Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.21.3.1 extracted from

  • McNeill, L.A.; Brown, T.J.N.; Sami, M.; Clifton, I.J.; Burzlaff, N.I.; Claridge, T.D.W.; Adlington, R.M.; Baldwin, J.E.; Rutledge, P.J.; Schofield, C.J.
    Terminally truncated isopenicillin N synthase generates a dithioester product evidence for a thioaldehyde intermediate during catalysis and a new mode of reaction for non-heme iron oxidases (2017), Chemistry, 23, 12815-12824 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.21.3.1

Protein Variants

Protein Variants Comment Organism
K98E the mutant shows about 8% of wild type activity Aspergillus nidulans
L223I the mutant shows about 33% of wild type activity Aspergillus nidulans
L223V the mutant shows about 33% of wild type activity Aspergillus nidulans
L231I the mutant shows about 35% of wild type activity Aspergillus nidulans
L231T inactive Aspergillus nidulans
L231V the mutant shows about 25% of wild type activity Aspergillus nidulans
P283A the mutant shows about 12% of wild type activity Aspergillus nidulans
P283I the mutant shows about 10% of wild type activity Aspergillus nidulans
P283L inactive Aspergillus nidulans
P283V the mutant shows about 9% of wild type activity Aspergillus nidulans
V272T the mutant shows about 27% of wild type activity Aspergillus nidulans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ dependent on Aspergillus nidulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 Aspergillus nidulans
-
 isopenicillin N + H2O
-
 ?
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 Aspergillus nidulans ATCC 38163
-
 isopenicillin N + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans P05326
-
-
Aspergillus nidulans ATCC 38163 P05326
-
-

Purification (Commentary)

Purification (Comment) Organism
Sepharose S75 gel filtration and MonoQ resin column chromatography Aspergillus nidulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
delta-(L-alpha-aminoadipoyl)-L-cysteine D-alpha-hydroxyisovaleryl ester + O2
-
 Aspergillus nidulans (2S)-2-amino-6-([(2R)-1-[(1S)-1-carboxy-2-methylpropoxy]-1,3-dioxo-3-sulfanylpropan-2-yl]amino)-6-oxohexanoic acid + H2O
-
 ?
delta-(L-alpha-aminoadipoyl)-L-cysteine D-alpha-hydroxyisovaleryl ester + O2
-
 Aspergillus nidulans ATCC 38163 (2S)-2-amino-6-([(2R)-1-[(1S)-1-carboxy-2-methylpropoxy]-1,3-dioxo-3-sulfanylpropan-2-yl]amino)-6-oxohexanoic acid + H2O
-
 ?
delta-(L-alpha-aminoadipoyl)-L-cysteine D-alpha-hydroxyisovaleryl ester + O2
-
 Aspergillus nidulans ?
-
 ?
delta-(L-alpha-aminoadipoyl)-L-cysteine D-alpha-hydroxyisovaleryl ester + O2
-
 Aspergillus nidulans ATCC 38163 ?
-
 ?
L-delta-(alpha-aminoadipoyl)-L-cysteinyl-glycine + O2
-
 Aspergillus nidulans (2S)-2-amino-6-([(2S)-1-[(carboxymethyl)amino]-3,3-dihydroxy-1-oxopropan-2-yl]amino)-6-oxohexanoic acid + H2O
-
 ?
L-delta-(alpha-aminoadipoyl)-L-cysteinyl-glycine + O2
-
 Aspergillus nidulans ATCC 38163 (2S)-2-amino-6-([(2S)-1-[(carboxymethyl)amino]-3,3-dihydroxy-1-oxopropan-2-yl]amino)-6-oxohexanoic acid + H2O
-
 ?
L-delta-(alpha-aminoadipoyl)-L-cysteinyl-glycine + O2
-
 Aspergillus nidulans ?
-
 ?
L-delta-(alpha-aminoadipoyl)-L-cysteinyl-glycine + O2
-
 Aspergillus nidulans ATCC 38163 ?
-
 ?
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2
-
 Aspergillus nidulans isopenicillin N + H2O
-
 ?
N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2
-
 Aspergillus nidulans ATCC 38163 isopenicillin N + H2O
-
 ?

Synonyms

Synonyms Comment Organism
IPNS
-
 Aspergillus nidulans
isopenicillin N synthase
-
 Aspergillus nidulans