Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, enzyme Ape2126/2128 | Aeropyrum pernix |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | 1020% inhibition is observed with 50 mM KCl, while with higher concentrations (maximum, 0.6 M), 4665% activation is reached, and further increased concentrations of KCl are inhibitory, enzyme Ape2126/2128 | Aeropyrum pernix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.24 | - |
pyruvate | pH 8.5, 80°C, enzyme Ape2126/2128 | Aeropyrum pernix |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34936 | - |
1 * 71639 + 1 * 34936, calculated from sequence, enzyme Ape2126/2128 | Aeropyrum pernix |
35000 | - |
1 * 72000 + 1 * 35000, SDS-PAGE, enzyme Ape2126/2128 | Aeropyrum pernix |
71639 | - |
1 * 71639 + 1 * 34936, calculated from sequence, enzyme Ape2126/2128 | Aeropyrum pernix |
72000 | - |
1 * 72000 + 1 * 35000, SDS-PAGE, enzyme Ape2126/2128 | Aeropyrum pernix |
110000 | - |
gel filtration, enzyme Ape2126/2128 | Aeropyrum pernix |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | Q9YA13 and Q9YA11 | Q9YA13: alpha-subunit, Q9YA11: beta-subunit | - |
Purification (Comment) | Organism |
---|---|
enzyme Ape2126/2128 | Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-4-methyl-thio-butyrate + CoA + 2 methyl viologen | enzyme Ape2126/2128, 37% of the activity compared to pyruvate | Aeropyrum pernix | ? | - |
? | |
2-oxoadipate + CoA + 2 methyl viologen | enzyme Ape2126/2128, 37% of the activity compared to pyruvate | Aeropyrum pernix | ? | - |
? | |
2-oxobutyrate + CoA + 2 oxidized methyl viologen | enzyme Ape2126/2128, 98% of the activity compared to pyruvate | Aeropyrum pernix | propanoyl-CoA + CO2 + 2 reduced methyl viologen | - |
? | |
glyoxylate + CoA + 2 oxidized methyl viologen | enzyme Ape2126/2128, 89% of the activity compared to pyruvate | Aeropyrum pernix | ? | - |
? | |
hydroxypyruvate + CoA + 2 methyl viologen | enzyme Ape2126/2128, 38% of the activity compared to pyruvate | Aeropyrum pernix | ? | - |
? | |
additional information | activity with 2-oxoglutarate is 6% compared to the activity with pyruvate. No activity with 3-methyl-2-oxovalerate, 4-methyl-2-oxovalerate, 2-oxoisocaproic acid or 2-oxooctanoic acid, enzyme Ape2126/2128, | Aeropyrum pernix | ? | - |
? | |
pyruvate + CoA + 2 oxidized methyl viologen | enzyme Ape2126/2128 | Aeropyrum pernix | acetyl-CoA + CO2 + 2 reduced methyl viologen + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 71639 + 1 * 34936, calculated from sequence, enzyme Ape2126/2128 | Aeropyrum pernix |
heterodimer | 1 * 72000 + 1 * 35000, SDS-PAGE, enzyme Ape2126/2128 | Aeropyrum pernix |
Synonyms | Comment | Organism |
---|---|---|
Ape2126/2128 | ordered locus name | Aeropyrum pernix |
OFOR | - |
Aeropyrum pernix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
assay at | Aeropyrum pernix |
105 | - |
enzyme Ape2126/2128 | Aeropyrum pernix |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | 110 | 80°C: 45% of maximal activity, 110°C: 95% of maximal activity, enzyme Ape2126/2128 | Aeropyrum pernix |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
20 min, stable, enzyme Ape2126/2128 | Aeropyrum pernix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
enzyme Ape2126/2128 | Aeropyrum pernix |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | pH 6.0: about 45% of maximal activity, pH 10.0: about 60% of maximal activity, enzyme Ape2126/2128 | Aeropyrum pernix |