BRENDA - Enzyme Database
show all sequences of 1.2.7.B2

Molybdenum and vanadium do not replace tungsten in the catalytically active forms of the three tungstoenzymes in the hyperthermophilic archaeon Pyrococcus furiosus

Mukund, S.; Adams, M.W.; J. Bacteriol. 178, 163-167 (1996)

Data extracted from this reference:

Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Tungsten
the enzyme from Pyrococcus furiosus uses exclusively tungsten to synthesize the catalytically active forms of formaldehyde ferredoxin oxidoreductase and active molybdenum- or vanadium-containing isoenzymes are not expressed when the cells are grown in the presence of these other metals
Pyrococcus furiosus
Organism
Organism
UniProt
Commentary
Textmining
Pyrococcus furiosus
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
enzyme from vanadium- and molybdenum-grown cells
Pyrococcus furiosus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
formaldehyde + H2O + oxidized benzyl viologen
-
722490
Pyrococcus furiosus
formate + H+ + reduced benzyl viologen
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
FOR
-
Pyrococcus furiosus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
80
-
assay at
Pyrococcus furiosus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
-
assay at
Pyrococcus furiosus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Tungsten
the enzyme from Pyrococcus furiosus uses exclusively tungsten to synthesize the catalytically active forms of formaldehyde ferredoxin oxidoreductase and active molybdenum- or vanadium-containing isoenzymes are not expressed when the cells are grown in the presence of these other metals
Pyrococcus furiosus
Purification (Commentary) (protein specific)
Commentary
Organism
enzyme from vanadium- and molybdenum-grown cells
Pyrococcus furiosus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
formaldehyde + H2O + oxidized benzyl viologen
-
722490
Pyrococcus furiosus
formate + H+ + reduced benzyl viologen
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
80
-
assay at
Pyrococcus furiosus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.4
-
assay at
Pyrococcus furiosus
Other publictions for EC 1.2.7.B2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740555
Smalley
Functional and genomic diversi ...
Methyloversatilis discipulorum, Methyloversatilis discipulorum RZ18-153, Methyloversatilis discipulorum FAM1, Methyloversatilis discipulorum RZ94
Int. J. Syst. Evol. Microbiol.
65
2227-2233
2015
-
-
-
-
-
-
-
-
-
-
-
4
-
11
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722823
Liao
Why is the molybdenum-substitu ...
Pyrococcus furiosus
J. Biol. Inorg. Chem.
18
175-181
2013
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722893
Liao
Tungsten-dependent formaldehyd ...
Pyrococcus furiosus
J. Inorg. Biochem.
105
927-936
2011
-
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
745210
Sevcenco
Molybdenum incorporation in t ...
Pyrococcus furiosus
J. Bacteriol.
192
4143-4152
2010
-
-
-
-
-
-
-
-
-
1
1
-
-
1
-
-
-
-
-
-
-
-
1
1
3
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
723098
Sevcenco
The tungsten metallome of Pyro ...
Pyrococcus furiosus
Metallomics
1
395-402
2009
-
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
674933
Bol
Redox chemistry of tungsten an ...
Pyrococcus furiosus
J. Biol. Inorg. Chem.
11
999-1006
2006
1
-
-
-
-
-
-
6
-
3
-
-
-
6
-
-
-
-
-
-
-
-
3
-
1
1
-
-
2
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
6
-
3
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
2
2
-
-
-
-
-
-
-
2
2
674251
Bevers
WOR5, a novel tungsten-contain ...
Pyrococcus furiosus
J. Bacteriol.
187
7056-7061
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
644691
Roy
Aldehyde oxidoreductases from ...
Pyrococcus furiosus
Methods Enzymol.
331
132-144
2001
-
-
-
-
-
-
-
7
-
-
-
-
-
1
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
745202
Adams
Key role for sulfur in peptid ...
Pyrococcus furiosus
J. Bacteriol.
183
716-724
2001
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
722824
Dhawan
Spectroscopic studies of the t ...
Thermococcus litoralis, Thermococcus litoralis DSM 5473
J. Biol. Inorg. Chem.
5
313-327
2000
-
-
-
-
-
-
-
-
-
2
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644689
Roy
Purification and molecular cha ...
Pyrococcus furiosus
J. Bacteriol.
181
1171-1180
1999
1
-
-
-
-
-
3
8
-
3
2
-
-
6
1
-
1
-
-
-
1
-
9
1
2
-
-
1
7
1
1
-
1
-
-
-
1
-
-
1
-
-
-
-
3
-
8
-
3
2
-
-
1
-
1
-
-
1
-
9
1
-
-
1
7
1
1
-
-
-
-
-
-
7
7
644690
Hu
Formaldehyde ferredoxin oxidor ...
Pyrococcus furiosus
J. Mol. Biol.
286
899-914
1999
-
-
-
1
-
-
-
-
-
2
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722490
Mukund
Molybdenum and vanadium do not ...
Pyrococcus furiosus
J. Bacteriol.
178
163-167
1996
-
-
-
-
-
-
-
-
-
1
-
-
-
4
-
-
1
-
-
-
-
-
1
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
644684
Kletzin
Molecular characterization of ...
Thermococcus litoralis
J. Bacteriol.
177
4817-4819
1995
-
-
-
-
-
-
-
-
-
1
1
-
-
9
-
-
1
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
644682
Mukund
Characterization of a novel tu ...
Thermococcus litoralis, Thermococcus litoralis DSM 5473
J. Biol. Chem.
268
13592-13600
1993
-
-
-
-
-
-
3
-
-
2
2
-
-
10
1
-
1
-
-
-
1
-
11
1
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
2
2
-
-
1
-
1
-
-
1
-
11
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-