BRENDA - Enzyme Database
show all sequences of 1.2.7.B2

Purification and molecular characterization of the tungsten-containing formaldehyde ferredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus: the third of a putative five-member tungstoenzyme family

Roy, R.; Mukund, S.; Schut, G.J.; Dunn, D.M.; Weiss, R.; Adams, M.W.; J. Bacteriol. 181, 1171-1180 (1999)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Sulfide
activated up to fivefold by treatment with sulfide under reducing conditions
Pyrococcus furiosus
Inhibitors
Inhibitors
Commentary
Organism
Structure
glycerol
60% v/v, 6 h, 60% loss of activity
Pyrococcus furiosus
Glyoxal
above 1 mM
Pyrococcus furiosus
additional information
the enzyme is not inhibited when it is incubated for up to 24 h with iodoacetate, arsenite, or cyanide (each at a concentration of 5 mM) prior to being assayed under standard conditions in the absence of these reagents
Pyrococcus furiosus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
oxidized ferredoxin
80°C, pH 8.4
Pyrococcus furiosus
0.8
-
glutaric dialdehyde
80°C, pH 8.4
Pyrococcus furiosus
8
-
Succinic semialdehyde
80°C, pH 8.4
Pyrococcus furiosus
15
-
phenylpropionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
25
-
formaldehyde
80°C, pH 8.4
Pyrococcus furiosus
25
-
Indole-3-acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
60
-
acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
62
-
propionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Calcium
contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit
Pyrococcus furiosus
Iron
contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit
Pyrococcus furiosus
Tungsten
tungsten-containing enzyme. The enzyme contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit
Pyrococcus furiosus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68700
-
4 * 68700, calculated from sequence
Pyrococcus furiosus
275000
-
gel filtration
Pyrococcus furiosus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pyrococcus furiosus
O93738
-
-
Oxidation Stability
Oxidation Stability
Organism
enzyme as purified under standard conditions is oxygen sensitive, with the t50% being about 12 h when the enzyme is exposed to air. No activity is lost under anaerobic conditions
Pyrococcus furiosus
Purification (Commentary)
Commentary
Organism
-
Pyrococcus furiosus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
58
-
pH 8.4, 90°C
Pyrococcus furiosus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
acetate + reduced benzyl viologen + H+
-
-
-
?
formaldehyde + H2O + 2 oxidized ferredoxin
formaldehyde is unlikely to be the physiologiocal substrate (Km-value: 25 mM)
644689
Pyrococcus furiosus
formate + 2 reduced ferredoxin + 2 H+
-
-
-
?
formaldehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
formate + reduced benzyl viologen + H+
-
-
-
?
glutaric dialdehyde + H2O + oxidized benzyl viologen
best substrate, C5- or C6-type aldehydes might be physiologically relevant substrates
644689
Pyrococcus furiosus
? + reduced benzyl viologen + H+
-
-
-
?
indole-3-acetaldehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
indole-3-acetate + reduced benzyl viologen + H+
-
-
-
?
additional information
low activity with butyraldehyde, crotonaldehyde, no activity with: isovaleraldehyde, benzaldehyde, salicaldehyde, 2-furfuraldehyde. It does not oxidize lactate, 2-hydroxybutyrate, 2-hydroxyvalerate, 2-hydroxycaproate, or 1-ethyl-2-hydroxycaproate and it does not reduce pyruvate, 2-oxobutyrate, 2-oxovalerate, 1-ethyl-3-oxovalerate, 1-ethyl-4-oxovalerate, or 2-oxocaproate
644689
Pyrococcus furiosus
?
-
-
-
-
phenylpropionaldehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
phenylpropionate + reduced benzyl viologen + H+
-
-
-
?
propionaldehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
propionate + reduced benzyl viologen + H+
-
-
-
?
succinic semialdehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
succinate + reduced benzyl viologen + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 68700, calculated from sequence
Pyrococcus furiosus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
80
-
8 h, 50% loss of activity
Pyrococcus furiosus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2300
-
Indole-3-acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
5700
-
Succinic semialdehyde
80°C, pH 8.4
Pyrococcus furiosus
11000
-
propionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
25000
-
phenylpropionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
42000
-
glutaric dialdehyde
80°C, pH 8.4
Pyrococcus furiosus
44000
-
acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
71000
-
formaldehyde
80°C, pH 8.4
Pyrococcus furiosus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
-
the activity of the pure enzyme with benzyl viologen as the electron acceptor increases linearly with pH over the pH range 5.5 (1.5 U/mg) to 10.0 (55 U/mg) at 80°C
Pyrococcus furiosus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
10
the activity of the pure enzyme with benzyl viologen as the electron acceptor increases linearly with pH over the pH range 5.5 (1.5 U/mg) to 10.0 (55 U/mg) at 80°C
Pyrococcus furiosus
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
the enzyme contains a pterin cofactor
Pyrococcus furiosus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Sulfide
activated up to fivefold by treatment with sulfide under reducing conditions
Pyrococcus furiosus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
the enzyme contains a pterin cofactor
Pyrococcus furiosus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
glycerol
60% v/v, 6 h, 60% loss of activity
Pyrococcus furiosus
Glyoxal
above 1 mM
Pyrococcus furiosus
additional information
the enzyme is not inhibited when it is incubated for up to 24 h with iodoacetate, arsenite, or cyanide (each at a concentration of 5 mM) prior to being assayed under standard conditions in the absence of these reagents
Pyrococcus furiosus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
oxidized ferredoxin
80°C, pH 8.4
Pyrococcus furiosus
0.8
-
glutaric dialdehyde
80°C, pH 8.4
Pyrococcus furiosus
8
-
Succinic semialdehyde
80°C, pH 8.4
Pyrococcus furiosus
15
-
phenylpropionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
25
-
formaldehyde
80°C, pH 8.4
Pyrococcus furiosus
25
-
Indole-3-acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
60
-
acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
62
-
propionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Calcium
contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit
Pyrococcus furiosus
Iron
contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit
Pyrococcus furiosus
Tungsten
tungsten-containing enzyme. The enzyme contains approximately 1 W atom, 4 Fe atoms, and 1 Ca atom per subunit
Pyrococcus furiosus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
68700
-
4 * 68700, calculated from sequence
Pyrococcus furiosus
275000
-
gel filtration
Pyrococcus furiosus
Oxidation Stability (protein specific)
Oxidation Stability
Organism
enzyme as purified under standard conditions is oxygen sensitive, with the t50% being about 12 h when the enzyme is exposed to air. No activity is lost under anaerobic conditions
Pyrococcus furiosus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pyrococcus furiosus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
58
-
pH 8.4, 90°C
Pyrococcus furiosus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetaldehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
acetate + reduced benzyl viologen + H+
-
-
-
?
formaldehyde + H2O + 2 oxidized ferredoxin
formaldehyde is unlikely to be the physiologiocal substrate (Km-value: 25 mM)
644689
Pyrococcus furiosus
formate + 2 reduced ferredoxin + 2 H+
-
-
-
?
formaldehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
formate + reduced benzyl viologen + H+
-
-
-
?
glutaric dialdehyde + H2O + oxidized benzyl viologen
best substrate, C5- or C6-type aldehydes might be physiologically relevant substrates
644689
Pyrococcus furiosus
? + reduced benzyl viologen + H+
-
-
-
?
indole-3-acetaldehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
indole-3-acetate + reduced benzyl viologen + H+
-
-
-
?
additional information
low activity with butyraldehyde, crotonaldehyde, no activity with: isovaleraldehyde, benzaldehyde, salicaldehyde, 2-furfuraldehyde. It does not oxidize lactate, 2-hydroxybutyrate, 2-hydroxyvalerate, 2-hydroxycaproate, or 1-ethyl-2-hydroxycaproate and it does not reduce pyruvate, 2-oxobutyrate, 2-oxovalerate, 1-ethyl-3-oxovalerate, 1-ethyl-4-oxovalerate, or 2-oxocaproate
644689
Pyrococcus furiosus
?
-
-
-
-
phenylpropionaldehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
phenylpropionate + reduced benzyl viologen + H+
-
-
-
?
propionaldehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
propionate + reduced benzyl viologen + H+
-
-
-
?
succinic semialdehyde + H2O + oxidized benzyl viologen
-
644689
Pyrococcus furiosus
succinate + reduced benzyl viologen + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 68700, calculated from sequence
Pyrococcus furiosus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
80
-
8 h, 50% loss of activity
Pyrococcus furiosus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2300
-
Indole-3-acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
5700
-
Succinic semialdehyde
80°C, pH 8.4
Pyrococcus furiosus
11000
-
propionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
25000
-
phenylpropionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
42000
-
glutaric dialdehyde
80°C, pH 8.4
Pyrococcus furiosus
44000
-
acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
71000
-
formaldehyde
80°C, pH 8.4
Pyrococcus furiosus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
-
the activity of the pure enzyme with benzyl viologen as the electron acceptor increases linearly with pH over the pH range 5.5 (1.5 U/mg) to 10.0 (55 U/mg) at 80°C
Pyrococcus furiosus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
10
the activity of the pure enzyme with benzyl viologen as the electron acceptor increases linearly with pH over the pH range 5.5 (1.5 U/mg) to 10.0 (55 U/mg) at 80°C
Pyrococcus furiosus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
90
-
Indole-3-acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
170
-
propionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
710
-
Succinic semialdehyde
80°C, pH 8.4
Pyrococcus furiosus
730
-
acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1700
-
phenylpropionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
3000
-
formaldehyde
80°C, pH 8.4
Pyrococcus furiosus
60000
-
glutaric dialdehyde
80°C, pH 8.4
Pyrococcus furiosus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
90
-
Indole-3-acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
170
-
propionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
710
-
Succinic semialdehyde
80°C, pH 8.4
Pyrococcus furiosus
730
-
acetaldehyde
80°C, pH 8.4
Pyrococcus furiosus
1700
-
phenylpropionaldehyde
80°C, pH 8.4
Pyrococcus furiosus
3000
-
formaldehyde
80°C, pH 8.4
Pyrococcus furiosus
60000
-
glutaric dialdehyde
80°C, pH 8.4
Pyrococcus furiosus
Other publictions for EC 1.2.7.B2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740555
Smalley
Functional and genomic diversi ...
Methyloversatilis discipulorum, Methyloversatilis discipulorum FAM1, Methyloversatilis discipulorum RZ18-153, Methyloversatilis discipulorum RZ94
Int. J. Syst. Evol. Microbiol.
65
2227-2233
2015
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10
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4
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722823
Liao
Why is the molybdenum-substitu ...
Pyrococcus furiosus
J. Biol. Inorg. Chem.
18
175-181
2013
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1
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1
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722893
Liao
Tungsten-dependent formaldehyd ...
Pyrococcus furiosus
J. Inorg. Biochem.
105
927-936
2011
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745210
Sevcenco
Molybdenum incorporation in t ...
Pyrococcus furiosus
J. Bacteriol.
192
4143-4152
2010
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1
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1
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1
1
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723098
Sevcenco
The tungsten metallome of Pyro ...
Pyrococcus furiosus
Metallomics
1
395-402
2009
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1
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3
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1
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1
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1
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674933
Bol
Redox chemistry of tungsten an ...
Pyrococcus furiosus
J. Biol. Inorg. Chem.
11
999-1006
2006
1
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6
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2
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5
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3
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1
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2
1
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1
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6
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3
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3
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1
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2
2
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2
2
674251
Bevers
WOR5, a novel tungsten-contain ...
Pyrococcus furiosus
J. Bacteriol.
187
7056-7061
2005
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1
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3
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3
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1
1
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644691
Roy
Aldehyde oxidoreductases from ...
Pyrococcus furiosus
Methods Enzymol.
331
132-144
2001
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7
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1
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9
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7
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9
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745202
Adams
Key role for sulfur in peptid ...
Pyrococcus furiosus
J. Bacteriol.
183
716-724
2001
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1
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1
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