KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
CoA | pH 8.0, 65°C, anaerobic | Methanothermobacter thermautotrophicus | |
1.3 | - |
2-ketoisovalerate | pH 8.0, 65°C, anaerobic | Methanothermobacter thermautotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
x * 55000 + x * 37000 + x * 15000, SDS-PAGE | Methanothermobacter thermautotrophicus |
37000 | - |
x * 55000 + x * 37000 + x * 15000, SDS-PAGE | Methanothermobacter thermautotrophicus |
55000 | - |
x * 55000 + x * 37000 + x * 15000, SDS-PAGE | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pyrococcus furiosus | - |
? | - |
? | |
additional information | Thermococcus litoralis | - |
? | - |
? | |
additional information | Methanothermobacter thermautotrophicus | enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium | ? | - |
? | |
additional information | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
strain DSM 2133 | - |
Pyrococcus furiosus | - |
- |
- |
Thermococcus litoralis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
under anaerobic conditions | Methanothermobacter thermautotrophicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 | Methanothermobacter thermautotrophicus | |
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 | Pyrococcus furiosus | |
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 | Thermococcus litoralis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.8 | - |
- |
Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-ketobutyrate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | propanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
2-ketobutyrate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | propanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
2-ketobutyrate + CoA + oxidized ferredoxin | benzyl viologen as electron acceptor | Methanothermobacter thermautotrophicus | propanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
2-ketobutyrate + CoA + oxidized ferredoxin | benzyl viologen as electron acceptor | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | propanoyl-CoA + CO2 + reduced ferredoxin | - |
? | |
2-ketoisohexanoate + CoA + benzyl viologen | - |
Methanothermobacter thermautotrophicus | isopentanoyl-CoA + CO2 + reduced benzyl viologen | - |
? | |
2-ketoisovalerate + CoA + benzyl viologen | - |
Methanothermobacter thermautotrophicus | S-(2-methylpropanoyl)-CoA + CO2 + reduced benzyl viologen | - |
? | |
2-ketoisovalerate + CoA + benzyl viologen | - |
Methanothermobacter thermautotrophicus Marburg / DSM 2133 | S-(2-methylpropanoyl)-CoA + CO2 + reduced benzyl viologen | - |
? | |
2-ketovalerate + CoA + benzyl viologen | - |
Methanothermobacter thermautotrophicus | butanoyl-CoA + CO2 + reduced benzyl viologen | - |
? | |
additional information | - |
Pyrococcus furiosus | ? | - |
? | |
additional information | - |
Thermococcus litoralis | ? | - |
? | |
additional information | no substrate are 2-ketoglutarate and pyruvate | Methanothermobacter thermautotrophicus | ? | - |
? | |
additional information | enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium | Methanothermobacter thermautotrophicus | ? | - |
? | |
additional information | no substrate are 2-ketoglutarate and pyruvate | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | ? | - |
? | |
additional information | enzyme is involved in the biosynthesis of amino acids from fatty acids take up from the grown medium | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | ? | - |
? | |
phenylpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
phenylpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
phenylpyruvate + CoA + oxidized ferredoxin | benzyl viologen as electron acceptor | Methanothermobacter thermautotrophicus | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
? | |
phenylpyruvate + CoA + oxidized ferredoxin | benzyl viologen as electron acceptor | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000 + x * 37000 + x * 15000, SDS-PAGE | Methanothermobacter thermautotrophicus |
? | comparison of N-terminal amino acid sequences | Methanothermobacter thermautotrophicus |
? | comparison of N-terminal amino acid sequences | Pyrococcus furiosus |
? | comparison of N-terminal amino acid sequences | Thermococcus litoralis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
at pH 8.0 | Methanothermobacter thermautotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.7 | - |
at 65°C | Methanothermobacter thermautotrophicus |