Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Mycolicibacterium smegmatis |
Crystallization (Comment) | Organism |
---|---|
enzyme in complex with phosphono ethyl succinyl phosphonate and/or succinyl phosphonate, hanging drop vapor diffusion method, using 2% (w/v) 2-methyl-2-propanol and 47% (w/v) 2-methyl-2,4-pentanediol | Mycolicibacterium smegmatis |
in complex with inhibitors succinyl phosphonate, phosphono ethyl succinyl phosphonate. Crystallographic snapshots of the Michaelis-like complex, followed by the covalent adduction of the inhibitor to thiamin diphosphate, mimicking the pre-decarboxylation complex | Mycolicibacterium smegmatis |
Protein Variants | Comment | Organism |
---|---|---|
E952Q | in this mutant the activation of thiamine diphosphate is impaired | Mycolicibacterium smegmatis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phosphono ethyl succinyl phosphonate | competitive inhibitor | Mycolicibacterium smegmatis | |
succinyl phosphonate | competitive inhibitor | Mycolicibacterium smegmatis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 1 mM used in assay conditions | Mycolicibacterium smegmatis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | A0R2B1 | - |
- |
Mycolicibacterium smegmatis | A0R2B1 | cf. EC 4.1.1.71, EC 2.2.1.5 and EC 2.3.1.61 | - |
Mycolicibacterium smegmatis ATCC 700084 | A0R2B1 | cf. EC 4.1.1.71, EC 2.2.1.5 and EC 2.3.1.61 | - |
Mycolicibacterium smegmatis mc(2)155 | A0R2B1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + 2,6-dichlorophenolindophenol | - |
Mycolicibacterium smegmatis | ? | - |
? | |
2-oxoglutarate + 2,6-dichlorophenolindophenol | - |
Mycolicibacterium smegmatis ATCC 700084 | ? | - |
? | |
2-oxoglutarate + 2,6-dichlorophenolindophenol | - |
Mycolicibacterium smegmatis mc(2)155 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Kgd | thiamine diphosphate-dependent E1o component of the 2-oxoglutarate dehydrogenase complex | Mycolicibacterium smegmatis |
SucA | - |
Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Mycolicibacterium smegmatis | |
thiamine diphosphate | dependent on | Mycolicibacterium smegmatis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
succinyl phosphonate | at pH 7.0 and 26°C | Mycolicibacterium smegmatis | |
0.043 | - |
succinyl phosphonate | pH 7. 26°C | Mycolicibacterium smegmatis | |
0.88 | - |
phosphono ethyl succinyl phosphonate | at pH 7.0 and 26°C | Mycolicibacterium smegmatis | |
0.88 | - |
phosphono ethyl succinyl phosphonate | pH 7, 26°C | Mycolicibacterium smegmatis |