naowarojna, N.; Elliott, S.J.; Qin, L.; Chen, X.; Liu, P.
    Bioinformatic and biochemical characterizations of C-S bond formation and cleavage enzymes in the fungus Neurospora crassa ergothioneine biosynthetic pathway (2014), Org. Lett., 16, 5382-5385.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.21.3.10

Application

Application Comment Organism
biotechnology the Neurospora crassa ergothioneine biosynthetic pathway may be a more suitable platform than the mycobacterial one for ergothioneine production through metabolic engineering because the ergothioneine and glutathione biosynthetic pathways are uncoupled and they do not compete with each other anymore in Neurospora crassa Neurospora crassa

Cloned(Commentary)

Cloned (Comment) Organism
gene egt1, overexpression in Escherichia coli strain BL21(DE3) from pASK-IBA3+ vector Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.39
-
 hercynine with gamma-L-glutamyl-L-cysteine, pH and temperature not specified in the publication Neurospora crassa
0.436
-
 hercynine with L-Cys, pH and temperature not specified in the publication Neurospora crassa
0.603
-
 L-cysteine pH and temperature not specified in the publication Neurospora crassa
7.68
-
 gamma-L-glutamyl-L-cysteine pH and temperature not specified in the publication Neurospora crassa

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ a non-heme iron enzyme, that contains about 0.90 Fe per Egt1 monomer Neurospora crassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hercynine + L-cysteine + O2 Neurospora crassa
-
 S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
hercynine + L-cysteine + O2 Neurospora crassa ATCC 24698
-
 S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Neurospora crassa Q7RX33 gene egt1
-
Neurospora crassa ATCC 24698 Q7RX33 gene egt1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hercynine + gamma-L-glutamyl-L-cysteine + O2 reaction of EC 1.14.99.50, enzyme Egt1 has about 62fold greater specificity (kobs/Km) for L-cysteine relative to gamma-Glu-Cys Neurospora crassa gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
hercynine + gamma-L-glutamyl-L-cysteine + O2 reaction of EC 1.14.99.50, enzyme Egt1 has about 62fold greater specificity (kobs/Km) for L-cysteine relative to gamma-Glu-Cys Neurospora crassa ATCC 24698 gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
hercynine + L-cysteine + O2
-
 Neurospora crassa S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
hercynine + L-cysteine + O2
-
 Neurospora crassa ATCC 24698 S-(hercyn-2-yl)-L-cysteine S-oxide + H2O
-
 ?
additional information usage of three different assays, when histidine and cysteine are the substrates (cf. EC 1.14.99.52), kobs is at least 100fold less than the case using the Cys and hercynine combination, and when gamma-Glu-Cys and hercynine are the substrates (cf. EC 1.14.99.50), the activity is low compared to the native substrates, and when gamma-Glu-Cys and histidine are the substrates, similar to the case of histidine and cysteine combination, the rate is close to background level, overview Neurospora crassa ?
-
 ?
additional information usage of three different assays, when histidine and cysteine are the substrates (cf. EC 1.14.99.52), kobs is at least 100fold less than the case using the Cys and hercynine combination, and when gamma-Glu-Cys and hercynine are the substrates (cf. EC 1.14.99.50), the activity is low compared to the native substrates, and when gamma-Glu-Cys and histidine are the substrates, similar to the case of histidine and cysteine combination, the rate is close to background level, overview Neurospora crassa ATCC 24698 ?
-
 ?

Synonyms

Synonyms Comment Organism
Egt1
-
 Neurospora crassa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.46
-
 hercynine with gamma-L-glutamyl-L-cysteine, pH and temperature not specified in the publication Neurospora crassa
2.27
-
 hercynine with L-Cys, pH and temperature not specified in the publication Neurospora crassa

General Information

General Information Comment Organism
metabolism the enzyme catalyzes a step in the ergothioneine biosynthetic pathway, with the oxidative C-S bond formation and the subsequent C-S bond cleavage reaction, which result in a net transfer of the sulfur atom form cysteine to histidine imidazole side-chain, overview. The fungus Neurospora crassa has a more concise ergothioneine biosynthetic pathway because its nonheme iron enzyme, Egt1, makes use of cysteine instead of gamma-Glu-Cys as the substrate. Such a change of substrate preference eliminates the competition between ergothioneine and glutathione biosyntheses Neurospora crassa