Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ti(III)citrate | 1mM for 1 min at 65°C, 45fold increase in initial activity | Carboxydothermus hydrogenoformans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO | 45% CO in headspace, 50% inhibition | Carboxydothermus hydrogenoformans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Carboxydothermus hydrogenoformans | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 61.5 mol nonheme iron per mol of complex | Carboxydothermus hydrogenoformans | |
Nickel | 3 mol per mol of complex | Carboxydothermus hydrogenoformans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
2 * 62000, enzyme, + electron transfer protein CooF and membrane bound [Ni-Fe] hydrogenase, six differentsubunits | Carboxydothermus hydrogenoformans |
62000 | - |
2 * 62000, SDS-PAGE, enzyme | Carboxydothermus hydrogenoformans |
120000 | - |
gel filtration, enzyme | Carboxydothermus hydrogenoformans |
450000 | - |
gel filtration, complex | Carboxydothermus hydrogenoformans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carboxydothermus hydrogenoformans | - |
enzyme CooS in complex with an electron transfer protein CooF and a membrane bound [Ni-Fe] hydrogenase | - |
Purification (Comment) | Organism |
---|---|
- |
Carboxydothermus hydrogenoformans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5924 | - |
pH 7.0, 70°C | Carboxydothermus hydrogenoformans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO + H2O + methyl viologen | optimal concentration of CO 5% in headspace | Carboxydothermus hydrogenoformans | CO2 + reduced methyl viologen | - |
? | |
H2 + reduced methyl viologen | - |
Carboxydothermus hydrogenoformans | methyl viologen | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 62000, SDS-PAGE, enzyme | Carboxydothermus hydrogenoformans |
octamer | 2 * 62000, enzyme, + electron transfer protein CooF and membrane bound [Ni-Fe] hydrogenase, six differentsubunits | Carboxydothermus hydrogenoformans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
both for enzyme and complex | Carboxydothermus hydrogenoformans |