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Literature summary for 1.2.7.7 extracted from

  • Schut, G.J.; Menon, A.L.; Adams, M.W.W.
    2-Keto acid oxidoreductases from Pyrococcus furiosus and Thermococcus litoralis (2001), Methods Enzymol., 331, 144-158.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
thiamine diphosphate requires for maximal activity of enzyme Pyrococcus furiosus
thiamine diphosphate requires for maximal activity of enzyme Thermococcus litoralis

Cloned(Commentary)

Cloned (Comment) Organism
-
Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Pyrococcus furiosus
additional information
-
additional information
-
Thermococcus litoralis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe each of the tetramer contains three [4Fe-4S] cluster Pyrococcus furiosus
Fe each of the tetramer contains three [4Fe-4S] cluster Thermococcus litoralis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13000
-
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Thermococcus litoralis
23000
-
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Thermococcus litoralis
34000
-
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Thermococcus litoralis
47000
-
alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Thermococcus litoralis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pyrococcus furiosus enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids ?
-
?
additional information Thermococcus litoralis enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids ?
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-
Thermococcus litoralis
-
-
-

Oxidation Stability

Oxidation Stability Organism
enzyme is irreversibly inactivated by oxygen Pyrococcus furiosus

Purification (Commentary)

Purification (Comment) Organism
under anaerobic conditions Pyrococcus furiosus

Reaction

Reaction Comment Organism Reaction ID
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ pathway Pyrococcus furiosus
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ pathway Thermococcus litoralis
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 Pyrococcus furiosus
3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + 2 H+ this enzyme is one of four 2-oxoacid oxidoreductases: indolepyruvate oxidoreductase, EC 1.2.7.8, pyruvate oxidoreductase, EC 1.2.7.1., 2-oxogluterate oxidoreductase, EC 1.2.7.3 Thermococcus litoralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-ketobutyrate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus propanoyl-CoA + CO2 + reduced ferredoxin
-
r
2-ketobutyrate + CoA + oxidized ferredoxin
-
Thermococcus litoralis propanoyl-CoA + CO2 + reduced ferredoxin
-
r
2-ketoisocaproate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus S-(3-methylbutanoyl)-CoA + CO2 + reduced ferredoxin
-
r
2-ketoisocaproate + CoA + oxidized ferredoxin
-
Thermococcus litoralis S-(3-methylbutanoyl)-CoA + CO2 + reduced ferredoxin
-
r
2-ketoisovalerate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin
-
r
2-ketoisovalerate + CoA + oxidized ferredoxin
-
Thermococcus litoralis S-(2-methylpropanoyl)-CoA + CO2 + reduced ferredoxin
-
?
4-methylthio-2-oxobutanoate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus 3-methylthio-2-oxopropanoyl-CoA + CO2 + reduced ferredoxin
-
r
4-methylthio-2-oxobutanoate + CoA + oxidized ferredoxin
-
Thermococcus litoralis 3-methylthio-2-oxopropanoyl-CoA + CO2 + reduced ferredoxin
-
r
hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin
-
r
hydroxyphenylpyruvate + CoA + oxidized ferredoxin
-
Thermococcus litoralis 4-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin
-
r
indolpyruvate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus indolacetyl-CoA + CO2 + reduced ferredoxin
-
r
indolpyruvate + CoA + oxidized ferredoxin
-
Thermococcus litoralis indolacetyl-CoA + CO2 + reduced ferredoxin
-
r
additional information enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids Pyrococcus furiosus ?
-
?
additional information enzyme catalyses the CoA-dependent oxidation of branched-chain 2-ketoacids Thermococcus litoralis ?
-
?
additional information enzyme catalyses also the nonoxidative decarboxylation of pyruvate to acetaldehyde Pyrococcus furiosus ?
-
?
additional information enzyme catalyses also the nonoxidative decarboxylation of pyruvate to acetaldehyde Thermococcus litoralis ?
-
?
additional information no substrate is 2-ketoglutarate Pyrococcus furiosus ?
-
?
additional information no substrate is 2-ketoglutarate Thermococcus litoralis ?
-
?
phenylglyoxylate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus benzoyl-CoA + CO2 + reduced ferredoxin
-
r
phenylglyoxylate + CoA + oxidized ferredoxin
-
Thermococcus litoralis benzoyl-CoA + CO2 + reduced ferredoxin
-
r
phenylpyruvate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus phenylacetyl-CoA + CO2 + reduced ferredoxin
-
r
phenylpyruvate + CoA + oxidized ferredoxin
-
Thermococcus litoralis phenylacetyl-CoA + CO2 + reduced ferredoxin
-
r
pyruvate + CoA + oxidized ferredoxin
-
Pyrococcus furiosus acetyl-CoA + CO2 + reduced ferredoxin
-
r
pyruvate + CoA + oxidized ferredoxin
-
Thermococcus litoralis acetyl-CoA + CO2 + reduced ferredoxin
-
r

Subunits

Subunits Comment Organism
octamer alpha,beta,gamma,delta, 2 * 47000 + 2 * 34000 + 2 * 23000 + 2 * 13000, SDS PAGE Thermococcus litoralis