Activating Compound | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | requires for maximal activity of enzyme | Pyrococcus furiosus | |
thiamine diphosphate | requires for maximal activity of enzyme | Thermococcus litoralis |
Cloned (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Pyrococcus furiosus | |
additional information | - |
additional information | - |
Thermococcus litoralis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | each of the tetramer contains three [4Fe-4S] cluster | Pyrococcus furiosus | |
Fe | each of the tetramer contains three [4Fe-4S] cluster | Thermococcus litoralis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
13000 | - |
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE | Thermococcus litoralis |
23000 | - |
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE | Thermococcus litoralis |
34000 | - |
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE | Thermococcus litoralis |
47000 | - |
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE | Thermococcus litoralis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pyrococcus furiosus | enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids | ? | - |
? | |
additional information | Thermococcus litoralis | enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Thermococcus litoralis | - |
- |
- |
Oxidation Stability | Organism |
---|---|
enzyme is irreversibly inactivated by oxygen | Pyrococcus furiosus |
Purification (Comment) | Organism |
---|---|
under anaerobic conditions | Pyrococcus furiosus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | pathway | Pyrococcus furiosus | |
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | pathway | Thermococcus litoralis | |
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 | Pyrococcus furiosus | |
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ | this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 | Thermococcus litoralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-ketobutyrate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | propanoyl-CoA + CO2 + reduced ferredoxin | - |
r | |
2-ketobutyrate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | propanoyl-CoA + CO2 + reduced ferredoxin | - |
r | |
2-ketoisocaproate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | S-(3-methylbutanoyl)-CoA + CO2 + reduced ferredoxin | - |
r | |
2-ketoisocaproate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | S-(3-methylbutanoyl)-CoA + CO2 + reduced ferredoxin | - |
r | |
2-ketoisovalerate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin | - |
r | |
2-ketoisovalerate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin | - |
? | |
4-methylthio-2-oxobutanoate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | 3-methylthio-2-oxopropanoyl-CoA + CO2 + reduced ferredoxin | - |
r | |
4-methylthio-2-oxobutanoate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | 3-methylthio-2-oxopropanoyl-CoA + CO2 + reduced ferredoxin | - |
r | |
hydroxyphenylpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
hydroxyphenylpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
indolpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | indolacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
indolpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | indolacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
additional information | enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids | Pyrococcus furiosus | ? | - |
? | |
additional information | enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids | Thermococcus litoralis | ? | - |
? | |
additional information | enzyme catalyses also the nonoxidative decarboxylation of pyruvate to acetaldehyde | Pyrococcus furiosus | ? | - |
? | |
additional information | enzyme catalyses also the nonoxidative decarboxylation of pyruvate to acetaldehyde | Thermococcus litoralis | ? | - |
? | |
additional information | no substrate is 2-ketoglutarate | Pyrococcus furiosus | ? | - |
? | |
additional information | no substrate is 2-ketoglutarate | Thermococcus litoralis | ? | - |
? | |
phenylglyoxylate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | benzoyl-CoA + CO2 + reduced ferredoxin | - |
r | |
phenylglyoxylate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | benzoyl-CoA + CO2 + reduced ferredoxin | - |
r | |
phenylpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
phenylpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
pyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
pyruvate + CoA + oxidized ferredoxin | - |
Thermococcus litoralis | acetyl-CoA + CO2 + reduced ferredoxin | - |
r |
Subunits | Comment | Organism |
---|---|---|
octamer | alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE | Thermococcus litoralis |