Application | Comment | Organism |
---|---|---|
synthesis | production of deuterium- or tritium-labeled substances, mutant enzymes could be applied as NADPH regeneration systems | Pseudomonas stutzeri |
Protein Variants | Comment | Organism |
---|---|---|
E175A/A176R | double mutant, 3.6-fold higher efficiency with NAD+, 1000-fold higher efficiency with NADP+, 3-fold favor for NADP+ over NAD+ as cofactor | Pseudomonas stutzeri |
H292F | no activity, NAD+ binding is abolished | Pseudomonas stutzeri |
H292K | no activity, NAD+ binding is abolished | Pseudomonas stutzeri |
H292N | no activity, NAD+ binding is abolished | Pseudomonas stutzeri |
H292Q | no activity, NAD+ binding is abolished | Pseudomonas stutzeri |
R237K | strong increase in Km for both substrates, reduced Vmax | Pseudomonas stutzeri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyl dicarbonate | - |
Pseudomonas stutzeri | |
sulfite | strong inhibitor, competitive with respect to posphonate | Pseudomonas stutzeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphonate + H2O + NAD+ | Alcaligenes faecalis | NADH regeneration system | phosphate + NADH | - |
ir | |
phosphonate + H2O + NAD+ | Pseudomonas stutzeri | NADH regeneration system | phosphate + NADH | - |
ir | |
phosphonate + H2O + NAD+ | Pseudomonas stutzeri WM88 | NADH regeneration system | phosphate + NADH | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alcaligenes faecalis | - |
- |
- |
Pseudomonas stutzeri | - |
- |
- |
Pseudomonas stutzeri WM88 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phosphonate + NAD+ + H2O = phosphate + NADH + H+ | different reaction mechanism discussed | Pseudomonas stutzeri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme does not accept any alternative substrates | Pseudomonas stutzeri | ? | - |
? | |
additional information | enzyme does not accept any alternative substrates | Pseudomonas stutzeri WM88 | ? | - |
? | |
phosphonate + H2O + NAD+ | - |
Alcaligenes faecalis | phosphate + NADH | - |
ir | |
phosphonate + H2O + NAD+ | - |
Pseudomonas stutzeri | phosphate + NADH | - |
ir | |
phosphonate + H2O + NAD+ | NADH regeneration system | Alcaligenes faecalis | phosphate + NADH | - |
ir | |
phosphonate + H2O + NAD+ | NADH regeneration system | Pseudomonas stutzeri | phosphate + NADH | - |
ir | |
phosphonate + H2O + NAD+ | - |
Pseudomonas stutzeri WM88 | phosphate + NADH | - |
ir | |
phosphonate + H2O + NAD+ | NADH regeneration system | Pseudomonas stutzeri WM88 | phosphate + NADH | - |
ir | |
phosphonate + H2O + NADP+ | NADP+ is a very poor hydride acceptor with the wild type enzyme | Pseudomonas stutzeri | phosphate + NADPH | - |
ir | |
phosphonate + H2O + NADP+ | NADP+ is a very poor hydride acceptor with the wild type enzyme | Pseudomonas stutzeri WM88 | phosphate + NADPH | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
PTDH | - |
Alcaligenes faecalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Pseudomonas stutzeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.25 | - |
- |
Pseudomonas stutzeri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Alcaligenes faecalis | |
NAD+ | - |
Pseudomonas stutzeri | |
NADP+ | - |
Pseudomonas stutzeri |